Flow cytometric analysis of human erythrocytes: II. Possible identification of senescent RBC with fluorescently labelled wheat germ agglutinin.
AuthorsRolfes-Curl A, Ogden LL, Omann GM, Aminoff D
JournalExp Gerontol
PubMed ID1936192
In the first paper of a series (Gutowski, et al., 1991) we discussed the use of flow cytometry to follow at the cellular level the aging of red blood cells (RBC) in circulation, using fluorescently labelled lectins and goat anti-human-IgG and -IgM. The Coulter Epics 541 was used for those ... More
Direct priming of antiviral CD8+ T cells in the peripheral interfollicular region of lymph nodes.
'It is uncertain how antiviral lymphocytes are activated in draining lymph nodes, the site where adaptive immune responses are initiated. Here, using intravital microscopy we show that after infection of mice with vaccinia virus (a large DNA virus) or vesicular stomatitis virus (a small RNA virus), virions drained to the ... More
Lipid-dependent bidirectional traffic of apolipoprotein B in polarized enterocytes.
AuthorsMorel E, Demignot S, Chateau D, Chambaz J, Rousset M, Delers F
JournalMol Biol Cell
PubMed ID14565984
'Enterocytes are highly polarized cells that transfer nutrients across the intestinal epithelium from the apical to the basolateral pole. Apolipoprotein B (apoB) is a secretory protein that plays a key role in the transepithelial transport of dietary fatty acids as triacylglycerol. The evaluation of the control of apoB traffic by ... More
Synaptic vesicle proteins and early endosomes in cultured hippocampal neurons: differential effects of Brefeldin A in axon and dendrites.
AuthorsMundigl O, Matteoli M, Daniell L, Thomas-Reetz A, Metcalf A, Jahn R, De Camilli P
JournalJ Cell Biol
PubMed ID8376458
'The pathways of synaptic vesicle (SV) biogenesis and recycling are still poorly understood. We have studied the effects of Brefeldin A (BFA) on the distribution of several SV membrane proteins (synaptophysin, synaptotagmin, synaptobrevin, p29, SV2 and rab3A) and on endosomal markers to investigate the relationship between SVs and the membranes ... More
Immunological evidence for methylglyoxal-derived modifications in vivo. Determination of antigenic epitopes.
AuthorsShamsi FA, Partal A, Sady C, Glomb MA, Nagaraj RH
JournalJ Biol Chem
PubMed ID9506998
'The Maillard reaction, a non-enzymatic reaction of ketones and aldehydes with amino groups of proteins, contributes to the aging of proteins and to complications associated with diabetes. Methylglyoxal (MG) is a 2-oxoaldehyde derived from glycolytic intermediates and produced during the Maillard reaction. We reported previously the formation of a lysine-lysine ... More
Interaction of a neuron-specific protein containing PDZ domains with Alzheimer's amyloid precursor protein.
AuthorsTomita S, Ozaki T, Taru H, Oguchi S, Takeda S, Yagi Y, Sakiyama S, Kirino Y, Suzuki T
JournalJ Biol Chem
PubMed ID9890987
'A novel protein, human X11-like (human X11L), contains a phosphotyrosine interaction (PI) domain and two PDZ domains and displays 55.2% amino acid homology with the human X11 (human X11). The PI domain of human X11L interacts with a sequence containing the NPXY motif found in the cytoplasmic domain of Alzheimer''s ... More
Role of the Gag matrix domain in targeting human immunodeficiency virus type 1 assembly.
AuthorsOno A, Orenstein JM, Freed EO
JournalJ Virol
PubMed ID10684302
'Human immunodeficiency virus type 1 (HIV-1) particle formation and the subsequent initiation of protease-mediated maturation occur predominantly on the plasma membrane. However, the mechanism by which HIV-1 assembly is targeted specifically to the plasma membrane versus intracellular membranes is largely unknown. Previously, we observed that mutations between residues 84 and ... More
Acetylcholine receptor aggregation parallels the deposition of a basal lamina proteoglycan during development of the neuromuscular junction.
AuthorsAnderson MJ, Klier FG, Tanguay KE
JournalJ Cell Biol
PubMed ID6386827
'To determine the time course of synaptic differentiation, we made successive observations on identified, nerve-contacted muscle cells developing in culture. The cultures had either been stained with fluorescent alpha-bungarotoxin, or were maintained in the presence of a fluorescent monoclonal antibody. These probes are directed at acetylcholine receptors (AChR) and a ... More
A direct fluorescence technique for the rapid detection of sheathed microfilariae in blood smears.
AuthorsRao UR, Lowrie RC, Vickery AC, Kwa BH, Nayar JK
JournalInt J Parasitol
PubMed ID2074139
'Thick and thin blood smears containing microfilariae of Wuchereria bancrofti, Loa loa, Brugia malayi, Brugia pahangi, Brugia patei or Acanthocheilonema viteae were prepared from either cryopreserved blood samples or from freshly collected blood, fixed in methanol and treated with a fluoresceinated lectin wheat germ agglutinin. Sheathed microfilariae of W. bancrofti, ... More
Immunofluorescent quantification of ribonucleotide reductase M1 subunit and correlation with DNA content by flow cytometry.
