BsdCassette™ Vector (pCMV/Bsd)

Catalog number:  V51020

Invitrogen™  Related applications: Protein Expression

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Blasticidin is a potent selection agent that allows selection of stable mammalian cell lines in as little as one week. The BsdCassette™ vectors simplify the construction of custom vectors that use Blasticidin resistance as a selectable marker. Each BsdCassette™ vector is designed for use in a specific host system and offers:

• A strong host promoter to drive expression of the Blasticidin resistance gene (bsd)(Table 1)
• Two large polylinkers flanking the promoter and bsd gene to facilitate the construction of Blasticidin resistant vectors

Each BsdCassette™ vector carries the synthetic EM-7 promoter for expression of the bsd gene in E. coli. This eliminates the need to add a second antibiotic resistance gene for selection in E. coli. BsdCassette™ vectors provide a eukaryotic promoter for expressionin yeast (pTEF1/Bsd) or mammalian cells (pCMV/Bsd, pEF1/Bsd, pUB/Bsd) for construction of expression vectors with Blasticidin selection for a wide range of host cell types.
For Research Use Only. Not for use in diagnostic procedures.



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Promoter: CMV
Key Function: Constitutive Expression
Protein Tag or Fusion: Untagged
Delivery Method: Transfection
Cloning Method: Restriction Enzyme ⁄ MCS
Selection Agent (Eukaryotic): Blasticidin
Vector Type: pCMV
Constitutive or Inducible System: Constitutive
Product Size: 20 µg

Contents & storage

20 µg of a BsdCassette™ vector is provided supercoiled and lyophilized. The BsdCassette™ Starter Kits include 20 µg of BsdCassette™ vector and 50 mg of Blasticidin. Store the vector and Blasticidin at -20°C. All reagents are guaranteed stable for 6 months when properly stored.


Manuals & protocols