Invitrogen™

Vybrant™ Apoptosis Assay Kit #6, Biotin-X Annexin V/Alexa Fluor™ 350 Streptavidin/Propidium Iodide

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Description

The Vybrant Apoptosis Assay Kit #6 uses the bright, blue-fluorescent Alexa Fluor 350 conjugate of streptavidin in conjunction with biotin-X annexin V to detect apoptotic cell populations either by flow cytometry or imaging. Alexa Fluor 350 streptavidin binds to biotin-X annexin V, which in turn binds to the phosphatidylserine on the surfaces of apoptotic cells. Necrotic cells are distinguished using red-fluorescent propidium iodide, which binds tightly to the nucleic acids in those cells. Propidium iodide does not penetrate the plasma membranes of live cells or early apoptotic cells. The kit contains an ample amount of each reagent for about 50 flow cytometric assays.

View a selection guide for all apoptosis assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Excitation/Emission: Alexa Fluor® 350: 346⁄442, PI: 535⁄617
Flow Cytometer Laser Lines: UV, 488
Form: Solution
Label Type: Alexa Fluor Dyes, Biotin & Other Haptens, Other Label(s) or Dye(s)
Label or Dye: Alexa Fluor™ 350, propidium iodide, Biotin-X
No. of Reactions: 50
Product Line: Alexa Fluor™
Shipping Condition: Wet Ice
Detection Method: Fluorescent
For Use With (Equipment): Flow Cytometer
Format: Tube(s)

Contents & storage

Contains 1 vial of biotin-x anneixn V, 1 vial of Alexa Fluor® 350 streptavidin, 1 vial of propidium iodide, and 1 bottle of 5x annexin binding buffer.

Store in refrigerator (2–8°C) and protect from light.