pcDNA™5/FRT/TO Vector Kit

Catalog number: V652020

Invitrogen™  Related applications: Protein Expression

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Description

pcDNA™FRT⁄TO vector is a 5.1 kb inducible expression vector designed for use with the Flp-In™ T-REx™ System. The control plasmid, pcDNA™5⁄FRT⁄TO⁄CAT, is also included for use as a positive control for transfection and expression in your Flp-In™ T-REx™ host cell line of choice.

The pcDNA™5⁄FRT⁄TO vector contains the following elements:

• A hybrid human cytomegalovirus (CMV)⁄TetO2 promoter for high-level, tetracycline-regulated expression of the gene of interest in a wide range of mammalian cells
• Multiple cloning site with 10 unique restriction sites to facilitate cloning the gene of interest
• FLP Recombination Target (FRT) site for Flp recombinase-mediated integration of the vector into the Flp-In™ T-REx™ host cell line
• Hygromycin resistance gene for selection of stable cell lines
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: CMV⁄TO
Key Function: Regulated Expression
Protein Tag or Fusion: Untagged
Delivery Method: Transfection
Cloning Method: Restriction Enzyme ⁄ MCS
Selection Agent (Eukaryotic): Hygromycin
Vector Type: pcDNA
Constitutive or Inducible System: Inducible
Inducing Agent: Tetracycline
Product Size: 20 µg

Contents & storage

Contains 20 µg of each: pcDNA™FRT⁄TO and pcDNA™FRT⁄TO⁄CAT vectors. Store both vectors at -20°C. All reagents are guaranteed stable for 6 months when properly stored.

Documents

Manuals & protocols