pPIC6 A, B, & C Pichia Vectors

Catalog number: V21020

Invitrogen™  Related applications: Protein Expression

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pPIC6 A, B, & C are Pichia pastoris expression vectors designed for simple selection,
high-level expression, and rapid protein purification and detection. The pPIC6 A,
B, & C vectors are designed for high-level secreted expression. Both sets of vectors
have the following features:
Blasticidin resistance for direct selection of multi-copy integrants
Inducible AOX1 promoter for high-level expression in Pichia pastoris
C-terminal c-myc epitope for convenient detection with an Anti-myc Antibody
C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating
resin and detection with an Anti-His(C-term) Antibody
pPIC6 also features:
An É -factor secretion signal for efficient transport of proteins to the medium
For Research Use Only. Not for use in diagnostic procedures.



Promoter: AOX1
Vector Type: pPIC
Product Size: 20 µg
Cloning Method: Restriction Enzyme ⁄ MCS
Protein Tag or Fusion: His Tag (6x), c-Myc Epitope Tag
Antibiotic Resistance (Bacterial): Blasticidin (BsdR)

Contents & storage

Each vector kit includes 20 µg each of expression vector in A, B, & C versions, 20 µg of a lacZ (intracellular) or HSA (secreted) control vector, and an X-33 Pichia stab. Store vectors at -20°C. Store the Pichia stab at +4°C. All reagents are guaranteed stable for 6 months when properly stored.


Manuals & protocols