Dead Cell Apoptosis Kit with Annexin V Alexa Fluor™ 488 & Propidium Iodide (PI)

Catalog number: V13241

Related applications:

Cell Viability, Proliferation & Function

Description

The Dead Cell Apoptosis Kit with Annexin V Alexa Fluor™ 488 & Propidium Iodide is a flow cytometry kit used to measure early apoptosis by detecting phosphatidyl serine expression and membrane permeability.

View a selection guide for all apoptosis assays for flow cytometry.

Superior brightness
Unlike other Annexin V kits that have lower protein concentrations or purification levels, the Annexin V Alexa Fluor™ 488 conjugate is optimized for flow cytometry to provide the largest separation between apoptotic and live cells. The Alexa Fluor™ 488 dye is a superior green-fluorescent dye with a spectrum similar to fluorescein (FITC).

High binding efficiency
Annexin V conjugates are made from a highly purified cys-annexin, which leads to higher binding efficiency, resulting in highly accurate characterization of the apoptotic process.

Multi-parametric
Many publications require a minimum of two different ways to identify that cells are apoptotic. This multi-parametric kit detects phosphatidyl serine (PS) on the cytoplasmic surface of the cell membrane and membrane integrity using propidium iodide.

How it works
When cells are stained with Annexin V and propidium iodide, apoptotic cells expressing PS show green fluorescence, which can be detected in the FITC channel, and low red fluorescence. Dead or necrotic cells show bright red fluorescence and no green fluorescence, while live cells show no green or red fluorescence.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Excitation/Emission:	Alexa Fluor® 488: 499⁄521, PI: 535⁄617
Flow Cytometer Laser Lines:	488
For Use With (Equipment):	Fluorescence Microscope, Flow Cytometer
Label or Dye:	Alexa Fluor™ 488, propidium iodide
No. of Reactions:	50
Product Line:	Alexa Fluor™
Shipping Condition:	Wet Ice
Quantity:	50 Assay(s)
Format:	Tube(s), Slide(s)
Detection Method:	Fluorescent

Contents & storage

Contains 1 vial of annexin V, Alexa Fluor® 488 conjugate (250 µL), 1 vial of propidium iodide (PI, 100 µL), and 1 bottle of annexin binding buffer (5X solution, 15 mL).

Store in refrigerator (2–8°C) and protect from light.