Muller-Kauffmann Tetrathionate-Novobiocin (MkTTn) Enrichment Broth

Muller-Kauffmann Tetrathionate-Novobiocin Broth was developed by Muller² and later modified by Kauffmann^3,4 with the addition of ox bile and brilliant green to improve selectivity. The addition of novobiocin at 40 mg per liter was later described by Jeffries⁵ to improve inhibition of Proteus species.

After the sample has been incubated in Buffered Peptone Water (ISO), transfer 1 mL of the broth to 10 mL of MkTTn Broth and 0.1 mL into 10 mL of RVS Broth (TV5036E). Incubate the MkTTn Broth at 37°C ± 1°C for 24 hours ± 3 hours and the RVS Broth at 41.5°C ± 1°C, for 24 hours ± 3 hours. Sub-culture the incubated MkTTn and RVS broths onto XLD Agar (PO5057A/PO0164A) and a second agar medium of choice, and incubate for a further 24 hours ± 3 hours at 37°C ± 1°C. Presumptive Salmonella should be confirmed using appropriate biochemical and serological techniques. For the complete method, please refer to ISO 6579:2002¹.

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