ULYSIS™ Alexa Fluor™ 647 Nucleic Acid Labeling Kit, 650/670 nm - FAQs

View additional product information for ULYSIS™ Alexa Fluor™ Nucleic Acid Labeling Kit - FAQs (U21654, U21652, U21650, U21660)

9 product FAQs found

Is ULYSIS labeling compatible with microarray analysis?

Yes, there are numerous examples of ULYSIS labeled probes that have been used in microarray analysis. Here are a few publications for your reference:

- Babak T, Zhang W, Marros Q et al. (2004) Probing microRNAs with microarrays: tissue specificity and functional inference. RNA 10(11):1813-1819.
- Hiley SL, Jackman J, Babak T et al. (2005) Detection and discovery of RNA modifications using microarrays. Nucleic Acids Res 33(1):e2.
- Torchet C, Badis G, Devaux F et al. (2005) The complete set of H/ACA snoRNAs that guide rRNA pseudouridylations in Saccharomyces cerevisiae. RNA 11(6):928-938.

Can probes labeled with the ULYSIS Nucleic Acid Labeling Kits be stored for later use?

Long-term storage for the ULYSIS labeled probes can be done in just about any kind of buffer, TE, formamide, hybridization buffer, or ethanol. We suggest using your normal storage conditions as long as you protect the probes from light. ULYSIS conjugates are very stable. Avoid phenol.

Do you have any tips on using the ULYSIS Nucleic Acid Labeling Kits for RNA labeling?

A preliminary protocol modifies our DNA-labeling protocol: Do not nuclease-treat the RNA, but label it directly by incubating for 10 minutes at 90°C or 15 minutes at 85°C. Add 2 µg of glycogen for every 1 µg of RNA and purify by ethanol precipitation. Refer to these publications:

- Babak T, Zhang W, Marros Q et al. (2004) Probing microRNAs with microarrays: tissue specificity and functional inference. RNA 10(11):1813-1819.
- Hiley SL, Jackman J, Babak T et al. (2005) Detection and discovery of RNA modifications using microarrays. Nucleic Acids Res 33(1):e2.
- Torchet C, Badis G, Devaux F et al. (2005) The complete set of H/ACA snoRNAs that guide rRNA pseudouridylations in Saccharomyces cerevisiae. RNA 11(6):928-938.

Can the ULYSIS kits be used on probes longer than 1,000 base pairs or even plasmids?

It might be possible to label larger probes with the ULYSIS Nucleic Acid Labeling Kits, but the dye will likely need to be diluted to avoid (or at least reduce) problems with aggregation. Refer to this publication: Coelho-Castelo AA, Santos Junior RR, Bonato VL et al. (2003) B-lymphocytes in bone marrow or lymph nodes can take up plasmid DNA after intramuscular delivery. Hum Gene Ther 14(13):1279-1285.

How stable is the ULYSIS labeled DNA to high temperature?

An oligonucleotide labeled with a ULYSIS Nucleic Acid Labeling Kit should survive 100°C for 5 minutes, and storage at 68°C overnight should also not cause any dissociation of the complex.

My recovery of DNA after labeling with ULYSIS Nucleic Acid Labeling Kit, and precipitation is low. What can I do to improve it?

After labeling and purifying your DNA, you can add 10 µg of salmon sperm DNA. This should improve your recovery after precipitation.

Can I use the DNA labeled using ULYSIS Nucleic Acid Labeling Kit, for hybridization directly after column purification?

We recommend using ethanol precipitation to concentrate the sample first to calculate the DNA concentration and degree of labeling.

Could you provide an overview of the ULYSIS Nucleic Acid Labeling Kits?

The ULYSIS oligonucleotide chemical labeling reagents allow you to rapidly and easily couple our fluorescent dyes to purine bases in nucleic acid polymers. The method, the Universal Linkage System (ULS), is based on the use of a platinum coordination complex that forms a stable adduct at the N7 position of guanine. ULYSIS labeled probes are used in fluorescence in situ hybridization (FISH) and numerous other applications.

What are the ULYSIS labeling kits used for?

ULYSIS labeling kits are used for labeling short, unmodified DNA and RNA strands between ~100-1,000 bp for FISH analysis.

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