pLenti6.2/V5-DEST™ Gateway® Vector

Catalog number: V36820

Invitrogen™  Related applications: Gateway Cloning | Protein Expression

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Description

The pLenti6.2⁄V5-DEST™ Gateway® Vector is a Gateway®-adapted ViraPower™ II lentiviral expression vector for lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving constitutive expression of the target gene and the PGK promoter for driving long-term, persistent expression of the Blasticidin stable selection marker.

Advantages
• Stable expression
• Long-term experiments
• High-throughput screening
• Accurate titer of functional virus (using Blasticidin method)
• Flexible and versatile Gateway® recombination cloning technology

Key Features
• Human cytomegalovirus (CMV) immediate early promoter to control high-level expression of the gene of interest
• PKG Promoter for expression of Blasticidin selection marker

Kit includes
• pLenti6.2⁄V5-DEST™ Gateway® Vector box
• One Shot® Stbl3™ chemically competent E. coli (C7373-03)

Related SKUs
• 293FT Cell Line (R70007; R70007)
• ViraPower™ Lentiviral Support Kit (K4970-00)
• ViraPower™ Lentiviral Packaging Mix (K4975-00)
• Lipofectamine® 2000 (11668-019; 11668-027)

For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Promoter: CMV
Key Function: Viral Expression
Protein Tag or Fusion: V5 Epitope Tag
Delivery Method: Lentiviral
Cloning Method: Gateway®
Selection Agent (Eukaryotic): Blasticidin
Vector Type: pLenti
Product Size: 6 µg
Selection Marker Promoter: PGK Promoter
Constitutive or Inducible System: Constitutive

Contents & storage

pLenti6.2⁄V5-DEST™ Gateway® Vector Box contains pLenti6.2⁄V5-DEST, 40 µl of 150 ng⁄µl vector in 10 mM Tris-HCl, 1mM EDTA, pH 8.0 and pLenti6.2⁄V5-GW⁄lacZ control, 20 µl of 0.5 µg⁄µl vector in 10 mM Tris-HCl, 1mM EDTA, pH 8.0. Store at –20°C.

One Shot® Stbl3™ chemically competent E. coli box contains transformation reagents including 20 single-use 50 µl aliquots of One Shot® Competent Cells, S.O.C. medium, and pUC19 supercoiled plasmid control. Store at -80°C.

Documents

Manuals & protocols

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