Invitrogen™

Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry

Catalog number: V35113
Invitrogen™

Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry

Catalog number: V35113
Easily differentiate live, dead, and apoptotic cells during flow cytometry with our Dead Cell Apoptosis kits with Annexin V conjugates including Alexa Fluor 488, FITC, propidium iodide, PE, APC, and SYTOX Green. These conjugates distinguish live, dead, or apoptotic cells via different dyes, which is vital for confirming cell viability and apoptosis using multi-parametric studies.
 
Catalog Number
V35113
Unit Size
1 kit
Label or Dye
SYTOX Green, APC (Allophycocyanin)
Excitation
503, 650
Emission
524, 660
Price (USD)
540.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptionDead Cell Apoptosis Kit with Annexin V APC and SYTOX Green, for flow cytometry
Emission524, 660
Excitation503, 650
Flow Cytometer Laser Lines633/635, 488
Label or DyeSYTOX Green, APC (Allophycocyanin)
No. of Reactions50
Shipping ConditionWet Ice
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Kit ContentsContains 1 vial of annexin V, APC conjugate (250 µL), 1 vial of SYTOX green stain (100 µL),and 1 bottle of annexin binding buffer (5X solution, 15 mL) .
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Product TypeApoptosis Detection Kit
Unit Size1 kit
Catalog Number
Unit Size
50 assays
Label or Dye
Alexa Fluor 488, propidium iodide (PI)
Excitation
499, 535
Emission
521, 617
Price (USD)
175.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptionDead Cell Apoptosis Kit with Annexin V Alexa Fluor 488 & Propidium Iodide (PI)
Emission521, 617
Excitation499, 535
Flow Cytometer Laser Lines488
Label or DyeAlexa Fluor 488, propidium iodide (PI)
Shipping ConditionWet Ice
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Kit ContentsContains 1 vial of annexin V, Alexa Fluor 488 conjugate (250 µL), 1 vial of propidium iodide (PI, 100 µL), and 1 bottle of annexin binding buffer (5X solution, 15 mL).
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Product TypeApoptosis Detection Kit
Unit Size50 assays
Catalog Number
Unit Size
1 kit
Label or Dye
FITC (Fluorescein), propidium iodide (PI)
Excitation
494, 535
Emission
518, 617
Price (USD)
267.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptionDead Cell Apoptosis Kit with Annexin V FITC and PI, for flow cytometry
Emission518, 617
Excitation494, 535
Flow Cytometer Laser Lines488
Label or DyeFITC (Fluorescein), propidium iodide (PI)
No. of Reactions50
Shipping ConditionWet Ice
Detection MethodFluorescent
For Use With (Equipment)Flow Cytometer
Kit ContentsContains 1 vial of annexin V, FITC conjugate (250 µL ), 1 vial of propidium iodide (PI, 100 µL), and 1 bottle of annexin binding buffer (5X solution, 15 mL).
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Product TypeApoptosis Detection Kit
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Label or Dye
SYTOX Green, R-PE
Excitation
503, 488
Emission
524, 575
Price (USD)
562.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptionDead Cell Apoptosis Kit with Annexin V PE and SYTOX™ Green, for flow cytometry
Emission524, 575
Excitation503, 488
Flow Cytometer Laser Lines561, 488
Label or DyeSYTOX Green, R-PE
No. of Reactions50
Shipping ConditionWet Ice
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Kit ContentsContains 1 vial of annexin V, R-PE conjugate (250 µL), 1 vial of SYTOX green stain (100 µL), and 1 bottle of annexin binding buffer (5X solution, 15 mL).
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Product TypeApoptosis Detection Kit
Unit Size1 kit
Catalog Number
V13245
also known as V-13245
Unit Size
250 assays
Label or Dye
Alexa Fluor 488, propidium iodide (PI)
Excitation
499, 535
Emission
521, 617
Price (USD)
604.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptionDead Cell Apoptosis Kit with Annexin V Alexa Fluor 488 & Propidium Iodide (PI)
Emission521, 617
Excitation499, 535
Flow Cytometer Laser Lines488
Label or DyeAlexa Fluor 488, propidium iodide (PI)
Shipping ConditionWet Ice
