pLenti6.2-GW/EmGFP Expression Control Vector

Catalog number: V36920

Invitrogen™  Related applications: Cellular Imaging | Gateway Cloning | Protein Assays and Analysis | Protein Expression

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Description

The Vivid Colors™ pLenti6.2-GW⁄EmGFP Expression Control Vector is a ViraPower™ positive control lentiviral vector containing Emerald Green Fluorescent Protein (EmGFP). It is designed for use with the ViraPower™ Lentiviral Expression Systems as a positive control to enable the detection of EmGFP fluorescence following transfection in 293FT cells, as a titer control to produce an EmGFP-expressing lentivirus stock and as a transduction control following transduction in both dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving constitutive expression of EmGFP and the PGK promoter for driving long-term, persistent expression of the Blasticidin stable selection marker. This is not a cloning vector.

Advantages
• Optimization of viral transduction efficiency
• Optimization of 293FT Transfection
• 2 day titer of functional virus using EmGFP

Key Features
• Human cytomegalovirus (CMV) immediate early promoter to control high-level expression of the EmGFP
• PKG Promoter for expression of Blasticidin selection marker

Kit Includes
pLenti6.2-GW⁄EmGFP Vector

Related SKUs
• 293FT Cell Line (R70007; R70007)
• ViraPower™ Lentiviral Support Kit (K4970-00)
• ViraPower™ Lentiviral Packaging Mix (K4975-00)
• Lipofectamine® 2000 (11668-019; 11668-027)

For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: CMV
Key Function: Viral Expression, Reporter Assays
Protein Tag or Fusion: Untagged
Reporter: GFP (EmGFP)
Delivery Method: Lentiviral
Cloning Method: Gateway®
Selection Agent (Eukaryotic): Blasticidin
Vector Type: pLenti
Product Size: 1 each
Selection Marker Promoter: PGK Promoter
Constitutive or Inducible System: Constitutive

Contents & storage

pLenti6.2-GW⁄EmGFP Vector: 20 µg of control vector is supplied in solution as 40 µl of 0.5 µg⁄µl in 10 mM Tris–HCl, 1 mM EDTA, pH 8.0. Store at –20°C.

Documents

Manuals & protocols