Invitrogen™

Dead Cell Apoptosis Kit with Annexin V PE and SYTOX™ Green, for flow cytometry

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Description

This product detects the externalization of phosphatidylserine in apoptotic cells using recombinant annexin V conjugated to the orange fluorescent phycobiliprotein R-PE, and dead cells using SYTOX™ Green nucleic acid stain. After treatment with both probes, apoptotic cells show orange fluorescence, dead cells show green fluorescence, and live cells show little or no fluorescence. These populations can easily be distinguished in the 530/30 nm and 585/42 nm bandpass filters with a 488 nm laser flow cytometer.

View a selection guide for all apoptosis assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Excitation/Emission: SYTOX® green: 503⁄524, R-PE: 488⁄575
Flow Cytometer Laser Lines: 561, 488
For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
Label or Dye: R-PE, SYTOX™ Green
No. of Reactions: 50
Product Line: SYTOX®
Shipping Condition: Wet Ice
Format: Tube(s), Slide(s)
Detection Method: Fluorescent

Contents & storage

Contains 1 vial of annexin V, R-PE conjugate (250 µL), 1 vial of SYTOX® green stain (100 µL), and 1 bottle of annexin binding buffer (5X solution, 15 mL).

Store in refrigerator (2–8°C) and protect from light.