pAd/CMV/V5-DEST™ Gateway® Vector Kit

Catalog number: V49320

Invitrogen™  Related applications: Antibody & Cell Culture Production | Gateway Cloning | Protein Expression | Viral Vaccine Production

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The pAd⁄CMV⁄V5-DEST™ Gateway® Vector Kit contains the Gateway®-adapted ViraPower™ adenoviral expression vector, pAd⁄CMV⁄V5-DEST™ vector for easy recombination-based cloning and adenoviral-based, transient expression of a target gene in dividing and non-dividing mammalian cells. The vector allows generation of an adenovirus containing the target gene where constitutive, high-level expression is driven by the CMV promoter.

• High efficiency and rapid recombination cloning
• Produces high titer adenoviral stocks
• Efficient delivery of the gene to dividing and non-dividing mammalian cells in vitro or in vivo
• Produces replication-incompetent virus for enhanced biosafety of the system
• Amenable for use in high-throughput applications

Key Features
• Gateway® Technology for efficient and rapid cloning
• CMV promoter for high-level constitutive expression of gene of interest
• Human Ad5 sequences (ΔE3) and Viral Inverted Terminal Repeats (ITRs) for packaging of the expression construct into virions
• Herpes Simplex Virus thymidine kinase (TK) polyadenylation sequence for efficient transcription termination and polyadenylation of mRNA
• V5 epitope for detection of recombinant protein
• Ampicillin selection marker

Kit Includes
• pAd⁄CMV⁄V5-DEST™ Gateway® Vector
• pAd⁄CMV⁄V5-GW⁄lacZ control plasmid

For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.


Promoter: CMV
Key Function: Viral Expression
Protein Tag or Fusion: V5 Epitope Tag
Delivery Method: Adenoviral
Cloning Method: Gateway®
Vector Type: pDEST
Product Size: 6 µg
Constitutive or Inducible System: Constitutive

Contents & storage

• pAd-CMV⁄V5-DEST™ Vector 150 ng⁄µl in TEBuffer, pH 8.0. 40 µl (Store at -20°C)
• pAd⁄CMV⁄V5-GW⁄lacZ control plasmid 1 µg⁄µl in TE Buffer, pH 8.0. 10 µl (Store at -20°C)


Manuals & protocols