The Vybrant® Phagocytosis Assay Kit provides a way for researchers to observe and quantitate the process of phagocytosis in human polynuclear cells and mouse macrophages by following the internalization of a foreign particle—in this case killed E. coli
(K-12 strain) cells that have been labeled with the fluorescent dye fluorescein. In addition to the lyophilized E. coli
BioParticles® component, the kit contains a trypan blue solution (to quench the fluorescence from particles that were not internalized) as well as step-by-step instructions for performing this phagocytosis assay in a fluorescence microplate reader. The methodology used with this kit has been developed using an adherent murine macrophage cell line (J774); however, by modifying the cell culture conditions, researchers can adapt this phagocytosis assay to other adherent cell lines.Vybrant® Phagocytosis Assay Kit Specifications:
• Particles are fluorescein-labeled E. coli (K-12 strain)
• Kit supplies sufficient reagents for ∼250 tests using 96-well plates
• Fluorescence is measured using a microplate reader (Ex/Em ∼480/520 nm)Find More BioParticles® Products
We offer a large range of dye-labeled and unlabeled E. coli
(K-12 strain), S. aureus
(Wood strain, without protein A), and zymosan (S. cerevisiae
) BioParticles® products. Find out about these products and their applications by reviewing Probes for Following Receptor Binding and Phagocytosis—Section 16.1
in the Molecular Probes® Handbook.
For pH-sensitive endocytosis assays, see our pHrodo™ BioParticles® conjugates
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.