pShooter™ Mammalian Expression Vector (pEF/myc/nuc)

Catalog number: V89120

Invitrogen™  Related applications: Cell Structure | Protein Expression

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The pShooter™ vectors are designed to localize recombinant proteins to the nucleus, mitochondria, endoplasmic reticulum, or cytoplasm. In addition to the localization signal, each pShooter™ vector contains:

• Strong mammalian promoter (CMV or EF-1É) for constitutive expression of your protein of interest
• C-terminal c-myc epitope for detection with an Anti-myc Antibody
• Bovine Growth Hormone (BGH) polyadenylation site for mRNA stability
• f1 origin for single-stranded rescue of sense DNA
• Neomycin resistance gene for selection of mammalian cells with Geneticin¤ selection agent
• Ampicillin resistance and pUC origin for selection and maintenance in E. coli

Each vector is provided with a positive control plasmid. The positive control expresses GFP and targets it to the same subcellular location as the pShooter™ vector.
For Research Use Only. Not for use in diagnostic procedures.



Promoter: EF-1α
Key Function: Subcellular Localization
Protein Tag or Fusion: c-Myc Epitope Tag
Delivery Method: Transfection
Cloning Method: Restriction Enzyme ⁄ MCS
Selection Agent (Eukaryotic): Geneticin® (G-418)
Vector Type: pEF
Product Size: 20 µg
Constitutive or Inducible System: Constitutive

Contents & storage

20 µg of the pShooter™ vector and GFP control are supplied supercoiled and lyophilized. Store at -20°C. Vectors are guaranteed stable for 6 months when properly stored.


Manuals & protocols