Invitrogen™

FM™ 1-43 Dye (N-(3-Triethylammoniumpropyl)-4-(4-(Dibutylamino) Styryl) Pyridinium Dibromide)

Catalog number:  T3163

Related applications:

Cell Tracing & Tracking

| Cell Viability, Proliferation & Function

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Description

FM 1-43 membrane probe is an excellent reagent both for identifying actively firing neurons and for investigating the mechanisms of activity-dependent vesicle cycling. This water-soluble dye, which is nontoxic to cells and virtually nonfluorescent in aqueous medium, is believed to insert into the outer leaflet of the cell membrane where it becomes intensely fluorescent. In a neuron that is actively releasing neurotransmitters, the dye becomes internalized within the recycled synaptic vesicles and the nerve terminals become brightly stained. The nonspecific staining of cell-surface membranes can simply be washed off prior to viewing. FM 1-43 membrane probe is also available as 1 mg in a single vial (T-3163). FM 1-43FX membrane probe (F-35355), an analog of FM 1-43 membrane probe that can be fixed in place using aldehyde-based fixatives, is also available.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Shipping Condition: Room Temperature
Sub Cellular Localization: Plasma Membrane
Detection Method: Fluorescent
For Use With (Equipment): Fluorescence Microscope
Quantity: 1mg
Color: Orange

Contents & storage

Store at room temperature and protect from light.