pAd/CMV/V5-DEST™ Gateway™ Vector Kit

Citas y referencias (4)

Invitrogen™

pAd/CMV/V5-DEST™ Gateway™ Vector Kit

El kit de vectores pAd⁄CMV⁄V5-DEST™ Gateway™ contiene el vector de expresión adenoviral ViraPower™ adaptado para Gateway™. El vector pAd⁄CMV⁄V5-DEST™ esMás información

Número de catálogo	Cantidad
V49320 también denominado V493-20	6 μg

Número de catálogo V49320

también denominado V493-20

Precio (MXN)

Cantidad:

El kit de vectores pAd⁄CMV⁄V5-DEST™ Gateway™ contiene el vector de expresión adenoviral ViraPower™ adaptado para Gateway™. El vector pAd⁄CMV⁄V5-DEST™ es ideal para una clonación de recombinación sencilla y para la expresión transitoria basada en adenovirus de un gen diana en células de mamíferos que se dividen y no se dividen. El vector permite la generación de un adenovirus que contiene el gen diana donde promotor de CMV impulsa la expresión constitutiva de alto nivel.

Ventajas
• Clonación de recombinación altamente eficaz y rápida
• Produce soluciones madre de gran carga adenovirales
• Administración eficaz del gen «in vitro» o «in vivo» en células de mamíferos que se dividen y no se dividen células
• Produce un virus incompetente para la replicación para mejorar la bioseguridad del sistema
• Se puede utilizar en aplicaciones de alto rendimiento

Características clave
• Tecnología Gateway™ para una clonación rápida y eficaz
• Promotor de CMV para la expresión constitutiva de alto rendimiento del gen de interés
• Secuencias de Ad5 humano (ΔE3) y repeticiones terminales invertidas del virus (ITR) para el envasado de la construcción de la expresión en viriones
• Secuencia de poliadenilación de la timidina cinasa (TK) del virus del herpes simple para una terminación de la transcripción eficaz y para la poliadenilación del ARNm
• Epítopo V5 para la detección de proteína recombinante
• Marcador de selección de ampicilina

Contenido del kit
• Vector pAd⁄CMV⁄V5-DEST™ Gateway™
•Plásmido de control pAd⁄CMV⁄V5-GW⁄lacZ

Para uso exclusivo en investigación. No diseñado para uso diagnóstico o terapéutico.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Sistema constitutivo o inducibleConstitutivo

Tipo de entregaAdenoviral

Para utilizar con (aplicación)Expresión viral

Tipo de productoVector de expresión de mamíferos

Cantidad6 μg

VectorpDEST

Método de clonaciónGateway

Línea de productosGateway, ViraPower

PromotorCMV

Etiqueta de proteínaEtiqueta de epítopo V5

Unit Size6 µg

Contenido y almacenamiento

• 150 ng⁄μl de vector pAd-CMV⁄V5-DEST™ en tampón de TE, pH 8.0. 40μl (conservar a -20°C)
• pAd⁄CMV⁄V5-GW⁄ plásmido de control lacZ 1μg⁄μl en tampón de TE, pH 8.0. 10μl (conservar a -20 °C)

Preguntas frecuentes

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all pAd/CMV/V5-DEST™ Gateway™ Vector Kit FAQs

Citations & References (4)

Citations & References

Abstract

Disruption of glucose sensing and insulin secretion by ribozyme Kir6.2-gene targeting in insulin-secreting cells.

Authors:Li L, Rojas A, Wu J, Jiang C,

Journal:Endocrinology

PubMed ID:15166124

'The ATP-sensitive K+ (KATP) channel, composed of Kir6.2 and sulfonylurea receptor (SUR1), in pancreatic beta-cells is believed to serve as a metabolic sensor regulating insulin secretion according to glucose levels. Thus, genetic disruption of Kir6.2 expression may impair KATP channel function in glucose sensing and insulin secretion. Here we show ... More

The receptor for parathyroid hormone and parathyroid hormone-related peptide is hydrolyzed and its signaling properties are altered by directly binding the calpain small subunit.

Authors:Shimada M, Mahon MJ, Greer PA, Segre GV,

Journal:Endocrinology

PubMed ID:15691895

'We show calcium-dependent, direct binding between the N-terminal portion of the PTH/PTHrP receptor (PTH1R) C-terminal intracellular tail and the calpain small subunit. Binding requires, but may not be limited to, amino acids W474, S475, and W477. The wild-type, full-length rat (r) PTH1R, but not rPTH1R with W474A/W477A substitutions, copurifies with ... More

Estrogen related receptors stimulate pyruvate dehydrogenase kinase isoform 4 (PDK4) gene expression.

Authors:Zhang Y, Ma K, Sadana P, Chowdhury F, Gaillard S, Wang F, McDonnell DP, Unterman TG, Elam MB, Park EA,

Journal:J Biol Chem

PubMed ID:17079227

The pyruvate dehydrogenase complex (PDC) catalyzes the conversion of pyruvate to acetyl-CoA in mitochondria and is a key regulatory enzyme in the oxidation of glucose to acetyl-CoA. Phosphorylation of PDC by the pyruvate dehydrogenase kinases (PDK2 and PDK4) inhibits PDC activity. Expression of the PDK genes is elevated in diabetes ... More

Adipose-specific expression, phosphorylation of Ser794 in insulin receptor substrate-1, and activation in diabetic animals of salt-inducible kinase-2.

Authors:Horike N, Takemori H, Katoh Y, Doi J, Min L, Asano T, Sun XJ, Yamamoto H, Kasayama S, Muraoka M, Nonaka Y, Okamoto M,

Journal:J Biol Chem

PubMed ID:12624099

Salt-inducible kinase (SIK), first cloned from the adrenal glands of rats fed a high salt diet, is a serine/threonine protein kinase belonging to an AMP-activated protein kinase family. Induced in Y1 cells at an early stage of ACTH stimulation, it regulated the initial steps of steroidogenesis. Here we report the ... More