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Citas y referencias (11)
Invitrogen™
Vybrant™ Alexa Fluor™ 594 Lipid Raft Labeling Kit
Los kits de etiquetado de balsas lipídicas Vybrant están diseñados para proporcionar un etiquetado fluorescente cómodo, fiable y extremadamente brillanteMás información
| Número de catálogo | Cantidad |
|---|---|
| V34405 | 1 kit |
Número de catálogo V34405
Precio (MXN)
-
Cantidad:
1 kit
Los kits de etiquetado de balsas lipídicas Vybrant están diseñados para proporcionar un etiquetado fluorescente cómodo, fiable y extremadamente brillante de balsas lipídicas en células vivas. Los kits incluyen la subunidad B (CT-B) de la toxina del cólera marcada con los colorantes verde fluorescente Alexa Fluor 488, el naranja fluorescente Alexa Fluor 555 o el rojo fluorescente Alexa Fluor 594, así como un anticuerpo que reconoce específicamente CT-B. Los kits también incluyen una solución 10X de solución salina tamponada con fosfato (10X PBS), pH 7,2 e instrucciones detalladas.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Línea de productosAlexa Fluor
Subtipo de proteínaLa toxina del cólera
Cantidad1 kit
Condiciones de envíoHielo húmedo
Tipo de etiquetaColorantes Alexa Fluor
Tipo de productoKit de etiquetado de grandes cantidades de lípidos
SubCellular LocalizationCell Membranes and Lipids
Unit Size1 kit
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.
Citations & References (11)
Citations & References
Abstract
Looking at lipid rafts?
Journal:Trends Cell Biol
PubMed ID:10201072
'The notion that microdomains enriched in certain specialized lipids exist in membranes has been both attractive and controversial since it was first proposed that such domains, termed rafts, might act as apical sorting devices in epithelial cells. The observation that certain lipids are not extractable in cold nonionic detergent supports
Functions of lipid rafts in biological membranes.
Journal:Annu Rev Cell Dev Biol
PubMed ID:9891780
'Recent studies showing that detergent-resistant membrane fragments can be isolated from cells suggest that biological membranes are not always in a liquid-crystalline phase. Instead, sphingolipid and cholesterol-rich membranes such as plasma membranes appear to exist, at least partially, in the liquid-ordered phase or a phase with similar properties. Sphingolipid and
Lipid rafts make for slippery platforms.
Journal:J Cell Biol
PubMed ID:12885764
What's in a raft? Although cell membranes are certainly not homogeneous mixtures of lipids and proteins, almost all aspects of lipid rafts-how to define them, their size, composition, lifetime, and biological relevance-remain controversial. The answers will shape our views of signaling and of membrane dynamics.
Lipid rafts and little caves. Compartmentalized signalling in membrane microdomains.
Journal:Eur J Biochem
PubMed ID:11846775
Lipid rafts are liquid-ordered membrane microdomains with a unique protein and lipid composition found on the plasma membrane of most, if not all, mammalian cells. A large number of signalling molecules are concentrated within rafts, which have been proposed to function as signalling centres capable of facilitating efficient and specific
Elevated NADPH oxidase activity contributes to oxidative stress and cell death in Huntington's disease.
Journal:Hum Mol Genet
PubMed ID:23223017
A mutation in the huntingtin (Htt) gene produces mutant Htt and Huntington's disease (HD), a neurodegenerative disorder. HD patients have oxidative damage in the brain, but the causes are unclear. Compared with controls, we found brain levels of NADPH oxidase (NOX) activity, which produces reactive oxygen species (ROS), elevated in