pTrcHis2 A, B, & C Bacterial Expression Vectors

Catalog number: V36520

Invitrogen™  Related applications: Protein Expression

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Description

Our pTrcHis A, B, & C vectors are designed to offer enhanced translation initiation and high-level expression in E. coli. These vectors feature:

• High-level regulated transcription from the trc promoter
• Enhanced translation efficiency of eukaryotic genes in E. coli
• The lacO operator and lacIq repressor gene for transcriptional regulation in any E. coli strain

This particular vector offers:

• C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an anti-His(C-term) antibody
• C-terminal c-myc epitope for easy detection of fusion proteins with an anti-myc antibody

For N-terminal polyhistidine tag and Xpress™ epitope, please see our pTrcHis A, B, & C Vector.
For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Vector Type: pTrc
Cloning Method: Restriction Enzyme ⁄ MCS
Promoter: Trc and lacO
Protein Tag or Fusion: Thioredoxin
Antibiotic Resistance (Bacterial): Ampicillin (AmpR)
Cleavage: EK (Enterokinase) Recognition Site
Constitutive or Inducible System: Inducible
Inducing Agent: IPTG
Product Size: 20 µg
Selection Agent (Eukaryotic): None

Contents & storage

All vectors are supercoiled and are provided lyophilized. A TOP10 E. coli stab and positive expression control are provided. Store vectors at -20°C. Store stab at room temperature. The vector is guaranteed stable for 6 months when properly stored.

Documents

Manuals & protocols