Dead Cell Apoptosis Kit with Annexin V PE and SYTOX™ Green, for flow cytometry

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This product detects the externalization of phosphatidylserine in apoptotic cells using recombinant annexin V conjugated to the orange fluorescent phycobiliprotein R-PE, and dead cells using SYTOX™ Green nucleic acid stain. After treatment with both probes, apoptotic cells show orange fluorescence, dead cells show green fluorescence, and live cells show little or no fluorescence. These populations can easily be distinguished in the 530/30 nm and 585/42 nm bandpass filters with a 488 nm laser flow cytometer.

View a selection guide for all apoptosis assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.


Flow Cytometer Laser Lines: 488, 561
Detection Method: Fluorescent
Excitation⁄Emission (nm): R-PE: 488⁄575, SYTOX® green: 503⁄524
For Use With (Equipment): Flow Cytometer, Fluorescence Microscope
Format: Slide(s), Tube(s)
Label or Dye: R-PE, SYTOX™ Green
Number of Reactions: 50
Product Line: SYTOX®
Product Size: 1 kit
Shipping Condition: Wet Ice

Contents & storage

Contains 1 vial of annexin V, R-PE conjugate (250 µL), 1 vial of SYTOX® green stain (100 µL), and 1 bottle of annexin binding buffer (5X solution, 15 mL).

Store in refrigerator (2–8°C) and protect from light.


Manuals & protocols