Our pTrcHis A, B, & C vectors are designed to offer enhanced translation initiation and high-level expression in E. coli. These vectors feature:
• High-level regulated transcription from the trc promoter • Enhanced translation efficiency of eukaryotic genes in E. coli • The lacO operator and lacIq repressor gene for transcriptional regulation in any E. coli strain
This particular vector offers:
• C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an anti-His(C-term) antibody • C-terminal c-myc epitope for easy detection of fusion proteins with an anti-myc antibody
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Constitutive or Inducible System
Inducible
Inducing Agent
IPTG
Promoter
Trc, lacO
Product Type
Bacterial Expression Vector
Selection Agent (Eukaryotic)
None
Antibiotic Resistance Bacterial
Ampicillin (AmpR)
Cleavage
EK (Enterokinase) Recognition Site
Protein Tag
Thioredoxin
Cloning Method
Restriction Enzyme/MCS
Vector
pTrc
Contents & Storage
All vectors are supercoiled and are provided lyophilized. A TOP10 E. coli stab and positive expression control are provided. Store vectors at -20°C. Store stab at room temperature. The vector is guaranteed stable for 6 months when properly stored.