This.Is.Simple. Reduce assay times with the CellEvent® Kit for flow cytometry
Invitrogen™
Dead Cell Apoptosis Kit with Annexin V Alexa Fluor™ 488 & Propidium Iodide (PI)
Error loading your content!
| Catalog number |
Select a plan
Unit size
|
Price ({{currency}}) | Availability | Qty | |
|---|---|---|---|---|---|
| {{product.sku}} {{product.sku}} also known as {{product.formattedSku}} |
|
— |
Pro add-ons
Your on-site stock
››
{{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
››
{{supplyCenter.scName}}(Out of stock)
››
{{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Description
The Dead Cell Apoptosis Kit with Annexin V Alexa Fluor™ 488 & Propidium Iodide is a flow cytometry kit used to measure early apoptosis by detecting phosphatidyl serine expression and membrane permeability.View a selection guide for all apoptosis assays for flow cytometry.
Superior brightness
Unlike other Annexin V kits that have lower protein concentrations or purification levels, the Annexin V Alexa Fluor™ 488 conjugate is optimized for flow cytometry to provide the largest separation between apoptotic and live cells. The Alexa Fluor™ 488 dye is a superior green-fluorescent dye with a spectrum similar to fluorescein (FITC).
High binding efficiency
Annexin V conjugates are made from a highly purified cys-annexin, which leads to higher binding efficiency, resulting in highly accurate characterization of the apoptotic process.
Multi-parametric
Many publications require a minimum of two different ways to identify that cells are apoptotic. This multi-parametric kit detects phosphatidyl serine (PS) on the cytoplasmic surface of the cell membrane and membrane integrity using propidium iodide.
How it works
When cells are stained with Annexin V and propidium iodide, apoptotic cells expressing PS show green fluorescence, which can be detected in the FITC channel, and low red fluorescence. Dead or necrotic cells show bright red fluorescence and no green fluorescence, while live cells show no green or red fluorescence.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Flow Cytometer Laser Lines: | 488 |
|---|---|
| Detection Method: | Fluorescent |
| Excitation⁄Emission (nm): | Alexa Fluor® 488: 499⁄521, PI: 535⁄617 |
| For Use With (Equipment): | Flow Cytometer, Fluorescence Microscope |
| Format: | Slide(s), Tube(s) |
| Label or Dye: | Alexa Fluor™ 488, propidium iodide |
| Number of Reactions: | 50 |
| Product Line: | Alexa Fluor™ |
| Product Size: | 50 assays |
| Shipping Condition: | Wet Ice |
Contents & storage
Contains 1 vial of annexin V, Alexa Fluor® 488 conjugate (250 µL), 1 vial of propidium iodide (PI, 100 µL), and 1 bottle of annexin binding buffer (5X solution, 15 mL).
Store in refrigerator (2–8°C) and protect from light.
Store in refrigerator (2–8°C) and protect from light.
