Invitrogen™

pLenti4/V5-DEST™ Gateway™ Vector

The pLenti4⁄V5-DEST™ Gateway™ Vector is a Gateway™-adapted ViraPower™ lentiviral expression vector for lentiviral-based expression of a target gene in dividingRead more

Catalog Number	Quantity
V49810	6 μg

Catalog number V49810

Price (MXN)

Quantity:

The pLenti4⁄V5-DEST™ Gateway™ Vector is a Gateway™-adapted ViraPower™ lentiviral expression vector for lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving constitutive expression of the target gene and the Zeocin™ selection marker for stable selection in mammalian cells.

Advantages
• Lentivirus based expression of a target gene in dividing and non-dividing mammalian cells

Key Features
• Flexible and versatile Gateway™ recombination cloning technology
• Constitutive high expression with CMV promoter
• Zeocin™ selection marker for stable selection
• C terminal V5 tag for quick detection

Kit includes
• pLenti4⁄V5-DEST™ Gateway™ Vector
• One Shot™ Stbl3™ Chemically Competent E. coli (C7373-03)

Related SKUs
• pLenti6⁄UbC⁄V5-DEST™ Gateway™ Vector (V49910)
• pLenti6⁄V5-DEST™ Gateway™ Vector (V49610)
• pLenti6⁄V5 Directional TOPO™ Cloning Kit (K4955-10)
• ViraPower™ Lentiviral Directional TOPO™ Expression Kit (K495000)
• ViraPower™ Lentiviral Gateway™ Expression Kit (K4960-00)
• ViraPower™ HiPerform™ Lentiviral Gateway™ Expression Kit (K5330-00)

For research use only. Not intended for any therapeutic or diagnostic use.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Constitutive or Inducible SystemConstitutive

Delivery TypeLentiviral

For Use With (Application)Viral Expression

Product TypeLentiviral Expression Vector

Quantity6 μg

Selection Agent (Eukaryotic)Zeocin™

VectorpDEST, pLenti

Cloning MethodGateway

Product LineGateway, ViraPower

PromoterCMV

Protein TagV5 Epitope Tag

Unit Size6 µg

Contents & Storage

ViraPower™ Lentiviral Gateway™ Vectors store at–20°C includes:

pLenti4⁄V5-DEST™: 6 μg (40 μl of 150 ng⁄μl vector in 10 mM Tris-HCl, 1mM EDTA, pH 8.0)
pLenti4⁄V5-GW⁄lacZ control: 10 μg (20 μl of 0.5 μg⁄μl vector in 10 mM Tris-HCl, 1mM EDTA, pH 8.0)

One Shot™ Stbl3™ Chemically Competent E. coli store at –80°C

Frequently asked questions (FAQs)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all pLenti4/V5-DEST™ Gateway™ Vector FAQs