AuthorsMann GJ, Dyne M, Musgrove EA
JournalCytometry
PubMed ID3311659
'The cytoplasmic enzyme ribonucleotide reductase is essential for DNA synthesis, and its activity is strongly correlated with cellular proliferation. This paper describes a flow cytometric technique for the simultaneous measurement of DNA content and the M1 subunit of ribonucleotide reductase. Data are presented for cycling cultured human leukemic lymphoblasts in ... More
Fluorescence-labeled lectins, glycoconjugates, and the development of the mouse AOP.
AuthorsVanden Hoek TL, Goossens W, Knepper PA
JournalInvest Ophthalmol Vis Sci
PubMed ID3557857
'The development of the aqueous outflow pathway (AOP) in early postnatal mouse eyes was examined for the presence of a variety of lectin receptors using fluorescein isothiocyanate (FITC) conjugated lectins, 1 micron araldite plastic sections, and computer-aided fluorescence photography. The trabecular meshwork anlage (days 1-4) was characterized by the presence ... More
Surface carbohydrates of procyclic forms of African trypanosomes studied using fluorescence activated cell sorter analysis and agglutination with lectins.
AuthorsMutharia LM, Pearson TW
JournalMol Biochem Parasitol
PubMed ID3106807
'Living culture form procyclics of Trypanosoma brucei brucei, T.b. rhodesiense, T.b. gambiense, T. congolense and T. simiae were tested for binding of eight different lectins. The binding of fluorescein isothiocyanate (FITC)-conjugated lectins was measured using a fluorescence activated cell sorter (FACS) and by agglutination with unlabelled lectins. Five of the ... More
ADP-ribosylation factor 6 regulates a novel plasma membrane recycling pathway.
AuthorsRadhakrishna H, Donaldson JG
JournalJ Cell Biol
PubMed ID9314528
'ADP-ribosylation factor (ARF) 6 localizes to the plasma membrane (PM) in its GTP state and to a tubulovesicular compartment in its GDP state in HeLa cells that express wild-type or mutant forms of this GTPase. Aluminum fluoride (AlF) treatment of ARF6-transfected cells redistributes ARF6 to the PM and stimulates the ... More
Fluorochrome-coupled lectins reveal distinct cellular domains in human epidermis.
AuthorsVirtanen I, Kariniemi AL, Holthöfer H, Lehto VP
JournalJ Histochem Cytochem
PubMed ID3512692
'The distribution of saccharide moieties in human interfollicular epidermis was studied with fluorochrome-coupled lectins. In frozen sections Concanavalin A (Con A), Lens culinaris agglutinin (LCA), Ricinus communis agglutinin I (RCAI), and wheat germ agglutinin (WGA) stained intensively both dermis and viable epidermal cell layers, whereas peanut agglutinin (PNA) bound only ... More
Lateral diffusion of wheat germ agglutinin-labeled glycoconjugates in the membrane of differentiating HL-60 and U-937 cells assessed with fluorescence recovery after photobleaching (FRAP).
AuthorsMagnusson KE, Wojcieszyn J, Dahlgren C, Stendahl O, Sundqvist T, Jacobson K
JournalCell Biophys
PubMed ID6197174
'The promyelocytic leukemia cell line HL-60 and the histiocytic cell line U-937 were grown in suspension culture. They were induced to differentiate during 5-d cultivation in the presence of dimethylsulfoxide (DMSO; 1.3% w/v) or phorbol-12-myristate-acetate (PMA; 10(-7) M), which yields granulocyte- and macrophage-like cells, respectively. Differentiation was evidenced by increased ... More
Proline-rich motifs of the Na+/H+ exchanger 2 isoform. Binding of Src homology domain 3 and role in apical targeting in epithelia.
AuthorsChow CW, Woodside M, Demaurex N, Yu FH, Plant P, Rotin D, Grinstein S, Orlowski J
JournalJ Biol Chem
PubMed ID10187839
'The NHE2 isoform of the Na+/H+ exchanger (NHE) displays two proline-rich sequences in its C-terminal region that resemble SH3 (Src homology 3)-binding domains. We investigated whether these regions (743PPSVTPAP750, termed Pro-1, and 786VPPKPPP792, termed Pro-2) can bind to SH3 domains and whether they are essential for NHE2 function and targeting. ... More
Mutations of the Rous sarcoma virus env gene that affect the transport and subcellular location of the glycoprotein products.
AuthorsWills JW, Srinivas RV, Hunter E
JournalJ Cell Biol
PubMed ID6094591
'The envelope glycoproteins of Rous sarcoma virus (RSV), gp85 and gp37, are anchored in the membrane by a 27-amino acid, hydrophobic domain that lies adjacent to a 22-amino acid, cytoplasmic domain at the carboxy terminus of gp37. We have altered these cytoplasmic and transmembrane domains by introducing deletion mutations into ... More
Expression of several adhesive macromolecules (N-CAM, L1, J1, NILE, uvomorulin, laminin, fibronectin, and a heparan sulfate proteoglycan) in embryonic, adult, and denervated adult skeletal muscle.
