Invitrogen™

Single-Channel Dead Cell Apoptosis Kit with Annexin V Alexa Fluor™ 488 and SYTOX™ Green Dyes, for flow cytometry

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Description

This product detects the externalization of phosphatidylserine in apoptotic cells using recombinant annexin V conjugated to green-fluorescent Alexa Fluor™ 488 dye and dead cells using SYTOX™ Green nucleic acid stain. After treatment with both probes, apoptotic cells show green fluorescence, dead cells show brighter green fluorescence, and live cells show little or no fluorescence. These populations can easily be distinguished in the FL1 channel of a flow cytometer, freeing the other channels for detection of other fluorescent colors.

View a selection guide for all apoptosis assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Excitation/Emission: SYTOX® green: 503⁄524, Alexa Fluor® 488: 499⁄521
Flow Cytometer Laser Lines: 488
Label Type: Alexa Fluor Dyes, Other Label(s) or Dye(s)
Label or Dye: Alexa Fluor™ 488, SYTOX™ Green
No. of Reactions: 50
Product Line: Alexa Fluor™, SYTOX®
Shipping Condition: Wet Ice
Detection Method: Fluorescent
For Use With (Equipment): Flow Cytometer
Format: Tube(s)

Contents & storage

Contains 1 vial of annexin V, Alexa Fluor® 488 conjugate (250 µL), 1 vial of SYTOX® green (100 µL), and 1 bottle of annexin binding buffer (5X solution, 50 mL).

Store in refrigerator (2–8°C) and protect from light.