Invitrogen™

Vybrant™ DyeCycle™ Orange Stain

Catalog number: V35005
Invitrogen™

Vybrant™ DyeCycle™ Orange Stain

Catalog number: V35005
Catalog Number
V35005
Unit Size
400 µL
Price (USD)
457.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
V35005400 µL
457.00
Your Price:
***
Product Overview
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Vybrant® DyeCycle™ Orange Stain is a cell permeable DNA dye that can be used for cell cycle analysis on 488 nm or 532 nm laser lines.

• Precise—accurate cell cycle analysis in living cells

• Safe—low cytotoxicity for cell sorting and additional live cell experiments

• Simple, robust staining protocol

View a selection guide for all products related to cell cycle analysis of fixed and live cells in flow cytometry.

Precise

Successful cell cycle analysis requires a dye that is DNA selective and can stain cells in a homogeneous pattern minimizing fluorescence variability. The Vybrant® DyeCycle™ Orange Stain is an ideal tool for DNA content analysis in living cells since the stains are cell permeable and, after binding double-stranded DNA, emit a fluorescent signal that is proportional to the DNA mass (see figure).

Low Cytotoxicity

Unlike stains that require high concentrations or have chemical structures that are toxic to cells, the Vybrant® DyeCycle™ Orange Stain exhibits relatively low cytotoxicity, allowing the possibility of sorting based on the phase of the cell cycle.

Simple, Robust Staining Protocol

For cell analysis, simply prepare flow cytometry tubes containing 1 mL of cells suspended in complete media at a concentration of 1 × 106 cells/mL. To each tube add 2 µL of Vybrant® DyeCycle™ Orange Stain. Final stain concentration is 10 µM. Incubate at 37°C for 30 minutes, protected from light. Analyze samples on flow cytometer using 488 nm excitation and orange emission.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
5 mM
Detection Method
Fluorescent
Dye Type
Vybrant™ DyeCycle™ Orange
Emission
532, 488
Form
Solution
Format
Tube(s)
Product Line
DyeCycle™
Quantity
200
Shipping Condition
Room Temperature
Solubility
Water
Sub Cellular Localization
Nucleic Acids
For Use With (Equipment)
Flow Cytometer

Contents & Storage

Contains 1 vial of Vybrant™ DyeCycle™ orange stain (5 mM in DMSO). Store at ≤-20°C, desiccate and protect from light.

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Citations & References