Invitrogen™

Metabolic Activity Dead Cell Apoptosis Kit with C12 Resazurin, Annexin V APC, and SYTOX™ Green, for flow cytometry

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Description

This flow cytometry product provides a three-color fluorescence assay that distinguishes live, apoptotic, and late apoptotic cells from one another. These populations can easily be distinguished using a flow cytometer that has both 488 nm and 633 nm excitation sources (an argon-ion laser and a HeNe laser) and the following reagents Annexin V to detect phosphatidylserine, C12 resazurin for cell metabolism, and SYTOX™ Green nucleic acid stain for compromised membranes.

View a selection guide for all apoptosis assays for flow cytometry.
WARNING: Cancer and Reproductive Harm – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Excitation/Emission: C12-Resazurin: 571⁄585, SYTOX® green: 503⁄524, APC: 650⁄660
Flow Cytometer Laser Lines: 633/635, 488
Label Type: Classic Dyes, Other Label(s) or Dye(s)
Label or Dye: APC (Allophycocyanin), SYTOX™ Green, C12-resazurin
No. of Reactions: 50
Product Line: SYTOX®
Shipping Condition: Wet Ice
Detection Method: Fluorescent
For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
Format: Tube(s), Slide(s)

Contents & storage

Contains 1 vial of annexin V, APC conjugate (250 µL), 1 vial of SYTOX® green stain (100 µL), 1 vial of C12-resazurin (40 µg), and 1 vial of DMSO (1.5 mL).

Store in refrigerator (2–8°C) and protect from light.