Thermo Scientific™

BSTFA Silylation Reagent

 Related applications:
Industrial Chromatography

Improve GC analysis with Thermo Scientific™ BSTFA Silylation Reagent. The highly volatile byproducts mono(trimethylsilyl)trifluoroacetamide (BSTFA) and trifluoroacetamide elute with the solvent front to ensure excellent chromatographic separations. This silylation reagent reacts with a wide range of polar compounds and is particularly well suited for the GC analyses of some lower boiling TMS-amino acids and TMS Krebs cycle acids.

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Description

BSTFA is a powerful thrimethylsilyl donor, with donor strength that is comparable to its unfluorinated analog BSA [N,O-Bis(trimethylsilyl)acetamide]. BSTFA reacts to replace labile hydrogens on a wide range of polar compounds with a -Si(CH3)3 group. This physical characteristic is particularly useful in the gas chromatography of some lower boiling TMS-amino acids and TMS Krebs cycle acids.
  • Increased volatility of reaction byproducts mono(trimethylsilyl)trifluoroacetamide and trifluoroacetamide over corresponding nonfluorinated compounds from BSA
  • Increased volatility makes it possible to derivatize smaller molecules with which the TMS derivatives elute with the byproducts from BSA

Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.
General References:
  1. Her, G.R., et al. (1985). Anal. Biochem. 151, 292-298.
  2. Shanchun, J., et al. (1994). Org. Geochem. 21, 415-422.


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