DAB Quanto Chromogen contains Diaminobenzidine (DAB) in buffer. DAB is a suspected carcinogen. Do not pipet by mouth. Avoid contact with skin and eyes. Reagent is acidic and can cause burns if skin contact occurs. Handle with care and dispose of according to regulations. DAB Quanto Substrate contains Hydrogen Peroxide in buffer. Avoid contact with skin and eyes.
1.Combine 30μL (1 drop) of DAB Quanto Chromogen with each 1 mL of DAB Quanto Substrate.
2. Apply mixture to tissue section.
3. Incubate tissue section for 5 minutes.
4. Rinse tissue, counterstain, dehydrate, clear in xylene or xylene substitute and coverslip.