Invitrogen™
pIZ/V5-His Vector Kit
Error loading your content!
| Catalog number |
Select a plan
Unit size
|
Price ({{currency}}) | Availability | Qty | |
|---|---|---|---|---|---|
| {{product.sku}} {{product.sku}} also known as {{product.formattedSku}} |
|
— |
Pro add-ons
Your on-site stock
››
{{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
››
{{supplyCenter.scName}}(Out of stock)
››
{{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Description
The InsectSelect™ Kit features the pIZ/V5-His vector for high-level expression in a variety of insect cells. This exceptionally small vector has several features that facilitate expression, analysis, and detection of recombinant protein in insect cells:• The OpIE2 promoter for constitutive expression
• The Zeocin™ resistance gene for rapid selection of stably transfected cell lines
• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification using nickel-chelating resin
For Research Use Only. Not for use in diagnostic procedures.
Figures
Specifications
| Cloning Method: | Restriction Enzyme ⁄ MCS |
|---|---|
| Product Line: | InsectSelect™ |
| Product Size: | 1 kit |
| Promoter: | OplE2 |
| Protein Tag Position (to your gene): | C-terminal |
| Protein Tag or Fusion: | His Tag (6x), V5 Epitope Tag |
| Vector Type: | pIZ |
Contents & storage
The InsectSelect™ Kit includes 20 µg each of supercoiled, lyophilized pIZ/V5-His and pIZ/V5-His/CAT, 2 µg of lyophilized OpIE2 Reverse Sequencing primer, 1 g Zeocin™, Cellfectin® Reagent, and 1 L of medium for the insect cell line chosen. Sf9 cells are supplied with GIBCO™ Grace's Insect Media, High Five™ Cells are supplied with GIBCO™ Express Five® Serum-Free Medium. The pIZ/V5-His Vector Kit contains only the expression vectors and primer as described above. Store frozen insect cells in liquid nitrogen. Store medium and Cellfectin® Reagent at +4°C. Store all other reagents at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
