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Varioskan™ ALF Multimode Microplate Reader
The Thermo Scientific Varioskan ALF Multimode Microplate Reader is a versatile, entry level reader for most common absorbance, turbidimetric, fluorescence, and luminescence microplate assays.
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| Catalog Number | Warranty |
|---|---|
| VA000010C | 1-year Standard |
| A66711 | 2-year Rapid Exchange |
Catalog number VA000010C
Price (USD)
19,790.00
1 each
Warranty:
1-year Standard
Price (USD)
19,790.00
1 each
The Thermo Scientific Varioskan ALF Multimode Microplate Reader is a versatile, entry level reader for most common absorbance, turbidimetric, fluorescence, and luminescence microplate assays. Its photometric optical design is based on a xenon flash lamp and monochromator, enabling continuous wavelength selection from UV through visible range up to 1000 nm. The broad wavelength range enables many common assays, including nucleic acid and protein quantification, bacterial growth curves, ELISA, and cell viability.
Enhance your fluorescence and luminescence measurements with the filter-based optics
With the Varioskan ALF reader, fluorescence and luminescence measurements are performed with filter-based optics for optimal specificity and sensitivity. The system comes with three pre-installed excitation and emission filter sets suitable for many common fluorometric applications like Quant-IT DNA quantitation assays and PrestoBlue and AlamarBlue cell viability assays. Additionally, five open filter positions are available for customized fluorometric and luminometric measurements. The filter slots are easy to access on the instrument's side with no tools required for filter exchange. Also, commonly used FRET and BRET assays are easy to set up and perform thanks to Varioskan ALF reader's unique filter-based optics design.
This package includes a Varioskan ALF Multimode Microplate Reader (Cat. No. VA000010C) and a Rapid Exchange two-year extended warranty.
Achieve consistently accurate results with automatic gain adjustment
The automatic gain adjustment feature selects the ideal fluorometric or luminometric reading range for your instrument based on signal intensity in the well, eliminating the need to manually adjust measurement parameters. The results are consistent no matter what signals are measured.
Additional features
- Kinetic and endpoint measurements for a variety of applications
- Spectral scanning for photometric measurements
- Separate optimized measurement modes for absorbance and turbidimetric measurements
- A self-diagnostics system that includes a set of initialization tests and adjustments, ensuring instrument readiness for operation
- Compatible with 6- to 384-well microplates with and without lids
- Reads μDrop and μDrop Duo plates for microvolume analysis of DNA, RNA, and proteins
- Performs onboard shaking and incubation for temperature-critical assays
- Operated through SkanIt software
- Unlimited installation on multiple computers, no annual fee required
- Includes an extensive cloud-based library of ready-made protocols
- Two editions available: Research Edition for life sciences and Drug Discovery Edition with features for compliance with 21 CFR Part 11
- Multiple user interface languages available
- Automation compatibility for integration with automation interface
- Thermo Scientific verification plates provide comprehensive and traceable validation of the instrument optical performance. Ready-made SkanIt software protocols provide easy to read reports with defined measurement parameter limits
Varioskan ALF Multimode Microplate Reader with 2-year Extended Warranty (A66711), REX is a Varioskan ALF specific package SKU including the instrument (VA000010C), and an extended warranty to 2 years which includes a rapid exchange service (REX) should the instrument get broken during the warranty period.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Absorbance Range200 to 1000 nm
Accuracy (Photometric)±1.0% + 0.003 Abs (0 to 2 Abs), ±2.0% (2.0 to 2.5 Abs) at 450 nm (96-well plate)
BandwidthPhotometry: 2.5 nm
Depth (Metric)61 cm
Dimensions (L x W x H)61 x 34 x 40 cm
Dynamic RangeFluorometry and Luminometry: ≥6 Decades
Emission Wavelength Range210-720 nm
Excitation Wavelength Range200-710 nm
FiltersThree factory installed fluorometric filter pairs: Coumarin family (Excitation 345/25 nm, Emission 450/40 nm); Fluorescein family (Excitation 485/15 nm, Emission 525/15 nm); Resorufin family (Excitation 555/15 nm, Emission 615/45 nm)
For Use With (Application)Photometric, fluorometric and luminometric microplate assays
Height (Metric)40 cm
IncludesSkanIt software, Research Edition
Linearity0 to 2.5 Abs, 2.0% at 450 nm (96-well plate)
Measurement SpeedPhotometry: ≤10 sec. (fast mode); Fluorometry and Luminometry: ≤20 sec. (96-well plate)
PrecisionSD <0.003 Abs, or CV <1.0% at 450 nm
Product TypeMultimode Microplate Reader
QuantityMicroplate Reader + 1 year warranty
SensitivityPhotometry (DNA): <1 ng/μL (96-well half area plate); Turbidimetry (formazin): <5 turbidity units (FTUs) (96-well plate); Fluorometry (fluorescein): 0.35 fmol/well (black 384-well plate); Luminometry (ATP): <275 amol/well (white 96-well plate)
Software Language VersionsEnglish, French, German, Spanish, Portuguese, Italian, Japanese, Chinese
Spectral Scanning Speed≤16 sec. (Fast mode)
Voltage100/115/220/230 V
Warranty1-year Standard
Weight (Metric)24.5 kg
Width (Metric)34 cm
CuvettesNo
Detector TypePhotomultiplier Tube (PMT)
Incubator TemperatureAmbient +2° to 45°C
InterfacePC Software (Thermo Scientific SkanIt Software)
Light SourceXenon Flash Lamp
Product LineVarioskan
Reading OptionsEndpoint, Kinetic
ShakingLinear, Orbital, Double Orbital
Software TypeSkanIt Software
Unit Size1 each