AuthorsSanes JR, Schachner M, Covault J
JournalJ Cell Biol
PubMed ID3511069
'Levels of the neural cell adhesion molecule N-CAM in muscle are regulated in parallel with the susceptibility of muscle to innervation: N-CAM is abundant on the surface of early embryonic myotubes, declines in level as development proceeds, reappears when adult muscles are denervated or paralyzed, and is lost after reinnervation ... More
Lectin binding to newt epidermis: fluorescent localization and effects on motility.
AuthorsMahan JT, Donaldson DJ
JournalJ Exp Zool
PubMed ID3091758
'The ability of seven lectins to bind to newt epidermal cells and influence their motility was examined. Of the seven fluoresceinated lectins applied to frozen sections containing intact newt skin and migrating epidermis (wound epithelium), only Con A (concanavalin A), WGA (wheat germ agglutinin), and PNA (peanut agglutinin) produced detectable ... More
Lectin histochemistry of classic and spermatocytic seminoma.
AuthorsLee MC, Talerman A, Oosterhuis JW, Damjanov I
JournalArch Pathol Lab Med
PubMed ID3929746
'Six spermatocytic and 13 classic seminomas were studied histochemically with fluorescein isothiocyanate-labeled lectins. Wheat-germ lectin and succinyl concanavalin A reacted with all 19 tumors, whereas other lectins bound to some, but never to all, tumors in either group. Soybean lectin reacted with seven of 13 classic seminomas but with none ... More
Preparation of a high-affinity photolabeling reagent for the Gal/GalNAc lectin of mammalian liver: demonstration of galactose-combining sites on each subunit of rabbit hepatic lectin.
AuthorsLee RT, Lee YC
JournalBiochemistry
PubMed ID2432926
'On the basis of the knowledge that the D-galactose/N-acetyl-D-galactosamine-specific lectin of rabbit liver can tolerate a large group on the C-6 hydroxyl group of a galactoside [Lee, R. T. (1982) Biochemistry 21, 1045-1050], we prepared a high-affinity photolabeling reagent for this lectin from a triantennary glycopeptide fraction of asialofetuin. The ... More
Diminished calcium influx in lectin-stimulated T cells from old mice.
AuthorsMiller RA, Jacobson B, Weil G, Simons ER
JournalJ Cell Physiol
PubMed ID3497930
'T lymphocytes from aged donors function poorly, but the biochemical basis for the defect remains uncertain. We tested the hypothesis that T cells from old mice had a diminished ability to transmit extracellular signals into the cytoplasm, by measuring intracellular free calcium concentrations (Cai) in T cells stimulated by the ... More
Immunofluorescent localization of a 39,000-dalton substrate of tyrosine protein kinases to the cytoplasmic surface of the plasma membrane.
AuthorsNigg EA, Cooper JA, Hunter T
JournalJ Cell Biol
PubMed ID6304112
'The intracellular distribution of p39, a 39,000-dalton substrate for a number of tyrosine protein kinases, has been determined by indirect immunofluorescence microscopy. No binding of anti-p39 antibodies to intact cells was observed, indicating that this protein is not accessible to antibody on the cell surface. Following detergent permeabilization of formaldehyde-fixed ... More
Novel in vivo-degradable cellulose-chitin copolymer from metabolically engineered Gluconacetobacter xylinus.
AuthorsYadav V, Paniliatis BJ, Shi H, Lee K, Cebe P, Kaplan DL,
JournalAppl Environ Microbiol
PubMed ID20656868
'Despite excellent biocompatibility and mechanical properties, the poor in vitro and in vivo degradability of cellulose has limited its biomedical and biomass conversion applications. To address this issue, we report a metabolic engineering-based approach to the rational redesign of cellular metabolites to introduce N-acetylglucosamine (GlcNAc) residues into cellulosic biopolymers during ... More
Trichocyst membrane fate in living Paramecium primaurelia visualized by multimodal confocal imaging.
AuthorsRamoino P, Beltrame F, Diaspro A, Fato M
JournalEur J Cell Biol
PubMed ID9309393
'Trichocysts are secretory organelles located at the surface of several ciliates, docked at the plasma membrane. Their secretion is similar to other exocytic processes: the trichocyst membrane fuses with the plasma membrane, its content is released outside the cell, and the membrane is retrieved back into the cell. The fate ... More
Villin induces microvilli growth and actin redistribution in transfected fibroblasts.
AuthorsFriederich E, Huet C, Arpin M, Louvard D
JournalCell
PubMed ID2680107
'The function of villin, an actin-binding protein, has been investigated by transfecting fibroblasts with cloned human cDNAs encoding wild-type villin or functional villin domains. Synthesis of large amounts of villin induced the growth of numerous long microvilli on cell surfaces together with the redistribution of F-actin. These microvilli contained a ... More
Lectin receptors of rods and cones. Visualization by fluorescent label.
AuthorsBridges CD
JournalInvest Ophthalmol Vis Sci
PubMed ID6969714
'The binding of eight fluorescence-labeled lectins to visual cells was investigated. Concanavalin A (Con A) (specific for oligosaccharides containing mannose) bound evenly to the surfaces of rod outer segments (ROSs) from frog, cattle, goldfish, and turtle (Pseudemys). In frog, binding was observed on the inner segment, with intensifications at the ... More
Adhesion of glycosaminoglycan-deficient chinese hamster ovary cell mutants to fibronectin substrata.
