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      Invitrogen™

      TC-ReAsH™ II In-Cell Tetracysteine Tag Detection Kit (Red Fluorescence), for live-cell imaging

      Catalog number:  T34562

       Related applications:

      Cellular Imaging

      | Labeling Chemistry

      | Protein Assays and Analysis

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      Description

      TC-ReAsH™ II In-Cell Tetracysteine Tag Detection Kit contains an expression tag-based fluorescence labeling reagent for live-cell labeling. Mammalian cell lines expressing a protein fused to a tetracysteine tag (CCPGCC) can be labeled with the red-fluorescent ReAsH-EDT2 reagent contained in the kit. The tagged protein is fluorescent only when the labeling reagent is added. BAL wash buffer replaces the previously supplied Disperse Blue 3 and EDT-based wash buffer as an olfactorily more agreeable reagent that yields superior signal to noise.


      The kit contains ReAsH-EDT2 labeling reagent (store at -20°C, protected from light) and BAL wash buffer (stored at 4°C). When stored as directed, the kit is stable for 6 months.
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Color: Red
      Detection Location: In-Cell Detection
      Detection Method: Fluorescent
      For Use With (Equipment): Fluorescence Microscope
      Label or Dye: ReAsH
      Product Line: TC-ReAsH™
      Product Size: 1 kit
      Shipping Condition: Wet Ice

      Contents & storage

      Store in freezer (-5 to -30°C).

      Documents

      Manuals & protocols