Zitierungen und Referenzen (4)

Invitrogen™

pAd/CMV/V5-DEST™ Gateway™ Vector Kit

Katalognummer	Menge
V49320 auch als V493-20 bezeichnet	6μ g

Katalognummer V49320

auch als V493-20 bezeichnet

Preis (EUR)

2.580,00

Menge:

Preis (EUR)

2.580,00

Das pAd⁄CMV⁄V5-DEST™ Gateway™ Vektor-Kit enthält den Gateway™ adaptierten ViraPower™ Adenoviralen Expressionsvektor pAd⁄CMV⁄V5-DEST™ Vektor für einfaches rekombinationsbasiertes Klonen und Adenoviral-basierte Expression eines Zielgens in sich teilenden und nicht teilenden Säugetierzellen. Der Vektor ermöglicht die Erzeugung eines Adenovirus, das das Zielgen enthält, bei dem die konstitutive, hochgradige Expression vom CMV-Promotor angetrieben wird.

Vorteile
• Hohe Effizienz und schnelles Rekombinationsklonieren
• Produziert hochtitrige Adenovirenstämme
• Effiziente Abgabe des Gens an sich teilende und nicht teilende Säugetierzellen in vitro oder in vivo
• Erzeugt ein replikationsinkompetentes Virus für eine verbesserte Biosicherheit des Systems
• Geeignet für den Einsatz in Anwendungen mit hohem Durchsatz

Wichtige Merkmale
• Gateway™ Technologie für effizientes und schnelles Klonen
•CMV-Promotor für hochgradig konstitutive Expression des zu untersuchenden Gens
•Humane Ad5-Sequenzen (E3) und virale invertierte terminale Wiederholungseinheiten (Inverted Terminal Repeats, ITRs) zur Verpackung des Expressionskonstruktes in Virionen
• Herpes Simplex-Virus-Thymidinkinase (TK)-Polyadenylierungssequenz für effizienten Transkriptionsabbruch und Polyadenylierung des mRNA
• V5-Epitop zum Nachweis des rekombinanten Proteins
• Ampicilin-Auswahlmarker

Das Kit enthält
• pAd⁄CMV⁄5-DEST™ Gateway™ Vektor
•pAd⁄CMV⁄V5-GW⁄lacZ-Kontrollplasmid

Nur für Forschungszwecke. Nicht für therapeutische oder diagnostische Zwecke vorgesehen.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

Konstitutives oder induktives SystemKonstitutiv

LiefertypAdenoviral

Zur Verwendung mit (Anwendung)Virale Expression

ProdukttypSäugetier-Expressionsvektor

Menge6μ g

VektorpDEST

KlonierungsmethodeGateway™

ProduktlinieGateway, ViraPower

PromoterCMV

ProteinmarkierungV5-Epitop-Tag

Unit Size6 µg

Inhalt und Lagerung

•pAd-CMV⁄V5-DEST™ Vektor 150 ng⁄µl in TE-Puffer, pH 8,0. 40 µl (Lagerung bei -20 °C)
• pAd⁄CMV⁄V5-GW⁄lacZ-Kontrollplasmid 1 µg⁄µl in TE-Buffer, pH 8,0. 10 µl (Lagerung bei -20 °C)

Häufig gestellte Fragen (FAQ)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all pAd/CMV/V5-DEST™ Gateway™ Vector Kit FAQs

Zitierungen und Referenzen (4)

Zitierungen und Referenzen

Abstract

Disruption of glucose sensing and insulin secretion by ribozyme Kir6.2-gene targeting in insulin-secreting cells.

Authors:Li L, Rojas A, Wu J, Jiang C,

Journal:Endocrinology

PubMed ID:15166124

'The ATP-sensitive K+ (KATP) channel, composed of Kir6.2 and sulfonylurea receptor (SUR1), in pancreatic beta-cells is believed to serve as a metabolic sensor regulating insulin secretion according to glucose levels. Thus, genetic disruption of Kir6.2 expression may impair KATP channel function in glucose sensing and insulin secretion. Here we show ... More

The receptor for parathyroid hormone and parathyroid hormone-related peptide is hydrolyzed and its signaling properties are altered by directly binding the calpain small subunit.

Authors:Shimada M, Mahon MJ, Greer PA, Segre GV,

Journal:Endocrinology

PubMed ID:15691895

'We show calcium-dependent, direct binding between the N-terminal portion of the PTH/PTHrP receptor (PTH1R) C-terminal intracellular tail and the calpain small subunit. Binding requires, but may not be limited to, amino acids W474, S475, and W477. The wild-type, full-length rat (r) PTH1R, but not rPTH1R with W474A/W477A substitutions, copurifies with ... More

Estrogen related receptors stimulate pyruvate dehydrogenase kinase isoform 4 (PDK4) gene expression.

Authors:Zhang Y, Ma K, Sadana P, Chowdhury F, Gaillard S, Wang F, McDonnell DP, Unterman TG, Elam MB, Park EA,

Journal:J Biol Chem

PubMed ID:17079227

The pyruvate dehydrogenase complex (PDC) catalyzes the conversion of pyruvate to acetyl-CoA in mitochondria and is a key regulatory enzyme in the oxidation of glucose to acetyl-CoA. Phosphorylation of PDC by the pyruvate dehydrogenase kinases (PDK2 and PDK4) inhibits PDC activity. Expression of the PDK genes is elevated in diabetes ... More

Adipose-specific expression, phosphorylation of Ser794 in insulin receptor substrate-1, and activation in diabetic animals of salt-inducible kinase-2.

Authors:Horike N, Takemori H, Katoh Y, Doi J, Min L, Asano T, Sun XJ, Yamamoto H, Kasayama S, Muraoka M, Nonaka Y, Okamoto M,

Journal:J Biol Chem

PubMed ID:12624099

Salt-inducible kinase (SIK), first cloned from the adrenal glands of rats fed a high salt diet, is a serine/threonine protein kinase belonging to an AMP-activated protein kinase family. Induced in Y1 cells at an early stage of ACTH stimulation, it regulated the initial steps of steroidogenesis. Here we report the ... More