AuthorsLeBaron RG, Esko JD, Woods A, Johansson S, Höök M
JournalJ Cell Biol
PubMed ID3346331
'We have examined the role of cell surface glycosaminoglycans in fibronectin-mediated cell adhesion by analyzing the adhesive properties of Chinese hamster ovary cell mutants deficient in glycosaminoglycans. The results of our study suggest that the absence of glycosaminoglycans does not affect the initial attachment and subsequent spreading of these cells ... More
Membrane changes in neural target cells studied with fluorescent lectin probes.
AuthorsGualandris L, Rougé P, Duprat AM
JournalJ Embryol Exp Morphol
PubMed ID6655431
'The competent ectoderm of Pleurodeles waltl comprises two cell layers with characteristic differences in their morphology, their composition and the molecular arrangement of the various constituents. The use of labelled lectin probes for observations of ectoderm tissue in vitro with u.v. microscopy (epi-illumination) and the quantification of the results show ... More
Photobleaching recovery studies of membrane events accompanying lectin stimulation of rabbit lymphocytes.
AuthorsLeuther MD, Barisas BG, Peacock JS, Krakauer H
JournalBiochem Biophys Res Commun
PubMed ID314290
A new class of model glycolipids: synthesis, characterization, and interaction with lectins.
Cytolocalization artifacts with immunofluorescent probes.
AuthorsFriedman SJ, Dewar CL, Skehan P
JournalBiochem Cell Biol
PubMed ID3552000
'Formaldehyde fixation, nonionic detergent extraction, and ligand binding are commonly used in conjunction with immunofluorescence microscopy to visualize antigens and lectin-reactive molecules in cytoskeletal preparations. These procedures have the potential to produce serious artifacts in cytolocalization studies, as is shown in the present investigation of wheat-germ agglutinin (WGA) binding and ... More
Rapid visualization of three common fungi using fluorescein-conjugated lectins.
AuthorsRobin JB, Arffa RC, Avni I, Rao NA
JournalInvest Ophthalmol Vis Sci
PubMed ID3514531
'The feasibility of using fluorescein-conjugated lectins to visualize and differentiate three fungi commonly involved in ophthalmic mycoses was evaluated. Using a panel of fluorescein-conjugated lectins, Candida albicans, Aspergillus fumigatus, and Fusarium solani were rapidly and reproducibly visualized in in vitro culture isolates, as well as in tissue samples and fixed ... More
Fluorescent antibodies and lectins stain intracellular structures in fixed cells treated with nonionic detergent.
AuthorsLaurila P, Virtanen I, Wartiovaara J, Stenman S
JournalJ Histochem Cytochem
PubMed ID207770
'Nonionic detergent (NP40) treatment of paraformaldehyde-fixed normal and SV40-transformed human fibroblasts resulted in intracellular penetration of two chosen fluorescent antibodies and Concanavalin A (Con A). After the detergent treatment nuclear SV40 T antigen, cytoplasmic fibronectin glycoprotein and Con A binding sites could be visualized in fluorescence microscopy. The lowest NP40 ... More
Protein blotting: detection of proteins with colloidal gold, and of glycoproteins and lectins with biotin-conjugated and enzyme probes.
AuthorsRohringer R, Holden DW
JournalAnal Biochem
PubMed ID2984955
'Methods to detect "native" proteins immobilized on nitrocellulose membranes in spot tests or on blots prepared from polyacrylamide slab gels after electrophoretic separation are described. Gold sols were found to be useful as general stains for proteins: They are polychromatic, yield an indelible record, and are complementary to india ink ... More
Use of lectins and polyethylene glycol for fusion of glycolipid-containing liposomes with eukaryotic cells.
AuthorsSzoka F, Magnusson KE, Wojcieszyn J, Hou Y, Derzko Z, Jacobson K
JournalProc Natl Acad Sci U S A
PubMed ID6940182
'Efficient fusion of phospholipid vesicles with monolayer cultures of eukaryotic cells was accomplished by attaching glycolipid-containing vesicles to the cell surface by using a lectin displaying binding for both the cell surface and the glycolipid, followed by treatment with polyethylene glycol. Fusion was inferred from the transfer of fluorescent lipid ... More
Flow cytometric analyses of lectin binding to rat alveolar macrophages.
AuthorsSaunders GC, Steinkamp JA, Lehnert BE
JournalCytometry
PubMed ID3123174
'The binding characteristics of ten FITC-labeled plant lectins (Con-A, MPA, BPA, PNA, WGA, SBA, UEA-I, DBA, GS-I, GS-II) to lavaged rat alveolar macrophages were assessed by flow cytometry. The alveolar macrophages (AM) were incubated with varying concentrations of each lectin in a pinocytosis-inhibiting buffer. In addition to measuring lectin-associated green ... More
Spectroscopic and microscopic studies of buffalo-bull (Bubalus bubalis) spermatozoa.
