Invitrogen™

Vybrant™ Phagocytosis Assay Kit

Catalog number: V6694
Invitrogen™

Vybrant™ Phagocytosis Assay Kit

Catalog number: V6694
Catalog Number
V6694
also known as V-6694
Unit Size
1 kit
Price (USD)
394.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
V6694
also known as V-6694
1 kit
394.00
Your Price:
***
Product Overview
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The Vybrant® Phagocytosis Assay Kit provides a way for researchers to observe and quantitate the process of phagocytosis in human polynuclear cells and mouse macrophages by following the internalization of a foreign particle—in this case killed E. coli (K-12 strain) cells that have been labeled with the fluorescent dye fluorescein. In addition to the lyophilized E. coli BioParticles® component, the kit contains a trypan blue solution (to quench the fluorescence from particles that were not internalized) as well as step-by-step instructions for performing this phagocytosis assay in a fluorescence microplate reader. The methodology used with this kit has been developed using an adherent murine macrophage cell line (J774); however, by modifying the cell culture conditions, researchers can adapt this phagocytosis assay to other adherent cell lines.

Vybrant® Phagocytosis Assay Kit Specifications:
• Particles are fluorescein-labeled E. coli (K-12 strain)
• Kit supplies sufficient reagents for ∼250 tests using 96-well plates
• Fluorescence is measured using a microplate reader (Ex/Em ∼480/520 nm)


Find More BioParticles® Products
We offer a large range of dye-labeled and unlabeled E. coli (K-12 strain), S. aureus (Wood strain, without protein A), and zymosan (S. cerevisiae) BioParticles® products. Find out about these products and their applications by reviewing Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes® Handbook.

For pH-sensitive endocytosis assays, see our pHrodo™ BioParticles® conjugates.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.
WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Species
E. coli
Detection Method
Fluorescent
Product Type
Phagocytosis Assay Kit
For Use With (Equipment)
Fluorescence Microscope, Microplate Reader
Label Type
Classic Dyes
Label or Dye
FITC (Fluorescein)
Shipping Condition
Room Temperature
Product Line
Vybrant™
Format
96-well plate

Contents & Storage

Store in freezer (-5 to -30°C) and protect from light.

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