TC-FlAsH™ II In-Cell Tetracysteine Tag Detection Kit contains an expression tag-based fluorescence labeling reagent for live-cell labeling. Mammalian cell lines expressing a protein fused to a tetracysteine tag (CCPGCC) can be labeled with the green-fluorescent FlAsH-EDT2 reagent contained in the kit. The tagged protein is fluorescent only when the labeling reagent is added. BAL wash buffer replaces the previously supplied Disperse Blue 3 and EDT-based wash buffer as an olfactorily more agreeable reagent that yields superior signal to noise.
The kit contains FlAsH-EDT2 labeling reagent (store at -20°C, protected from light) and BAL wash buffer (stored at 4°C). When stored as directed, the kit is stable for 6 months.