BLOCK-iT™ Pol II miR-luc Validated miRNA Control Vector

Catalog number: V4935100

Invitrogen™  Related applications: Gateway Cloning | RNAi

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Description

The BLOCK-iT™ Pol II miR-luc Validated miRNA Control Vector is specifically designed to allow expression of luc pre-miRNA sequence and contains specific miR flanking sequences that allow proper processing of the miRNA. The engineered luc miRNA produced by the BLOCK-iT™ Pol II miR-luc miR Validated miRNA Vector fully complements it's target site and cleaves the luc mRNA reproduced by reporter plasmids. This control vector is ideal for use with your mammalian cells to familiarize yourself with the vector based miRNA expression system and it can also be used with any BLOCK-iT™ Pol II miR Validated miRNA Vector DuoPak or your own miRNA vector (constructed using the BLOCK-iT™ Pol II miR RNAi Expression Vector Kit) as a positive control for easy analysis of knockdown in any mammalian cell line. This control vector is designed for in vitro and in vivo RNAi analysis.

BLOCK-iT™ Pol II miR-luc Validated miRNA Control Vector for miRNA expression and RNAi analysis in mammalian cells provides the following advantages:
• Rapid and efficient way to perform knockdown experiments with guaranteed results
• Each pre-miRNA sequence is validated to knock down the target gene by at least 70%
• Permits visual or automated selection of cells expressing the pre-miRNA through co-cistronic expression of EmGFP (Emerald GFP)
• Allows transient or stable expression of miRNA in mammalian cells
• Gateway®-adapted vectors for easy recombination of the pre-miRNA sequence from the expression vector to the destination vector of choice for flexible miRNA expression options, including viral expression and expression using tissue-specific or regulated promoters
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: CMV
Reporter: GFP (EmGFP)
RNAi Type: miRNA
Vector Type: BLOCK-iT RNAi Vectors
Product Size: 10 µg
Cloning Method: Gateway®
Delivery Method: Transfection
Selection Agent (Eukaryotic): Blasticidin
Constitutive or Inducible System: Constitutive

Contents & storage

The BLOCK-iT™ Pol II miR-luc Validated miRNA Control Vectors include a pcDNA™6.2-GW/EmGFP-miR plasmid (10 µg) with a pre-miRNA insert designed to the luc gene and one pcDNA™6.2-GW/EmGFP-miR-neg Control Plasmid (10 µg). Store the plasmids at -20°C.

Documents

Manuals & protocols