AuthorsBawa SR, Govil G, Dhingra MS, Werner G, Bains HK
JournalJ Submicrosc Cytol Pathol
PubMed ID8384930
'We have examined the epididymal (caput, corpus and cauda) and ejaculated spermatozoa of bufallo-bull (Bubalus bubalis) employing microscopic and spectroscopic techniques. Fluorescein isothiocyanate conjugated lectins namely concanavalin A (Con A), Dolichos biflorus (DBA), Maclura pomifera (MPA), peanut agglutinin (PNA), soybean agglutinin (SBA) and wheat germ agglutinin (WGA) were used to ... More
Lectins and heparin-binding features of human spermatozoa as analyzed by flow cytometry.
AuthorsVasquez JM, Magargee SF, Kunze E, Hammerstedt RH
JournalAm J Obstet Gynecol
PubMed ID2124086
'Sperm cell surface characteristics may serve as a useful diagnostic feature for the evaluation of seminal samples, but to date no useful analytical method(s) is available for their routine quantitative analysis. Fluorescein isothiocyanate-conjugated lectins, proteins with unique and specific saccharide-binding characteristics, and heparin are potentially useful markers for such an ... More
Axonal transport of lectins in the peripheral nervous system.
AuthorsBorges LF, Sidman RL
JournalJ Neurosci
PubMed ID6176698
'The binding and axonal transport of six lectins were studied in the peripheral nervous system of adult mice by an immunocytochemical method. After injection into muscle and subcutaneous sites, lectins known to bind preferentially N-acetylglucosamine or mannose sugars were transported axonally to ventral horn and dorsal root ganglion neurons. Twelve ... More
Monoclonal antibodies directed against protoplasts of soybean cells: analysis of the lateral mobility of plasma membrane-bound antibody MVS-1.
AuthorsMetcalf TN, Villanueva MA, Schindler M, Wang JL
JournalJ Cell Biol
PubMed ID2420806
'A monoclonal antibody (MVS-1) was used to monitor the lateral mobility of a defined component (Mr approximately 400,000) of the plasma membrane of soybean protoplasts prepared from suspension cultures of Glycine max (SB-1 cell line). The diffusion coefficient (D) of antibody MVS-1 bound to its target was determined (D = ... More
Endogenously expressed apolipoprotein E has different effects on cell lipid metabolism as compared to exogenous apolipoprotein E carried on triglyceride-rich particles.
'Apolipoprotein E (apoE) on model triglyceride-rich particles (TGRP) increases triglyceride (TG) utilization and cholesteryl ester (CE) hydrolysis, independent of its effect on enhancing particle uptake. We questioned whether, under physiological concentrations, endogenously expressed apoE has similar effects on cellular lipid metabolism as compared to exogenous apoE. J774 macrophages, which do ... More
Heterogeneous distribution of a basement membrane heparan sulfate proteoglycan in rat tissues.
AuthorsCouchman JR
JournalJ Cell Biol
PubMed ID2959669
'A heparan sulfate proteoglycan (HSPG) synthesized by murine parietal yolk sac (PYS-2) cells has been characterized and purified from culture supernatants. A monospecific polyclonal antiserum was raised against it which showed activity against the HSPG core protein and basement membrane specificity in immunohistochemical studies on frozen tissue sections from many ... More
Agglutination of isolated rod outer segments by lectins.
AuthorsBridges CD
JournalInvest Ophthalmol Vis Sci
PubMed ID6969711
'The interactions of ten lectins with rod outer segment (ROS) plasma membranes and the effects of various haptene sugars were examined by studying agglutination in suspensions of washed ROSs. It is concluded that agglutination sites on the ROS membrane involve receptors that are recognized by eight lectins with haptene sugar ... More
Apical localization of K+ channels in taste cells provides the basis for sour taste transduction.
AuthorsKinnamon SC, Dionne VE, Beam KG
JournalProc Natl Acad Sci U S A
PubMed ID2457924
'Previous studies have shown that mudpuppy taste receptor cells respond to sour taste stimuli (weak acids) with depolarizing receptor potentials or action potentials that are blocked by the K+ channel blocker tetraethylammonium. Voltage-clamp recordings from isolated taste cells indicated that taste receptor cells exhibit a variety of voltage-dependent conductances and ... More
Alternate gram staining technique using a fluorescent lectin.
AuthorsSizemore RK, Caldwell JJ, Kendrick AS
JournalAppl Environ Microbiol
PubMed ID1697149
'Fluorescence-labeled wheat germ agglutinin binds specifically to N-acetylglucosamine in the outer peptidoglycan layer of gram-positive bacteria. The peptidoglycan layer of gram-negative bacteria is covered by a membrane and is not labeled by the lectin. By exploiting this phenomenon, an alternative Gram staining technique has been developed.' ... More
Lectins facilitate calcium-induced fusion of phospholipid vesicles containing glycosphingolipids.
AuthorsDüzgünes N, Hoekstra D, Hong K, Papahadjopoulos D
JournalFEBS Lett
PubMed ID6745438
'Ca2+-induced fusion of phospholipid vesicles containing globoside (GL-4) or disialoganglioside (GDla) is several-fold slower than the fusion of the pure phospholipid vesicles. Lectins specific for these glycosphingolipids, soybean agglutinin and wheat germ agglutinin, respectively, enhance the rate of fusion when added to the vesicle suspension before the introduction of Ca2+. ... More
Controlled binding of liposomes to cultured cells by means of lectins.
