This.Is.Simple. Reduce assay times with the CellEvent® Kit for flow cytometry

      Vybrant™ Apoptosis Assay Kit #6, Biotin-X Annexin V/Alexa Fluor™ 350 Streptavidin/Propidium Iodide

      Vybrant™ Apoptosis Assay Kit #6, Biotin-X Annexin V/Alexa Fluor™ 350 Streptavidin/Propidium Iodide

      Catalog number:  V23200

      Invitrogen™  Related applications: Cell Viability, Proliferation & Function | Cellular Imaging | Chromatin Biology | Flow Cytometry | Pharma & Biopharma | Target-Based ADME⁄Tox Assays

        Contact us for support ›

      Error loading your content!

        Catalog number
      Select a plan
      Unit size
      		 Price ({{currency}}) Availability Qty
      {{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
      		{{product.availability.message}} —
      Pro add-ons
      Customize this product ›

      Your on-site stock

      ›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
      ›› {{supplyCenter.scName}}(Out of stock)
      ›› {{supplyCenter.scName}}
      This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
      Change selected location › Order non-stocked items
      Back to top

      Description

      The Vybrant Apoptosis Assay Kit #6 uses the bright, blue-fluorescent Alexa Fluor 350 conjugate of streptavidin in conjunction with biotin-X annexin V to detect apoptotic cell populations either by flow cytometry or imaging. Alexa Fluor 350 streptavidin binds to biotin-X annexin V, which in turn binds to the phosphatidylserine on the surfaces of apoptotic cells. Necrotic cells are distinguished using red-fluorescent propidium iodide, which binds tightly to the nucleic acids in those cells. Propidium iodide does not penetrate the plasma membranes of live cells or early apoptotic cells. The kit contains an ample amount of each reagent for about 50 flow cytometric assays.

      View a selection guide for all apoptosis assays for flow cytometry.
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Detection Method: Fluorescent
      Excitation⁄Emission (nm): Alexa Fluor® 350: 346⁄442, PI: 535⁄617
      For Use With (Equipment): Flow Cytometer
      Form: Solution
      Format: Tube(s)
      Label or Dye: Alexa Fluor™ 350, Biotin-X, propidium iodide
      Number of Reactions: 50
      Product Line: Alexa Fluor™
      Product Size: 50 assays
      Shipping Condition: Wet Ice

      Contents & storage

      Contains 1 vial of biotin-x anneixn V, 1 vial of Alexa Fluor® 350 streptavidin, 1 vial of propidium iodide, and 1 bottle of 5x annexin binding buffer.

      Store in refrigerator (2–8°C) and protect from light.

      Documents

      Manuals & protocols

      Recommended products