Thermo Scientific™

UltiMate™ 3000 RSLCnano System

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Industrial & Applied Science

Get outstanding performance and workflow versatility using the Thermo Scientific™ UltiMate™ 3000 RSLCnano system. This low-flow liquid chromatography system has a wide flow-pressure footprint with nano-, capillary-, and micro-flow options. Our advanced Thermo Scientific™ ProFlow™ technology improves nano-flow rate control and results in high retention time precision and gradient reproducibility.

Achieve high-sensitivity and excellent separation with our nano-flow solution, or balance sensitivity and high-throughput with standardized, robust capillary- and micro-flow solutions.

Pre-concentrate your sample on a trapping column with an integrated micro-flow loading pump, set up advanced tandem and two-dimensional workflows for maximum MS utilization and productivity or tailor it to your needs with our dedicated application kits.

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Outstanding Performance and Workflow Versatility

The wide flow range, high-pressure binary gradient pump, the additional micro-flow ternary gradient pump, and integrated column compartment with two snap-in switching valves makes the UltiMate 3000 RSLCnano suitable for virtually any low-flow LC or LCMS application such as bottom-up and top-down proteomics, or metabolomics. Use dedicated application kits to easily setup standard and advanced workflow solutions.

Outstanding Performance

  • ProFlow technology for nano-flow applications provides the highest level of retention time precision and gradient reproducibility
  • Capillary-flow flow meter enables high-sensitivity with high-throughput analyses for reliable routine assays
  • Continuous-flow binary, high-pressure gradient pump provides uninterrupted gradient delivery permitting ultra-long, reproducible gradients
  • Integrated micro-flow ternary gradient pump provides uninterrupted continuous flow through the trap columns, even during sample loading, making sample pre-concentration and two-dimensional workflows possible
  • Ultra-high pressure capability of up to 860 bar on the column for UHPLC separations

Impressive Versatility

  • Wide flow rate range with active flow control from 50 nL/min to 50 µL/min
  • Integrated micro-flow pump for flow rates up to 2500 µL/min for quick sample loading, desalting and pre-concentration onto trap columns and for use in advanced applications
  • Dedicated application kits for standard and advanced applications, such as direct injection, pre-concentration, tandem low-flow LC, or two-dimensional workflows

Easy Connections for Better Chromatography

Thermo Scientific™ nanoViper™ Fingertight Fittings make all your UHPLC and HPLC capillary connections fast, easy and reliable. nanoViper fingertight fittings require no tools, are reusable and designed to seal at the tip to minimize dead volume between connections for higher efficiency and better chromatography with maximum separation quality.

Seamless LC-MS Integration

  • Dedicated connection kits and LC-MS interfaces for straightforward hardware integration with Thermo Scientific™ EASY-Spray™, Thermo Scientific™ Nanospray Flex™, and Thermo Scientific™ Ion Max sources
  • Seamless software integration with Thermo Scientific mass spectrometers via Thermo Scientific™ Standard Instrument Integration (SII) for Xcalibur™ and single-point of intelligent control with the easy-to-use Thermo Scientific™ Chromeleon™ Chromatography Data System software