pIZ/V5-His Vector Kit

Catalog number: V800001

Invitrogen™  Related applications: Protein Expression

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Description

The InsectSelect™ Kit features the pIZ/V5-His vector for high-level expression in a variety of insect cells. This exceptionally small vector has several features that facilitate expression, analysis, and detection of recombinant protein in insect cells:

• The OpIE2 promoter for constitutive expression
• The Zeocin™ resistance gene for rapid selection of stably transfected cell lines
• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification using nickel-chelating resin
For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Promoter: OplE2
Vector Type: pIZ
Product Size: 1 kit
Cloning Method: Restriction Enzyme ⁄ MCS
Protein Tag or Fusion: His Tag (6x), V5 Epitope Tag
Protein Tag Position (to your gene): C-terminal

Contents & storage

The InsectSelect™ Kit includes 20 µg each of supercoiled, lyophilized pIZ/V5-His and pIZ/V5-His/CAT, 2 µg of lyophilized OpIE2 Reverse Sequencing primer, 1 g Zeocin™, Cellfectin® Reagent, and 1 L of medium for the insect cell line chosen. Sf9 cells are supplied with GIBCO™ Grace's Insect Media, High Five™ Cells are supplied with GIBCO™ Express Five® Serum-Free Medium. The pIZ/V5-His Vector Kit contains only the expression vectors and primer as described above. Store frozen insect cells in liquid nitrogen. Store medium and Cellfectin® Reagent at +4°C. Store all other reagents at -20°C. All reagents are guaranteed stable for 6 months when properly stored.

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Manuals & protocols