Vivid Colors™ pLenti6.3/V5-GW/EmGFP Expression Control Vector

Catalog number: V37006

Invitrogen™  Related applications: Cellular Imaging | Protein Assays and Analysis | Protein Expression

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Description

The Vivid Colors™ pLenti6.3⁄V5-GW⁄EmGFP Expression Control Vector is a ViraPower™ HiPerform™ positive control lentiviral vector containing Emerald Green Fluorescent Protein (EmGFP). It is designed for use with the ViraPower™ HiPerform™ Lentiviral Expression Systems as a positive control to enable the detection of higher levels of EmGFP fluorescence following transfection in 293FT cells, as a titer control to produce an EmGFP-expressing lentivirus stock and as a transduction control following transduction in both dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving constitutive expression of EmGFP and the SV40 promoter for driving expression of the blasticidin stable selection marker. The vector is equipped with two key genetic elements, making them Hiperform™ vectors: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in EmGFP expression compared to vectors lacking these elements. This control vector is not designed for generating EmGFP fusion proteins and does not express the V5 epitope.

Advantages
• Lentivirus based expression of EmGFP in dividing and non-dividing mammalian cells
• Serve as a quick positive control for transfection and lentiviral production
• Serve as a quick titer control in determination of lentivirus titer

Key Features
• Constitutive expression with CMV promoter
• High level expression of EmGFP without V5 epitope
• WPRE and cPPT sequences produce at least 4-fold increase in EmGFP expression compared to other Lenti vectors without these elements
• Blasticidin selection marker for stable selection

Kit Includes
• pLenti6.3⁄V5-GW⁄EmGFP Expression Control Vector

Related SKUs
• ViraPower™ HiPerform™ Lentiviral TOPO® Expression Kit (K531000)
• ViraPower™ HiPerform™ Lentiviral Gateway® Expression Kit (K533000)
• ViraPower™ HiPerform™ T-Rex™ Gateway® Expression System (A11141)
• ViraPower™ HiPerform™ Promoterless Gateway® Expression System (A11145)

For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: CMV
Key Function: Reporter Assays, Viral Expression
Protein Tag or Fusion: V5 Epitope Tag
Reporter: GFP (EmGFP)
Delivery Method: Lentiviral
Cloning Method: Gateway®
Selection Agent (Eukaryotic): Blasticidin
Vector Type: pLenti
Constitutive or Inducible System: Constitutive
Product Size: 20 µg

Contents & storage

pLenti6.3⁄V5-GW⁄EmGFP vector: 20 µg (40 µl of 0.5 µg⁄µl vector in TE buffer, pH 8.0), store at —20°C

Documents

Manuals & protocols