pYES2/CT Yeast Expression Vector

Catalog number: V825120

Invitrogen™  Related applications: Protein Expression

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Description

pYES2/NT and pYES2/CT are S. cerevisiae expression vectors derived from the parental pYES2 vector. Both vectors offer the URA3 gene for selection in yeast. pYES2/NT features an N-terminal Xpress™ epitope for detection with Invitrogen's Anti-Xpress™ Antibody and a polyhistidine (6xHis) tag for purification with nickel-chelating resin. pYES2/CT contains a C-terminal V5 epitope for detection and a polyhistidine (6xHis) tag for purification.
For Research Use Only. Not for use in diagnostic procedures.

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Specifications

Antibiotic Resistance (Bacterial): Ampicillin (AmpR)
Cloning Method: Restriction Enzyme ⁄ MCS
Product Size: 20 µg
Promoter: GAL1
Protein Tag or Fusion: His Tag (6x), V5 Epitope Tag
Vector Type: pYES

Contents & storage

Vectors are supplied supercoiled and lyophilized. pYES2/NT is provided in three reading frames. Both pYES/NT and pYES/CT include a lacZ expression control vector and an INVSc1 yeast stab. Store vectors at -20°C. Store the stab at +4°C. All reagents are guaranteed stable for 6 months when properly stored.

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