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Kit ContentsContains 5 vials of annexin V, Alexa Fluor 488 conjugate (250 µL per vial), 1 vial of propidium iodide (PI, 100 µL), and 1 bottle of annexin binding buffer (5X solution, 50 mL).
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Product TypeApoptosis Detection Kit
Unit Size250 assays
Showing 5 of 5
Catalog NumberSpecificationsUnit SizeLabel or DyeExcitationEmissionPrice (USD)AvailabilityQuantity
V35113Full specifications
1 kitSYTOX Green, APC (Allophycocyanin)503, 650524, 660
540.00
Your Price:
***
DescriptionDead Cell Apoptosis Kit with Annexin V APC and SYTOX Green, for flow cytometry
Emission524, 660
Excitation503, 650
Flow Cytometer Laser Lines633/635, 488
Label or DyeSYTOX Green, APC (Allophycocyanin)
No. of Reactions50
Shipping ConditionWet Ice
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Kit ContentsContains 1 vial of annexin V, APC conjugate (250 µL), 1 vial of SYTOX green stain (100 µL),and 1 bottle of annexin binding buffer (5X solution, 15 mL) .
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Product TypeApoptosis Detection Kit
Unit Size1 kit
V13241Full specifications
50 assaysAlexa Fluor 488, propidium iodide (PI)499, 535521, 617
175.00
Your Price:
***
DescriptionDead Cell Apoptosis Kit with Annexin V Alexa Fluor 488 & Propidium Iodide (PI)
Emission521, 617
Excitation499, 535
Flow Cytometer Laser Lines488
Label or DyeAlexa Fluor 488, propidium iodide (PI)
Shipping ConditionWet Ice
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Kit ContentsContains 1 vial of annexin V, Alexa Fluor 488 conjugate (250 µL), 1 vial of propidium iodide (PI, 100 µL), and 1 bottle of annexin binding buffer (5X solution, 15 mL).
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Product TypeApoptosis Detection Kit
Unit Size50 assays
V13242Full specifications
1 kitFITC (Fluorescein), propidium iodide (PI)494, 535518, 617
267.00
Your Price:
***
DescriptionDead Cell Apoptosis Kit with Annexin V FITC and PI, for flow cytometry
Emission518, 617
Excitation494, 535
Flow Cytometer Laser Lines488
Label or DyeFITC (Fluorescein), propidium iodide (PI)
No. of Reactions50
Shipping ConditionWet Ice
Detection MethodFluorescent
For Use With (Equipment)Flow Cytometer
Kit ContentsContains 1 vial of annexin V, FITC conjugate (250 µL ), 1 vial of propidium iodide (PI, 100 µL), and 1 bottle of annexin binding buffer (5X solution, 15 mL).
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Product TypeApoptosis Detection Kit
Unit Size1 kit
V35112Full specifications
1 kitSYTOX Green, R-PE503, 488524, 575
562.00
Your Price:
***
DescriptionDead Cell Apoptosis Kit with Annexin V PE and SYTOX™ Green, for flow cytometry
Emission524, 575
Excitation503, 488
Flow Cytometer Laser Lines561, 488
Label or DyeSYTOX Green, R-PE
No. of Reactions50
Shipping ConditionWet Ice
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Kit ContentsContains 1 vial of annexin V, R-PE conjugate (250 µL), 1 vial of SYTOX green stain (100 µL), and 1 bottle of annexin binding buffer (5X solution, 15 mL).
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Product TypeApoptosis Detection Kit
Unit Size1 kit
V13245
also known as V-13245
Full specifications
250 assaysAlexa Fluor 488, propidium iodide (PI)499, 535521, 617
604.00
Your Price:
***
DescriptionDead Cell Apoptosis Kit with Annexin V Alexa Fluor 488 & Propidium Iodide (PI)
Emission521, 617
Excitation499, 535
Flow Cytometer Laser Lines488
Label or DyeAlexa Fluor 488, propidium iodide (PI)
Shipping ConditionWet Ice
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Kit ContentsContains 5 vials of annexin V, Alexa Fluor 488 conjugate (250 µL per vial), 1 vial of propidium iodide (PI, 100 µL), and 1 bottle of annexin binding buffer (5X solution, 50 mL).
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Product TypeApoptosis Detection Kit
Unit Size250 assays
Showing 5 of 5
There are several advantages to using Dead Cell Apoptosis kits with Annexin V for flow cytometry in order to assay cell viability:

Superior brightness
Unlike other Annexin V kits that have lower protein concentrations or purification levels, the Annexin V Alexa Fluor 488 conjugate is optimized for flow cytometry to provide the largest separation between apoptotic and live cells. The Alexa Fluor 488 dye is a superior green-fluorescent dye with a spectrum similar to fluorescein (FITC).

High binding efficiency
Annexin V conjugates are made from a highly purified cys-annexin, resulting in higher binding efficiency and extremely accurate characterization of the apoptotic process.

Multi-parametric
Many publications require a minimum of two different ways to identify apoptotic cells. This multi-parametric kit detects phosphatidyl serine (PS) on the cytoplasmic surface of the cell membrane, while membrane integrity is determined via propidium iodide.

Dead Cell Apoptosis Kit with Annexin V Alexa Fluor 488 & PI
The Dead Cell Apoptosis Kit with Annexin V Alexa Fluor 488 & Propidium Iodide (PI) (V13241, V13245) is used in flow cytometry to measure early apoptosis by detecting phosphatidyl serine (PS) expression and membrane permeability. When cells are stained with Annexin V and propidium iodide, apoptotic cells expressing PS show green fluorescence, which can be detected in the FITC channel, and low red fluorescence. Dead or necrotic cells show bright red fluorescence and no green fluorescence, while live cells show no green or red fluorescence.

Dead Cell Apoptosis Kit with Annexin V PE and SYTOX Green
The Dead Cell Apoptosis Kit with Annexin V PE and SYTOX Green detects PS externalization in apoptotic cells during flow cytometry using recombinant annexin V conjugated to the orange fluorescent phycobiliprotein R-PE, while dead cells are detected with SYTOX Green nucleic acid stain. After treatment with both probes, apoptotic cells show orange fluorescence, dead cells show green fluorescence, and live cells show little or no fluorescence. These populations can easily be distinguished in the 530/30 nm and 585/42 nm bandpass filters with a 488 nm laser flow cytometer.

Dead Cell Apoptosis Kit with Annexin V APC and SYTOX Green
The Dead Cell Apoptosis Kit with Annexin V APC and SYTOX Green detects PS externalization in apoptotic cells during flow cytometry using recombinant annexin V conjugated to red laser-excited allophycocyanin, and dead cells using SYTOX Green nucleic acid stain. Apoptotic cells are detected by annexin V binding to externalized PS. Live cells show little or no fluorescence, apoptotic cells show red fluorescence and very little green fluorescence, and late apoptotic cells show a higher level of red and orange fluorescence. These populations can easily be distinguished using a flow cytometer that has both 488 nm and 633 nm excitation sources (an argon-ion laser and a HeNe laser).

The Dead Cell Apoptosis Kit with Annexin V FITC and PI
The Dead Cell Apoptosis Kit with Annexin V FITC and PI detects PS externalization in apoptotic cells using recombinant annexin V conjugated to green-fluorescent FITC dye and dead cells using PI. Necrotic cells that stain with PI show red fluorescence. After treatment with both probes, apoptotic cells show green fluorescence, dead cells show red and green fluorescence, and live cells show little or no fluorescence.

For Research Use Only. Not for use in diagnostic procedures.

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Frequently asked questions (FAQs)

Citations & References