Single-molecule level analysis of the subunit composition of the T cell receptor on live T cells.
AuthorsJames JR, White SS, Clarke RW, Johansen AM, Dunne PD, Sleep DL, Fitzgerald WJ, Davis SJ, Klenerman D,
JournalProc Natl Acad Sci U S A
PubMed ID17971442
'The T cell receptor (TCR) expressed on most T cells is a protein complex consisting of TCRalphabeta heterodimers that bind antigen and cluster of differentiation (CD) 3epsilondelta, epsilongamma, and zetazeta dimers that initiate signaling. A long-standing controversy concerns whether there is one, or more than one, alphabeta heterodimer per complex. ... More
Phosphoglycerate mutase affects Stenotrophomonas maltophilia attachment to biotic and abiotic surfaces.
AuthorsRamos-Hegazy L, Chakravarty S, Anderson GG
Journal
PubMed ID31430538
Stenotrophomonas maltophilia biofilm formation is of increasing medical concern, particularly for lung infections. However, the molecular mechanisms facilitating the biofilm lifestyle in S. maltophilia are poorly understood. We generated and screened a transposon mutant library for mutations that lead to altered biofilm formation compared to wild type. One of these mutations, ... More
Novel role of sorting nexin 5 in renal D1 dopamine receptor trafficking and function: implications for hypertension.
AuthorsVillar VA, Armando I, Sanada H, Frazer LC, Russo CM, Notario PM, Lee H, Comisky L, Russell HA, Yang Y, Jurgens JA, Jose PA, Jones JE,
JournalFASEB J
PubMed ID23195037
The D1 dopamine receptor (D1R) is widely expressed in the kidney and plays a crucial role in blood pressure regulation. Although much is known about D1R desensitization, especially through G-protein-coupled receptor kinase 4 (GRK4), comparatively little is known about other aspects of D1R trafficking and the proteins involved in the ... More
Super-resolution imaging visualizes the eightfold symmetry of gp210 proteins around the nuclear pore complex and resolves the central channel with nanometer resolution.
AuthorsLöschberger A, van de Linde S, Dabauvalle MC, Rieger B, Heilemann M, Krohne G, Sauer M,
JournalJ Cell Sci
PubMed ID22389396
One of the most complex molecular machines of cells is the nuclear pore complex (NPC), which controls all trafficking of molecules in and out of the nucleus. Because of their importance for cellular processes such as gene expression and cytoskeleton organization, the structure of NPCs has been studied extensively during ... More
Mouse and human resistins impair glucose transport in primary mouse cardiomyocytes, and oligomerization is required for this biological action.
AuthorsGraveleau C, Zaha VG, Mohajer A, Banerjee RR, Dudley-Rucker N, Steppan CM, Rajala MW, Scherer PE, Ahima RS, Lazar MA, Abel ED
JournalJ Biol Chem
PubMed ID15983036
The adipocytokine resistin impairs glucose tolerance and insulin sensitivity in rodents. Here, we examined the effect of resistin on glucose uptake in isolated adult mouse cardiomyocytes. Murine resistin reduced insulin-stimulated glucose uptake, establishing the heart as a resistin target tissue. Notably, human resistin also impaired insulin action in mouse cardiomyocytes, ... More
A novel celecoxib analog UTX-121 inhibits HT1080 cell invasion by modulating membrane-type 1 matrix metalloproteinase.
AuthorsYamahana H, Takino T, Endo Y, Yamada H, Suzuki T, Uto Y
JournalBiochem Biophys Res Commun
PubMed ID31629465
'We designed and synthesized a celecoxib derivative UTX-121 to enhance its anti-tumor activity. Similar to celecoxib, this compound could also inhibit matrix metalloproteinase (MMP)-9 activity. In addition, UTX-121 suppressed membrane-type 1 MMP (MT1-MMP)-mediated pro-MMP-2 activation by disturbing the cell surface expression of MT1-MMP. UTX-121 also impeded the glycosylation of cell ... More
AuthorsHe J, Li T, Próchnicki T, Horvath G, Latz E, Takeoka S
JournalBiochem Biophys Rep
PubMed ID31011633
'Lysine (K) type cationic lipid with a propyl spacer and ditetradecyl hydrophobic moieties composing liposomes, K3C14, previously studied for gene delivery, were reported to activate the NLRP3 inflammasomes in human macrophages via the conventional phagolysosomal pathway. In this study, K3C16, a propyl spacer bearing lysine type lipids with dihexadecyl moieties ... More
A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol.
Authors
JournalNature
PubMed ID32132706
Super-resolution imaging of multiple cells by optimised flat-field epi-illumination.
Authors
JournalNat Photonics
PubMed ID27818707
Multiplexed Proteome Dynamics Profiling Reveals Mechanisms Controlling Protein Homeostasis.
Authors
JournalCell
PubMed ID29551266
Tissue-Specific Macrophage Responses to Remote Injury Impact the Outcome of Subsequent Local Immune Challenge.
Authors
JournalImmunity
PubMed ID31732166
Nuclear Pores Assemble from Nucleoporin Condensates During Oogenesis.
Traction forces at the cytokinetic ring regulate cell division and polyploidy in the migrating zebrafish epicardium.
