La C2 maleimida Texas Red™ reactiva al tiol puede utilizarse para crear bioconjugados de fluorescencia rojo brillante con un máximoMás información
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Número de catálogo
Cantidad
T6008
también denominado T-6008
5 mg
Número de catálogo T6008
también denominado T-6008
Precio (MXN)
-
Cantidad:
5 mg
La C2 maleimida Texas Red™ reactiva al tiol puede utilizarse para crear bioconjugados de fluorescencia rojo brillante con un máximo de excitación/emisión ∼595/615 nm.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Reactividad químicaTiol
Emisión615
Excitación595
Etiqueta o tinteTexas Red™
Tipo de productoMaleimida
Cantidad5 mg
Fracción reactivaMaleimida
Condiciones de envíoTemperatura ambiente
Tipo de etiquetaColorantes clásicos
Línea de productosTexas Red
Unit Size5 mg
Contenido y almacenamiento
Almacenar en el congelador (de – 5 a – 30 °C) y proteger de la luz.
Citations & References (16)
Citations & References
Abstract
Sampling unfolding intermediates in calmodulin by single-molecule spectroscopy.
Authors:Slaughter BD, Unruh JR, Price ES, Huynh JL, Bieber Urbauer RJ, Johnson CK
Journal:J Am Chem Soc
PubMed ID:16117552
'We used single-pair fluorescence resonance energy transfer (spFRET) measurements to characterize denatured and partially denatured states of the multidomain calcium signaling protein calmodulin (CaM) in both its apo and Ca(2+)-bound forms. The results demonstrate the existence of an unfolding intermediate. A CaM mutant (CaM-T34C-T110C) was doubly labeled with fluorescent probes ... More
An allosteric mechanism controls antigen presentation by the H-2K(b) complex.
Authors:Gakamsky DM, Boyd LF, Margulies DH, Davis DM, Strominger JL, Pecht I
Journal:Biochemistry
PubMed ID:10508421
'The mechanism of assembly/dissociation of a recombinant water-soluble class I major histocompatibility complex (MHC) H-2Kb molecule was studied by a real-time fluorescence resonance energy transfer method. Like the H-2Kd ternary complex [Gakamsky et al. (1996) Biochemistry 35, 14841-14848], the interactions among the heavy chain, beta2-microglobulin (beta2m), and antigenic peptides were ... More
High-throughput investigation of osteoblast response to polymer crystallinity: influence of nanometer-scale roughness on proliferation.
Authors:Washburn NR, Yamada KM, Simon CG, Kennedy SB, Amis EJ
Journal:Biomaterials
PubMed ID:14643595
'A high-throughput method for analyzing cellular response to crystallinity in a polymer material is presented. Variations in crystallinity lead to changes in surface roughness on nanometer length scales, and it is shown that cells are exquisitely sensitive to these changes. Gradients of polymer crystallinity were fabricated on films of poly(L-lactic ... More
Fluorescence labeling, purification, and immobilization of a double cysteine mutant calmodulin fusion protein for single-molecule experiments.
Authors:Allen MW, Urbauer RJ, Zaidi A, Williams TD, Urbauer JL, Johnson CK
Journal:Anal Biochem
PubMed ID:14751262
'We present a method of labeling and immobilizing a low-molecular-weight protein, calmodulin (CaM), by fusion to a larger protein, maltose binding protein (MBP), for single-molecule fluorescence experiments. Immobilization in an agarose gel matrix eliminates potential interactions of the protein and the fluorophore(s) with a glass surface and allows prolonged monitoring ... More
Influence of ADP on cross-bridge-dependent activation of myofibrillar thin filaments.
Authors:Zhang D, Yancey KW, Swartz DR
Journal:Biophys J
PubMed ID:10827987
'Contraction of skeletal muscle is regulated by calcium at the level of the thin filament via troponin and tropomyosin. Studies have indicated that strong cross-bridge binding is also involved in activation of the thin filament. To further test this, myofibrils were incubated with a wide range of fluorescent myosin subfragment ... More