AuthorsLiautard JP, Vidal M, Philippot JR
JournalCell Biol Int Rep
PubMed ID3841030
'Lectin-mediated binding of liposomes to Hela cells was analyzed as a function of different parameters. We show that the amount of lectin covalently bound to liposomes can be accurately controlled. We chose to work with 500 - to 1 000 molecules of WGA bound per liposome of 1 micron diameter. ... More
Application of fluorescently labelled lectins for the visualization and biochemical characterization of polysaccharides in biofilms of Pseudomonas aeruginosa.
AuthorsStrathmann M, Wingender J, Flemming HC
JournalJ Microbiol Methods
PubMed ID12031574
'Fluorescently labelled lectins were used in combination with epifluorescence microscopy and confocal laser scanning microscopy to allow the visualization and characterization of carbohydrate-containing extracellular polymeric substances (EPS) in biofilms of Pseudomonas aeruginosa. A mucoid strain characterized by an overproduction of the exopolysaccharide alginate, and an isogenic, non-mucoid strain were used. ... More
Histological, histochemical, and fine structural observations on the spleen of seals.
AuthorsSchumacher U, Welsch U
JournalAm J Anat
PubMed ID3661458
'Spleens of three species of Antarctic seals with different diving habits (Weddell seal, crabeater seal, and fur seal) have been studied with histological, histochemical, and electron microscopic methods. The spleens can be classified as nonsinusoidal, with capsule and trabeculae rich in innervated smooth muscle cells. The trabecular system is particularly ... More
'Nuclear pore proteins bearing O-linked N-acetylglucosamine (GlcNAc) are involved in nuclear transport, although a role for their glycosylation is not established. Xenopus egg extracts capable of reforming functional nuclei in vitro yielded nuclei with impaired transport and reduced nuclear pore density when depleted of wheat germ agglutinin-binding proteins. Many of ... More
Selective flow cytometric sorting of viable dopamine neurons.
'Flow cytometry has been revealed as a powerful technique for studying cell populations. The availability of the method to identify, analyze and isolate specific populations of central nervous system cells will be of great aid for studying the in vivo (i.e. neural transplants) and in vitro (i.e. cell cultures) behavior ... More
The carbohydrate moiety of rhodopsin: lectin-binding, chemical modification and fluorescence studies.
AuthorsRenthal R, Steinemann A, Stryer L
JournalExp Eye Res
PubMed ID4361169
Affinity cytochemistry: the localization of lectin and antibody receptors on erythrocytes via the avidin-biotin complex.
AuthorsBayer EA, Wilchek M, Skutelsky E
JournalFEBS Lett
PubMed ID976475
Flow cytometric analyses and sorting of neural cells for transplantation.
AuthorsNotter MF, Gash DM, Leary JF
JournalProg Brain Res
PubMed ID3247456
Staining of glycoproteins in polyacrylamide and agarose gels with fluorescent lectins.
AuthorsFurlan M, Perret BA, Beck EA
JournalAnal Biochem
PubMed ID91330
Fluorescent staining of living mouse neuromuscular junctions.
AuthorsKelly SS, Anis N, Robbins N
JournalPflugers Arch
PubMed ID3925434
A technique for staining living neuromuscular junctions is described and used for electrophysiological recording with both intra- and extra-cellular microelectrodes. This technique may be used on thicker muscles (eg. mouse soleus), has no effect on any of several physiological parameters tested, and should have several useful applications. ... More
The carbohydrates associated with the surfaces of hamster alveolar macrophages: a study using fluorescein-conjugated lectins.
AuthorsMeban C
JournalJ Anat
PubMed ID3935630
Alveolar macrophages were isolated from the lungs of hamsters by lavage and centrifugation. The harvesting procedure yielded about 5.2 X 10(6) cells per animal and the majority of these cells were viable as assessed by a Trypan blue exclusion test. Monolayers of macrophages were labelled with a series of FITC-conjugated ... More
A technique for fluorescence microscopy in semithin sections.
AuthorsOjeda JL, Ros MA, Icardo JM
JournalStain Technol
PubMed ID2699110
We describe here a procedure to improve contrast and resolution in fluorescence microscopy of sectioned tissues. Tissue fragments were fixed in ethanol-glacial acetic acid, embedded in diethylene glycol distearate, and semithin sectioned. This method maintains tissue antigenicity while preserving the structure of cells and tissues. The thinness of the sections ... More
Evaluation of a fluorescent lectin-based staining technique for some acidophilic mining bacteria.
AuthorsFife DJ, Bruhn DF, Miller KS, Stoner DL
JournalAppl Environ Microbiol
PubMed ID10788401
A fluorescence-labeled wheat germ agglutinin staining technique (R. K. Sizemore et al., Appl. Environ. Microbiol. 56:2245-2247, 1990) was modified and found to be effective for staining gram-positive, acidophilic mining bacteria. Bacteria identified by others as being gram positive through 16S rRNA sequence analyses, yet clustering near the divergence of that ... More
Conjugation of lectins with fluorochromes: an approach to histochemical double labeling of carbohydrate components.
