'The recruitment of FtsZ to the septum and its subsequent interaction with other cell division proteins in a spatially and temporally controlled manner are the keys to bacterial cell division. In the present study, we have tested the hypothesis that FtsZ and FtsW of Mycobacterium tuberculosis could be binding partners. ... More
Tight Binding Inhibition of Protein Phosphatase-1 by Phosphatidic Acid. SPECIFICITY OF INHIBITION BY THE PHOSPHOLIPID.
Authors Jones Jeffrey A; Hannun Yusuf A;
JournalJ Biol Chem
PubMed ID11856740
'Phosphatidic acid (PA) has been identified as a bioactive lipid second messenger, yet despite extensive investigation, no cellular target has emerged as a mediator of its described biological effects. In this study, we identify the gamma isoform of the human protein phosphatase-1 catalytic subunit (PP1cgamma) as a high affinity in ... More
Biochemical properties of the PsbS subunit of photosystem II either purified from chloroplast or recombinant.
'The biochemical properties of PsbS protein, a nuclear-encoded Photosystem II subunit involved in the high energy quenching of chlorophyll fluorescence, have been studied using preparations purified from chloroplasts or obtained by overexpression in bacteria. Despite the homology with chlorophyll a/b/xanthophyll-binding proteins of the Lhc family, native PsbS protein does not ... More
Genetic and biochemical properties of streptococcal NAD-glycohydrolase inhibitor.
AuthorsKimoto H, Fujii Y, Hirano S, Yokota Y, Taketo A,
JournalJ Biol Chem
PubMed ID16380378
'The gene encoding streptolysin O (slo), a cytolysin of hemolytic streptococci, is transcribed polycistronically from the promoter of the preceding NAD-glycohydrolase (NADase) gene (nga). Between nga and slo, a putative open reading frame (orf1) is located whose function has been totally unknown. Present investigation demonstrated that the orf1 encodes a ... More
Assimilation of nicotinamide mononucleotide requires periplasmic AphA phosphatase in Salmonella enterica.
AuthorsGrose JH, Bergthorsson U, Xu Y, Sterneckert J, Khodaverdian B, Roth JR,
JournalJ Bacteriol
PubMed ID15968063
'Salmonella enterica can obtain pyridine from exogenous nicotinamide mononucleotide (NMN) by three routes. In route 1, nicotinamide is removed from NMN in the periplasm and enters the cell as the free base. In route 2, described here, phosphate is removed from NMN in the periplasm by acid phosphatase (AphA), and ... More
Interaction of the Conserved Region 4.2 of sigma E with the RseA Anti-sigma Factor.
Authors Tam Christina; Collinet Bruno; Lau Gary; Raina Satish; Missiakas Dominique;
JournalJ Biol Chem
PubMed ID12016219
Evarsigma(E) RNA polymerase transcribes a regulon of folding factors for the bacterial envelope and is induced by physical and chemical stresses. The RseA anti-sigma factor inhibits the activity of Evarsigma(E) RNA polymerase. It is shown here that the N-terminal portion of varsigma(E), residues 1-153, binds core RNA polymerase. RseA interacts ... More
Biochemical diversity among the 1-amino-cyclopropane-1-carboxylate synthase isozymes encoded by the Arabidopsis gene family.
AuthorsYamagami T, Tsuchisaka A, Yamada K, Haddon WF, Harden LA, Theologis A,
JournalJ Biol Chem
PubMed ID12968022
1-Amino-cyclopropane-1-carboxylate synthase (ACS, EC 4.4.1.14) is the key enzyme in the ethylene biosynthetic pathway in plants. The completion of the Arabidopsis genome sequence revealed the presence of twelve putative ACS genes, ACS1-12, dispersed among five chromosomes. ACS1-5 have been previously characterized. However, ACS1 is enzymatically inactive whereas ACS3 is a ... More
FarR Regulates the farAB-Encoded Efflux Pump of Neisseria gonorrhoeae via an MtrR Regulatory Mechanism.
The farAB operon of Neisseria gonorrhoeae encodes an efflux pump which mediates gonococcal resistance to antibacterial fatty acids. It was previously observed that expression of the farAB operon was positively regulated by MtrR, which is a repressor of the mtrCDE-encoded efflux pump system (E.-H. Lee and W. M. Shafer, Mol. ... More
A quorum sensing-associated virulence gene of Pseudomonas aeruginosa encodes a LysR-like transcription regulator with a unique self-regulatory mechanism.
Authors Cao H; Krishnan G; Goumnerov B; Tsongalis J; Tompkins R; Rahme L G;
JournalProc Natl Acad Sci U S A
PubMed ID11724939
The human opportunistic pathogen Pseudomonas aeruginosa strain PA14 infects both plants and animals. Previously, using plants to screen directly for P. aeruginosa virulence-attenuated mutants, we identified a locus, pho34B12, relevant in mammalian pathogenesis. Here, nonsense point mutations in the two opposing ORFs identified in the pho34B12 locus revealed that one ... More
Tetrahymena proteins p80 and p95 are not core telomerase components.
Authors Mason D X; Autexier C; Greider C W;
JournalProc Natl Acad Sci U S A
PubMed ID11592988
Telomeres provide stability to eukaryotic chromosomes and consist of tandem DNA repeat sequences. Telomeric repeats are synthesized and maintained by a specialized reverse transcriptase, termed telomerase. Tetrahymena thermophila telomerase contains two essential components: Tetrahymena telomerase reverse transcriptase (tTERT), the catalytic protein component, and telomerase RNA that provides the template for ... More