pcDNA™6/TR vector Mammalian Expression Vector

Catalog number: V102520

Invitrogen™  Related applications: Protein Expression

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Description

The pcDNA™6⁄TR vector is part of the T-REx™ System (catalog K102001), which yields higher levels of induced expression than any other regulated mammalian expression system. It utilizes the complete CMV promoter and adds control elements from the bacterial tetracycline resistance operon to effectively repress and derepress transcription from one of the strongest mammalian promoter sequences known (1,2).

The regulatory vector, pcDNA™6⁄TR, is provided for high-level expression of the tetracycline repressor (TR) protein. This vector expresses the Blasticidin resistance gene for rapid selection of mammalian cell lines that stably express the TR protein.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: CMV
Key Function: Regulated Expression
Protein Tag or Fusion: Untagged
Delivery Method: Transfection
Selection Agent (Eukaryotic): Blasticidin
Vector Type: pcDNA
Product Size: 20 µg
Shipping Condition: Room Temperature
Constitutive or Inducible System: Inducible

Contents & storage

20 µg of supercoiled vector (40 µL at 0.5 µg/µL) supplied in TE Buffer, pH 8.0. Store at -20°C.

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Manuals & protocols

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