AuthorsRoth J, Binder M, Gerhard UJ
JournalHistochemistry
PubMed ID80399
Methodical investigations on the coupling of lectins (Con A, LcL, WGA, RcA) to tetramethylrhodamine isothiocyan ate (TRITC) are reported. 20-microgram of TRITC per mg of lectin were found to be the optimal amount of TRITC for the conjugation. With this fluorochrome: protein ratio conjugates were produced which resulted in a ... More
Spatial distribution of cortical proteins in cells of epithelial sheets.
AuthorsOpas M, Kalnins VI
JournalCell Tissue Res
PubMed ID3919953
In the differentiated pigmented epithelial cells of the retina (RPE) of chick embryos cytoskeletal proteins are found in polygonal rings located in the cell cortex. Within the cortical rings of the RPE cells vinculin and spectrin occupy a characteristic position closest to the plasma membrane; actin is found farther away, ... More
Schistosoma mansoni: visualization with fluorescent lectins of secretions and surface carbohydrates of living cercariae.
AuthorsLinder E
JournalExp Parasitol
PubMed ID3996523
Attachment of Schistosoma mansoni cercariae was studied during their explorative movements along a glass surface using labeled lectins as markers. Fluorochrome-labeled lectins selectively labeled surface material produced at the cercarial attachment sites and part of the cercarial surface. The deposited secretions reacted with most of the lectins used but differences ... More
Flow cytometric analysis of lectin binding to human peripheral blood lymphocytes.
AuthorsBlackledge G, Vose B, Morris AJ, Crowther D, Gallagher JT
JournalBiochem J
PubMed ID6818949
The binding of fluorescein-conjugated lentil lectin and concanavalin A to the surface membrane of human peripheral blood lymphocytes was studied by flow cytometry. The lymphocytes bound 3-fold more lentil lectin molecules compared with concanavalin A molecules and lentil lectin binding approached saturation at a much lower concentration than did that ... More
Internalization of wheat germ agglutinin (WGA) by rat pancreatic cells in vivo and in vitro.
AuthorsWirth C, Schwuchow J, Jonas L
JournalActa Histochem
PubMed ID8739301
The uptake of the lectin wheat germ agglutinin (WGA) by the rat pancreas was studied after intraperitoneal injection of FITC-labelled WGA. Strong fluorescence was seen in the interstitial space of the pancreatic parenchyma and on the basolateral surface of acinar cells after one h. Two or four h after injection ... More
Lectin binding in human breast cancer: clinical and pathologic correlations with fluorescein-conjugated peanut, wheat germ and concanavalin A binding.
AuthorsDansey R, Murray J, Ninin D, Bezwoda WR
JournalOncology
PubMed ID3387034
Cell surface glycoconjugates of human breast cancer tissue were investigated using FITC peanut (PNA), wheat germ (WGA) and jackbean (concanavalin A; Con A) agglutinins. Although PNA and WGA binding patterns differed when normal and malignant breast tissues were compared, the specificity of this finding was poor and neither PNA nor ... More
Yeast surface display of trifunctional minicellulosomes for simultaneous saccharification and fermentation of cellulose to ethanol.
AuthorsWen F, Sun J, Zhao H,
JournalAppl Environ Microbiol
PubMed ID20023102
By combining cellulase production, cellulose hydrolysis, and sugar fermentation into a single step, consolidated bioprocessing (CBP) represents a promising technology for biofuel production. Here we report engineering of Saccharomyces cerevisiae strains displaying a series of uni-, bi-, and trifunctional minicellulosomes. These minicellulosomes consist of (i) a miniscaffoldin containing a cellulose-binding ... More
Differential binding of fluorescein-labeled lectins to mouse thymocytes: subsets revealed by flow microfluorometry.
AuthorsFowlkes BJ, Waxdal MJ, Sharrow SO, Thomas CA, Asofsky R, Mathieson BJ
JournalJ Immunol
PubMed ID6967084
Fluorescein-labeled lectins bound to mouse thymocytes were analyzed by flow microfluorometry. This technique has identified several lectins that bind differentially to thymocyte subsets. The most complex fluorescence distributions were obtained using lectins with nominal specificities for galactose or N-acetylglucosamine. Inhibition of binding by sugars confirmed that the fluoresceinated lectins were ... More
Subcellular compartmentalization of saccharide moieties in cultured normal and malignant cells.
AuthorsVirtanen I, Ekblom P, Laurila P
JournalJ Cell Biol
PubMed ID7372714
We studied subcellular localization of saccharide moieties in cultured normal and malignant cells fixed in paraformaldehyde and treated with a nonionic detergent, using lectins specific for various surgar residues as probes in fluorescence microscopy. In normal cells, concanavalin A and Lens culinaris agglutinin, specific for mannose-rich carbohydrate cores in glycoproteins, ... More
Lectin receptors on the plasma membrane of soybean cells. Binding and lateral diffusion of lectins.
AuthorsMetcalf TN, Wang JL, Schubert KR, Schindler M
JournalBiochemistry
PubMed ID6684479
Protoplasts prepared from suspension cultures of root cells of Glycine max (SB-1 cell line) bound soybean agglutinin (SBA), concanavalin A (Con A), and wheat germ agglutinin (WGA). Binding studies carried out with 125I-labeled SBA, Con A, and WGA showed that these interactions were saturable and specific. Fluorescence microscopy demonstrated uniform ... More
Role of membrane potential in the regulation of lectin-induced calcium uptake.
