pcDNA™5/TO Mammalian Expression Vector

Catalog number:  V103320

 Related applications:

Protein Expression

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pcDNA™5/TO is a 5.7 kb expression vector designed for use with the T-REx™ System (Catalog nos. K1020-01 and K1020-02). The T-REx™ System yields higher levels of induced expression than any other regulated mammalian expression system. It utilizes the complete CMV promoter and adds control elements from the bacterial tetracycline resistance operon to effectively repress and derepress transcription from one of the strongest mammalian promoter sequences known.

The pcDNA™5/TO vector allows tetracycline-regulated expression of the gene of interest in mammalian host cells expressing the Tet repressor (TetR) from the pcDNA™6/TR vector (Catalog no. V1025-20).


Promoter: CMV⁄TO
Key Function: Regulated Expression
Protein Tag or Fusion: Untagged
Delivery Method: Transfection
Cloning Method: Restriction Enzyme ⁄ MCS
Selection Agent (Eukaryotic): Hygromycin
Vector Type: pcDNA
Constitutive or Inducible System: Inducible
Inducing Agent: Tetracycline
Product Line: T-REx™, pcDNA™
Product Size: 20 µg
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Contents & storage

Store pcDNA™5/TO at -20°C


Manuals & protocols