The CyQUANT Cytotoxicity Assay detects damaged and dying cells by means of their release of glucose 6-phosphate dehydrogenase into the surrounding medium. It can detect as few as 500 cells and is more sensitive than LDH release assays.
The assay detects glucose 6-phosphate dehydrogenase through a two-step enzymatic process that leads to the reduction of resazurin into red-fluorescent resorufin. The resulting fluorescence signal is proportional to the amount of glucose 6-phosphate dehydrogenase released into the cell medium, and this release correlates with the number of dead cells in the sample. The fluorescence emission of resorufin (ex/em 563/587 nm) is beyond the autofluorescence of most biological samples. In addition, this assay results in lower background signals than are typically observed in lactate dehydrogenase-based assays.
For Research Use Only. Not for use in diagnostic procedures.
For Use With (Application)
Cytotoxicity Assay Kit (G6PD Release Assay)
For Use With (Equipment)
Other Label(s) or Dye(s)
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.