CyQUANT™ Cytotoxicity Assay Kit (G6PD Release Assay)

Citas y referencias (7)

Invitrogen™

CyQUANT™ Cytotoxicity Assay Kit (G6PD Release Assay)

El ensayo de citotoxicidad CyQUANT detecta células dañadas y moribundos mediante su liberación de glucosa 6-fosfato deshidrogenasa en el medioMás información

Número de catálogo	Cantidad
V23111	1000 Assays

Número de catálogo V23111

Precio (MXN)

Cantidad:

1000 Assays

El ensayo de citotoxicidad CyQUANT detecta células dañadas y moribundos mediante su liberación de glucosa 6-fosfato deshidrogenasa en el medio circundante. Puede detectar hasta 500 células y es más sensible que los ensayos de liberación de LDH.

El ensayo detecta la glucosa 6-fosfato deshidrogenasa mediante un proceso enzimático de dos pasos que conduce a la reducción de la resazurina en resorufina roja fluorescente. La señal de fluorescencia resultante es proporcional a la cantidad de glucosa 6-fosfato deshidrogenasa que se libera en el medio celular, y esta liberación se correlaciona con el número de células muertas de la muestra. La emisión de fluorescencia de resorufina (ex/em 563/587 nm) supera la autofluorescencia de la mayoría de las muestras biológicas. Además, este ensayo produce señales de fondo más bajo a las que se observan normalmente en ensayos basados en la lactosa deshidrogenasa.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Permeabilidad celularCell-impermeant

Método de detecciónFluorescence

Tipo de coloranteOtras etiquetas o colorantes

FormatoPlaca de 96 pocillos

Cantidad1000 Assays

Condiciones de envíoTemperatura ambiente

ColorRed

Emission587 nm

Excitation Wavelength Range563 nm

Para utilizar con (aplicación)Kit de ensayo de citotoxicidad (ensayo de liberación G6PD)

Para utilizar con (equipo)Lector de microplacas

Línea de productosCyQUANT

Tipo de productoG6PD Release Cytotoxicity Assay

Unit Size1 kit

Contenido y almacenamiento

Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

What are the fluorescence excitation/emission maxima for Resorufin that is generated in the Vybrant Cytotoxicity Assay Kit (G6PD Release Assay)?

The excitation/emission maxima for Resorufin are 563/587 nm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How many assays can I perform using the CyQUANT Cytotoxicity Assay Kit (G6PD Release Assay) (Cat. No. V23111)?

The CyQUANT Cytotoxicity Assay Kit (G6PD Release Assay) (Cat. No. V23111) contains sufficient reagents to perform 1,000 assays using 100 µL reaction volumes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use flash-frozen supernatant from the cell culture for the CyQUANT Cytotoxicity Assay (Cat. No. V23111)?

Using flash‐frozen supernatant is not recommended for the CyQUANT Cytotoxicity Assay. It is better to take the reading straight after addition of the cytotoxic agent followed by an incubation and addition of the 2X Resazurin/Reaction Mixture.

Find additional tips, troubleshooting help, and resources within our Cell Viability, Proliferation, Cryopreservation, and Apoptosis Support Center

Citations & References (7)

Citations & References

Abstract

Use of cellular glucose-6-phosphate dehydrogenase for cell quantitation: applications in cytotoxicity and apoptosis assays.

Authors:Batchelor RH, Zhou M

Journal:Anal Biochem

PubMed ID:15136165

'A fluorescence-based microplate assay was developed to quantify cell death based upon the measurement of glucose-6-phosphate dehydrogenase (G6PD) activity. G6PD is a cytosolic enzyme and leaks from cells when plasma membrane integrity is compromised. In this assay, cell death is measured by correlating the activity of extracellular G6PD to the ... More

Development of a comprehensive human immunodeficiency virus type 1 screening algorithm for discovery and preclinical testing of topical microbicides.

Authors:Lackman-Smith C, Osterling C, Luckenbaugh K, Mankowski M, Snyder B, Lewis G, Paull J, Profy A, Ptak RG, Buckheit RW, Watson KM, Cummins JE, Sanders-Beer BE,

Journal:Antimicrob Agents Chemother

PubMed ID:18316528

'Topical microbicides are self-administered, prophylactic products for protection against sexually transmitted pathogens. A large number of compounds with known anti-human immunodeficiency virus type 1 (HIV-1) inhibitory activity have been proposed as candidate topical microbicides. To identify potential leads, an in vitro screening algorithm was developed to evaluate candidate microbicides in ... More

In vivo imaging platform for tracking immunotherapeutic cells.

Authors:Ahrens ET, Flores R, Xu H, Morel PA

Journal:Nat Biotechnol

PubMed ID:16041364

Cellular therapeutics show great promise for the treatment of disease, but few noninvasive techniques exist for monitoring the cells after administration. Here we present a magnetic resonance imaging (MRI) technology that uses perfluoropolyether (PFPE) agents to track cells in vivo. Fluorine MRI selectively images only the labeled cells, and a ... More

Kaposi's sarcoma-associated herpesvirus induces rapid release of angiopoietin-2 from endothelial cells.

Authors:Ye FC, Zhou FC, Nithianantham S, Chandran B, Yu XL, Weinberg A, Gao SJ,

Journal:J Virol

PubMed ID:23536671

Kaposi sarcoma-associated herpesvirus (KSHV) stimulates proliferation, angiogenesis, and inflammation to promote Kaposi sarcoma (KS) tumor growth, which involves various growth factors and cytokines. Previously, we found that KSHV infection of human umbilical vein endothelial cells (HUVECs) induces a transcriptional induction of the proangiogenic and proinflammatory cytokine angiopoietin-2 (Ang-2). Here, we ... More

Extracellular DNA, Neutrophil Extracellular Traps, and Inflammasome Activation in Severe Asthma.

Authors:

Journal:Am J Respir Crit Care Med

PubMed ID:30888839

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