TC-ReAsH™ II In-Cell Tetracysteine Tag Detection Kit (Red Fluorescence), for live-cell imaging
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Invitrogen™

TC-ReAsH™ II In-Cell Tetracysteine Tag Detection Kit (Red Fluorescence), for live-cell imaging

TC-ReAsH™ II In-Cell Tetracysteine Tag Detection Kit contains an expression tag-based fluorescence labeling reagent for live-cell labeling. Mammalian cell linesRead more
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Catalog NumberQuantity
T345621 Kit
Catalog number T34562
Price (USD)
1,440.00
1 kit
Add to cart
Quantity:
1 Kit
Price (USD)
1,440.00
1 kit
Add to cart
TC-ReAsH™ II In-Cell Tetracysteine Tag Detection Kit contains an expression tag-based fluorescence labeling reagent for live-cell labeling. Mammalian cell lines expressing a protein fused to a tetracysteine tag (CCPGCC) can be labeled with the red-fluorescent ReAsH-EDT2 reagent contained in the kit. The tagged protein is fluorescent only when the labeling reagent is added. BAL wash buffer replaces the previously supplied Disperse Blue 3 and EDT-based wash buffer as an olfactorily more agreeable reagent that yields superior signal to noise.


The kit contains ReAsH-EDT2 labeling reagent (store at -20°C, protected from light) and BAL wash buffer (stored at 4°C). When stored as directed, the kit is stable for 6 months.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorRed
Detection MethodFluorescence
For Use With (Equipment)Fluorescence Microscope
Label or DyeReAsH
Product LineTC-ReAsH II
Product TypeTetracysteine Tag Detection Kit
Quantity1 Kit
Shipping ConditionWet Ice
FormatKit
Unit Size1 kit
Contents & Storage
Store in freezer (-5 to -30°C).

Frequently asked questions (FAQs)

Can fluorescent protein-expressing cells be fixed?

Yes, fluorescent protein-expressing cells can be fixed using 4% paraformaldehyde in PBS for 10 min followed by one quick PBS rinse and 3 x 5 min washes with 1 mL PBS.

Are the fluorescent proteins offered by Thermo Fisher Scientific (EmGFP, YFP, CFP, BFP and Cycle 3 GFP) humanized?

Yes, all of the fluorescent proteins offered by (EmGFP, YFP, CFP, BFP and Cycle 3 GFP) have been humanized for optimal mammalian expression.