Invitrogen™

Dead Cell Apoptosis Kit with Annexin V APC and SYTOX™ Green, for flow cytometry

Catalog number:  V35113

Related applications:

Cell Analysis

| Cell Analysis

| Industrial & Applied Science

| Cell Analysis

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Description

This flow cytometry product detects the externalization of phosphatidylserine in apoptotic cells using recombinant annexin V conjugated to red laser-excited allophycocyanin, and dead cells using SYTOX™ Green nucleic acid stain. Apoptotic cells are detected by annexin V binding to externalized phosphatidylserine, and late apoptotic and necrotic cells have compromised membranes that permit SYTOX™ Green stain access to cellular nucleic acids. After exposing a cell population to APC annexin V and SYTOX™ Green stain, live cells show little or no fluorescence, apoptotic cells show red fluorescence and very little green fluorescence, and late apoptotic cells show a higher level of red and orange fluorescence. These populations can easily be distinguished using a flow cytometer that has both 488 nm and 633 nm excitation sources (an argon-ion laser and a HeNe laser).

View a selection guide for all apoptosis assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Excitation/Emission: SYTOX® green: 503⁄524, APC: 650⁄660
Flow Cytometer Laser Lines: 633/635, 488
Label Type: Classic Dyes, Other Label(s) or Dye(s)
Label or Dye: APC (Allophycocyanin), SYTOX™ Green
No. of Reactions: 50
Product Line: SYTOX®
Shipping Condition: Wet Ice
Detection Method: Fluorescent
For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
Format: Tube(s), Slide(s)

Contents & storage

Contains 1 vial of annexin V, APC conjugate (250 µL), 1 vial of SYTOX® green stain (100 µL),and 1 bottle of annexin binding buffer (5X solution, 15 mL) .

Store in refrigerator (2–8°C) and protect from light.