Thermo Scientific™

Lab Vision™ UltraVision™ Large Volume Detection System: anti-Polyvalent, HRP (Ready-To-Use)

 Related applications:
Anatomical Pathology
Take advantage of a versatile and robust biotin-streptavidin detection system for research applications and cost-conscious laboratories with the Thermo Scientific™ Lab Vision™ UltraVision™ Large Volume Detection System: anti-Polyvalent, HRP (Ready-To-Use).

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Description

This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody that reacts with the primary antibody, enzyme-labeled streptavidin, and substrate/chromogen.
  • Labeled streptavidin-biotin immunoenzymatic antigen detection system
  • Species: Goat

  • Specificity: anti-Mouse IgG (H+L), anti-Rabbit IgG (H+L)

  • Enzyme: Peroxidase

  • Chromogen/Substrate: None provided

Includes:

TP-060-HL: 60mL Ultra V Block (TA-060-UB), 60mL Biotinylated Goat Anti-Polyvalent (TP-060-BN), 60mL Streptavidin Peroxidase (TS-060-HR)

TP-125-HL: 125mL Ultra V Block (TA-125-UB), 125mL Biotinylated Goat Anti-Polyvalent (TP-125-BN), 125mL Streptavidin Peroxidase (TS-125-HR)

Note:

UltraVision detection system detects a specific antibody bound to an antigen in tissue sections. The specific antibody is located by a biotin-conjugated secondary antibody. This step is followed by the addition of a streptavidin-enzyme conjugate that binds to the biotin present on the secondary antibody. The specific antibody, secondary antibody, and streptavidin-enzyme complex is then visualized with an appropriate substrate/chromogen.

Staining Protocol (kit components in bold):

1. Deparaffinize and rehydrate tissue section.

2. To reduce nonspecific background staining due to endogenous peroxidase, incubate slide in hydrogen peroxide for 10-15 minutes.

3. Wash 2 times in buffer.

4. If required, incubate tissue in digestive enzyme (or appropriate pretreatment).

5. Wash 4 times in buffer.

6. (Optional) Apply Ultra V Block and incubate for 5 minutes at room temperature to block nonspecific background staining.

NOTE: Do not exceed 10 minutes or there may be a reduction in desired stain.

7. Rinse (Optional).

8. Apply primary antibody and incubate according to manufacturer's protocol.

9. Wash 4 times in buffer.

10. Apply Biotinylated Goat Anti-Polyvalent and incubate for 10 minutes at room temperature.

11. Wash 4 times in buffer.

12. Apply Streptavidin Peroxidase and incubate for 10 minutes at room temperature.

13. Rinse 4 times in buffer.

14. Incubate with peroxidase-compatible chromogen of choice according to manufacturer's recommendations.

15. Counterstain and cover slip.

The specificity and sensitivity of antigen detection is dependent on the specific primary antibody used.



Documents

Manuals & protocols