Donkey anti-Goat IgG (H+L) Secondary Antibody, WesternDot™ 585 - FAQs

View additional product information for Donkey anti-Goat IgG (H+L) Secondary Antibody, WesternDot™ 585 - FAQs (W10802)

3 product FAQs found

Is it possible to do a total protein stain of the blot before WesternDot labeling?

Yes. You can perform Ponceau staining for total protein. We recommend washing the blot to remove the Ponceau stain prior to applying the WesternDot label.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

With WesternDot reagents, when should I not use non-fat dry milk as a blocking reagent?

Non-fat dry milk contains biotin and a high content of phosphoproteins and its composition may vary considerably from batch to batch. Avoid using non-fat dry milk if using streptavidin conjugates and when probing phosphoproteins. If you are observing high background or poor resolution, it is advisable to use either highly purified casein, a chemically-defined blocking reagent, or a non-protein blocker.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is it possible to probe all four WesternDot fluorescent colors on a single blot?

Yes. All four WesternDot quantum dots may be excited from a single wavelength (such as UV light or blue light) and detected with emission filters suitable for each fluorophore. Refer to Table 2 of the product manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/westerndot_secondary_ab_conj_man.pdf) for recommendations on suitable filter sets for each instrument.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.