Chromosome and replisome dynamics in E. coli: loss of sister cohesion triggers global chromosome movement and mediates chromosome segregation.
AuthorsBates D, Kleckner N
JournalCell
PubMed ID15960977
'Chromosome and replisome dynamics were examined in synchronized E. coli cells undergoing a eukaryotic-like cell cycle. Sister chromosomes remain tightly colocalized for much of S phase and then separate, in a single coordinate transition. Origin and terminus regions behave differently, as functionally independent domains. During separation, sister loci move far ... More
Characterizing peptide-mediated DNA internalization in human cancer cells.
AuthorsWittrup A, Belting M,
JournalMethods Mol Biol
PubMed ID19085116
'Cell penetrating peptides (CPPs) are currently used to deliver various macromolecular cargos to intracellular sites of action both in vitro and in vivo on an experimental basis. During the last few years, even more evidence has accumulated indicating that the main route of entry for most CPPs is through endocytosis ... More
A systemic small RNA signaling system in plants.
AuthorsYoo BC, Kragler F, Varkonyi-Gasic E, Haywood V, Archer-Evans S, Lee YM, Lough TJ, Lucas WJ
JournalPlant Cell
PubMed ID15258266
'Systemic translocation of RNA exerts non-cell-autonomous control over plant development and defense. Long-distance delivery of mRNA has been proven, but transport of small interfering RNA and microRNA remains to be demonstrated. Analyses performed on phloem sap collected from a range of plants identified populations of small RNA species. The dynamic ... More
Suspension array analysis of 16S rRNA from Fe- and SO(4)2- reducing bacteria in uranium-contaminated sediments undergoing bioremediation.
AuthorsChandler DP, Jarrell AE, Roden ER, Golova J, Chernov B, Schipma MJ, Peacock AD, Long PE
JournalAppl Environ Microbiol
PubMed ID16820459
'A 16S rRNA-targeted tunable bead array was developed and used in a retrospective analysis of metal- and sulfate-reducing bacteria in contaminated subsurface sediments undergoing in situ U(VI) bioremediation. Total RNA was extracted from subsurface sediments and interrogated directly, without a PCR step. Bead array validation studies with total RNA derived ... More
Ebola virus VP35-VP40 interaction is sufficient for packaging 3E-5E minigenome RNA into virus-like particles.
AuthorsJohnson RF, McCarthy SE, Godlewski PJ, Harty RN
JournalJ Virol
PubMed ID16698994
'The packaging of viral genomic RNA into nucleocapsids and subsequently into virions is not completely understood. Phosphoprotein (P) and nucleoprotein (NP) interactions link NP-RNA complexes with P-L (polymerase) complexes to form viral nucleocapsids. The nucleocapsid then interacts with the viral matrix protein, leading to specific packaging of the nucleocapsid into ... More
Role for centromeric heterochromatin and PML nuclear bodies in the cellular response to foreign DNA.
AuthorsBishop CL, Ramalho M, Nadkarni N, May Kong W, Higgins CF, Krauzewicz N,
JournalMol Cell Biol
PubMed ID16537904
Nuclear spatial positioning plays an important role in the epigenetic regulation of eukaryotic gene expression. Here we show a role for nuclear spatial positioning in regulating episomal transgenes that are delivered by virus-like particles (VLPs). VLPs mediate the delivery of plasmid DNA (pDNA) to cell nuclei but lack viral factors ... More
The clustering of four subfamilies of satellite DNA at individual chromosome ends in Silene latifolia.
AuthorsKazama Y, Sugiyama R, Suto Y, Uchida W, Kawano S
JournalGenome
PubMed ID16767177
The satellite DNA (satDNA) on the ends of chromosomes has been isolated and characterized in the dioecious plant Silene latifolia. BAC clones containing large numbers of repeat units of satDNA in a tandem array were isolated to examine the clustering of the repeat units. satDNA repeat units were purified from ... More
Dissecting the maize genome by using chromosome addition and radiation hybrid lines.
We have developed from crosses of oat (Avena sativa L.) and maize (Zea mays L.) 50 fertile lines that are disomic additions of individual maize chromosomes 1-9 and chromosome 10 as a short-arm telosome. The whole chromosome 10 addition is available only in haploid oat background. Most of the maize ... More