Authors
JournalNat Mater
PubMed ID31160803
A Unique Collateral Artery Development Program Promotes Neonatal Heart Regeneration.
Authors
JournalCell
PubMed ID30686582
Obesity-associated hyperleptinemia alters the gliovascular interface of the hypothalamus to promote hypertension.
Authors
JournalCell Metab
PubMed ID33951475
Transmembrane Pickets Connect Cyto- and Pericellular Skeletons Forming Barriers to Receptor Engagement.
Authors
JournalCell
PubMed ID29328918
A simple and robust fluorescent labeling method to quantify trophoblast fusion.
AuthorsZhang Y, Yang H
JournalPlacenta
PubMed ID30827351
Trophoblast fusion into syncytiotrophoblasts is a specialized yet enigmatic cellular process, which is essential for placental development and function. To facilitate mechanistic understanding of this critical process, here we re-purposed a widely used fluorescent membrane potential dye, Di-8-ANEPPS, to stably label the plasma membrane of live BeWo trophoblast cells. Compared ... More
Dynamic Transcriptional Responses to Injury of Regenerative and Non-regenerative Cardiomyocytes Revealed by Single-Nucleus RNA Sequencing.
AuthorsCui M, Wang Z, Chen K, Shah AM, Tan W, Duan L, Sanchez-Ortiz E, Li H, Xu L, Liu N, Bassel-Duby R, Olson EN
JournalDev Cell
PubMed ID32220304
The adult mammalian heart is incapable of regeneration following injury. In contrast, the neonatal mouse heart can efficiently regenerate during the first week of life. The molecular mechanisms that mediate the regenerative response and its blockade in later life are not understood. Here, by single-nucleus RNA sequencing, we map the ... More
Glycine Enhances Satellite Cell Proliferation, Cell Transplantation, and Oligonucleotide Efficacy in Dystrophic Muscle.
AuthorsLin C, Han G, Ning H, Song J, Ran N, Yi X, Seow Y, Yin H
JournalMol Ther
PubMed ID32209436
The need to distribute therapy evenly systemically throughout the large muscle volume within the body makes Duchenne muscular dystrophy (DMD) therapy a challenge. Cell and exon-skipping therapies are promising but have limited effects, and thus enhancing their therapeutic potency is of paramount importance to increase the accessibility of these therapies ... More
Group A Streptococcus establishes pharynx infection by degrading the deoxyribonucleic acid of neutrophil extracellular traps.
AuthorsTanaka M, Kinoshita-Daitoku R, Kiga K, Sanada T, Zhu B, Okano T, Aikawa C, Iida T, Ogura Y, Hayashi T, Okubo K, Kurosawa M, Hirahashi J, Suzuki T, Nakagawa I, Nangaku M, Mimuro H
JournalSci Rep
PubMed ID32094510
Group A Streptococcus (GAS) secretes deoxyribonucleases and evades neutrophil extracellular killing by degrading neutrophil extracellular traps (NETs). However, limited information is currently available on the interaction between GAS and NETs in the pathogenicity of GAS pharyngitis. In this study, we modified a mouse model of GAS pharyngitis and revealed an ... More
Ovine Herpesvirus 2 Encodes a Previously Unrecognized Protein, pOv8.25, That Targets Mitochondria and Triggers Apoptotic Cell Death.
AuthorsShrestha N, Tobler K, Uster S, Sigrist-Nagy R, Hierweger MM, Ackermann M
JournalJ Virol
PubMed ID32024777
Malignant catarrhal fever (MCF) is a rare but frequently lethal disease of certain cloven-hoofed animals. At least 10 different viruses, all members of the
The HopQ-CEACAM Interaction Controls CagA Translocation, Phosphorylation, and Phagocytosis of
AuthorsBehrens IK, Busch B, Ishikawa-Ankerhold H, Palamides P, Shively JE, Stanners C, Chan C, Leung N, Gray-Owen S, Haas R
JournalmBio
PubMed ID32019805
The
Skeletal muscle lipid droplets are resynthesized before being coated with perilipin proteins following prolonged exercise in elite male triathletes.
AuthorsJevons EFP, Gejl KD, Strauss JA, Ørtenblad N, Shepherd SO
JournalAm J Physiol Endocrinol Metab
PubMed ID31935113
Intramuscular triglycerides (IMTG) are a key substrate during prolonged exercise, but little is known about the rate of IMTG resynthesis in the postexercise period. We investigated the hypothesis that the distribution of the lipid droplet (LD)-associated perilipin (PLIN) proteins is linked to IMTG storage following exercise. Fourteen elite male triathletes ... More
Multinucleated polyploid cardiomyocytes undergo an enhanced adaptability to hypoxia via mitophagy.
AuthorsJiang YH, Wang HL, Peng J, Zhu Y, Zhang HG, Tang FQ, Jian Z, Xiao YB
JournalJ Mol Cell Cardiol
PubMed ID31783035
There is a large subpopulation of multinucleated polyploid cardiomyocytes (M*Pc CMs) in the adult mammalian heart. However, the pathophysiological significance of increased M*Pc CMs in heart disease is poorly understood. We sought to determine the pathophysiological significance of increased M*Pc CMs during hypoxia adaptation. ... More