Metabolic Activity Dead Cell Apoptosis Kit with C12 Resazurin, Annexin V APC, and SYTOX® Green, for flow cytometry

Catalog number: V35114

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function | Cellular Imaging | Flow Cytometry | Target-Based ADME⁄Tox Assays

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Description

This flow cytometry product provides a three-color fluorescence assay that distinguishes live, apoptotic, and late apoptotic cells from one another. These populations can easily be distinguished using a flow cytometer that has both 488 nm and 633 nm excitation sources (an argon-ion laser and a HeNe laser) and the following reagents Annexin V to detect phosphatidylserine, C12 resazurin for cell metabolism, and SYTOX™ Green nucleic acid stain for compromised membranes.

View a selection guide for all apoptosis assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: Slide(s), Tube(s)
Label or Dye: APC (Allophycocyanin), SYTOX® Green, C12-resazurin
Product Size: 1 kit
Detection Method: Fluorescent
Number of Reactions: 50
For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
Excitation⁄Emission (nm): C12-Resazurin: 571⁄585, APC: 650⁄660, SYTOX® green: 503⁄524
Shipping Condition: Wet Ice

Contents & storage

Contains 1 vial of annexin V, APC conjugate (250 µL), 1 vial of SYTOX® green stain (100 µL), 1 vial of C12-resazurin (40 µg), and 1 vial of DMSO (1.5 mL).

Store in refrigerator (2–8°C) and protect from light.

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