TetraSpeck™ Microspheres, 0.5 μm, fluorescent blue/green/orange/dark red

Citas y referencias (16)

Invitrogen™

TetraSpeck™ Microspheres, 0.5 μm, fluorescent blue/green/orange/dark red

Las microesferas TetraSpec™ de 0,5 µm están completamente teñidas con cuatro tintes fluorescentes diferentes, que producen gránulos y cada unoMás información

Número de catálogo	Cantidad
T7281	0,5 mL

Número de catálogo T7281

Precio (MXN)

Cantidad:

0,5 mL

Las microesferas TetraSpec™ de 0,5 µm están completamente teñidas con cuatro tintes fluorescentes diferentes, que producen gránulos y cada uno de ellos muestra cuatro picos de excitación/emisión bien separados: 360/430 nm (azul), 505/515 nm (verde), 560/580 nm (naranja) y 660/680 nm (rojo oscuro). Estas microesferas pueden facilitar enormemente la calibración de microscopios de fluorescencia convencionales, microscopios de escaneo láser confocal y equipos de procesamiento de imagen asociados para la adquisición de imágenes científicas y comerciales, especialmente para aplicaciones multicolores.

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Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Tipo de calibraciónCalibración de microscopio confocal, calibración de microscopio de fluorescencia

FormatoGránulos de suspensión

Línea de productosTetraSpeck

Cantidad0,5 mL

Condiciones de envíoTemperatura ambiente

ColorNaranja, rojo oscuro, azul, verde, Dark Red, Blue, Green

Diámetro (métrico)0,5 μm

Tipo de productoMicroesfera

Unit Size0.5 mL

Contenido y almacenamiento

Almacenar en el refrigerador (de 2 °C a 8 °C) y proteger de la luz.

Preguntas frecuentes

What are the excitation/emission peaks for TetraSpeck Microspheres?

The TetraSpeck Microspheres (Cat. Nos. T7279, T7280, T7281, T7283, T7284, T14792) are stained throughout with four different fluorescent dyes, yielding beads that each display four well-separated excitation/emission peaks at 360/430 nm (blue), 505/515 nm (green), 560/580 nm (orange) and 660/680 nm (dark red).

TetraSpeck Blue Dye Spectra
Fluorescence excitation and emission spectra of bead encapsulated TetraSpeck blue dye.

TetraSpeck Orange Dye Spectra

TetraSpeck Green Dye Spectra

TetraSpeck Dark Red Dye Spectra
TetraSpeck Dark Red Spectra

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Citations & References (16)

Citations & References

Abstract

The cohesion protein ORD is required for homologue bias during meiotic recombination.

Authors:Webber HA, Howard L, Bickel SE

Journal:J Cell Biol

PubMed ID:15007062

'During meiosis, sister chromatid cohesion is required for normal levels of homologous recombination, although how cohesion regulates exchange is not understood. Null mutations in orientation disruptor (ord) ablate arm and centromeric cohesion during Drosophila meiosis and severely reduce homologous crossovers in mutant oocytes. We show that ORD protein localizes along ... More

Exocytosis of IgG as mediated by the receptor, FcRn: an analysis at the single-molecule level.

Authors:Ober RJ, Martinez C, Lai X, Zhou J, Ward ES

Journal:Proc Natl Acad Sci U S A

PubMed ID:15258288

'IgG transport within and across cells is essential for effective humoral immunity. Through a combination of biochemical and in vivo analyses, the MHC class I-related neonatal Fc receptor (FcRn) is known to play a central role in delivering IgGs within and across cells. However, little is known about the molecular ... More

Practical confocal microscopy and the evaluation of system performance.

Authors:Zucker RM, Price OT

Journal:Methods

PubMed ID:10491274

'The laser scanning confocal microscope has enormous potential in many fields of biology. Currently there is a subjective nature in the assessment of a confocal microscope''s performance by primarily evaluating the system with a specific test slide provided by the user''s laboratory. To achieve better performance from the equipment, it ... More

Mast cell degranulation requires N-ethylmaleimide-sensitive factor-mediated SNARE disassembly.

Authors:Puri N, Kruhlak MJ, Whiteheart SW, Roche PA

Journal:J Immunol

PubMed ID:14607937

'Mast cells possess specialized granules that, upon stimulation of surface FcR with IgE, fuse with the plasma membrane, thereby releasing inflammatory mediators. A family of membrane fusion proteins called SNAREs, which are present on both the granule and the plasma membrane, plays a role in the fusion of these granules ... More

Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.

Authors:Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A,

Journal:Cell

PubMed ID:18267078

The cell-cycle transition from G1 to S phase has been difficult to visualize. We have harnessed antiphase oscillating proteins that mark cell-cycle transitions in order to develop genetically encoded fluorescent probes for this purpose. These probes effectively label individual G1 phase nuclei red and those in S/G2/M phases green. We ... More

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