AuthorsGelfand EW, Cheung RK, Grinstein S
JournalJ Cell Physiol
PubMed ID6501431
Incubation of lymphocytes with mitogenic lectins triggers Ca2+ uptake. This increase in free cytoplasmic Ca2+ is postulated to be an important signal in the initiation of DNA synthesis. Transmembrane fluxes of monovalent ions and changes in membrane potential are also associated with lectin-induced activation of lymphocytes. We have examined the ... More
Reduced insulin endocytosis in serum-transformed fibroblasts demonstrated by flow cytometry.
AuthorsMurphy RF, Powers S, Cantor CR, Pollack R
JournalCytometry
PubMed ID6376000
Neoplastic transformation often results in the loss of growth control and concomitant changes in cell surface properties. The changes in endocytosis of a variety of probes after serum or anchorage transformation were measured for mouse fibroblasts by flow cytofluorometry. No major differences in dextran (fluid phase) or histone (nonspecific-adsorptive) endocytosis ... More
Rapid redistribution of Golgi proteins into the ER in cells treated with brefeldin A: evidence for membrane cycling from Golgi to ER.
In cells treated with brefeldin A (BFA), movement of newly synthesized membrane proteins from the endoplasmic reticulum (ER) to the Golgi apparatus was blocked. Surprisingly, the glycoproteins retained in the ER were rapidly processed by cis/medial Golgi enzymes but not by trans Golgi enzymes. An explanation for these observations was ... More
Study of surface carbohydrates on isolated Golgi subfractions by fluorescent-lectin binding and flow cytometry.
AuthorsGuasch RM, Guerri C, O'Connor JE
JournalCytometry
PubMed ID7743891
The Golgi complex is a functionally heterogeneous subcellular structure that plays a key role in the synthesis, maturation, and sorting of newly synthesized glycoproteins. Fluorescent lectins have been used extensively to analyze surface glycoproteins by flow cytometry in whole cells and more recently in isolated subcellular organelles, such as mitochondria ... More
Alterations in glomerular lectin binding sites of human kidney as detected by fluorescence microscopy.
AuthorsHolthöfer H, Virtanen I, Törnroth T, Miettinen A
JournalHistochem J
PubMed ID2415488
Different lectins were used to study frozen sections of kidney samples showing alterations in routine immunofluorescence studies. Arachis hypogaea agglutinin (peanut lectin, PNA), lacking binding sites in normal glomeruli, bound to the glomeruli in two of the five samples studied, giving a granular fluorescence pattern. Concomitantly with the appearance of ... More
Transfer of secretory proteins from the endoplasmic reticulum to the Golgi apparatus: discrimination between homologous and heterologous transfer in intact heterokaryons.
AuthorsValtersson C, Dutton AH, Singer SJ
JournalProc Natl Acad Sci U S A
PubMed ID2172977
To examine aspects of the transfer of secretory proteins from the endoplasmic reticulum to the Golgi apparatus in situ, heterokaryons were formed between Hep G2 human hepatoma cells and WI-38 human fibroblasts. The cells were appropriately treated with cycloheximide before fusion, which emptied them of their respective secretory proteins, serum ... More
Saccharides localized by fluorescent lectins on trophectoderm and endometrium prior to implantation in pigs, sheep and equids.
AuthorsWhyte A, Robson T
JournalPlacenta
PubMed ID6441928
Pre-implantation blastocysts together with the corresponding maternal endometrium were obtained from pigs, sheep and equids at known stages of pregnancy. Thin sections of fixed tissue were reacted with FITC-labelled conjugates of lectins specific for alpha-L-fucose, N-acetyl-D-glucosamine, D-galactose, N-acetyl-D-galactosamine and alpha-D-manno- and alpha-D-gluco-pyranosyl residues. The pre-implantation blastocysts of both pig and ... More
Characterization of rat colonic cell surface glycoconjugates by fluoresceinated lectins. I. Importance of fixation techniques.
AuthorsBrasitus TA, Goldfarb JP, Hsu KC, Tannenbaum M
JournalHistochemistry
PubMed ID6819259
Cryostat and paraffin embedded sections from cecum, proximal and distal colonic segments of male Sherman rats were examined by fluorescence microscopy after labeling with six fluorescein-conjugated lectins. These FITC-conjugated lectins were used as specific probes to define the labeling pattern of carbohydrate containing components of the lumenal and basolateral surfaces ... More
Fragmentation and dispersal of Golgi proteins and redistribution of glycoproteins and glycolipids processed through the Golgi apparatus after infection with herpes simplex virus 1.
In Vero monkey cells and HEp-2 human epidermoid carcinoma cells infected with herpes simplex virus 1 the proteins beta-COP, galactosyltransferase, and alpha-mannosidase II associated with the Golgi apparatus appear to be associated with numerous smaller structures dispersed throughout the cytoplasm. Concomitantly, the intracytoplasmic ligands of lectins normally associated wholly (Helix ... More
Wheat germ agglutinin-positive cells in a stem cell-enriched fraction of mouse bone marrow have potent natural suppressor activity.
AuthorsSugiura K, Inaba M, Ogata H, Yasumizu R, Inaba K, Good RA, Ikehara S
JournalProc Natl Acad Sci U S A
PubMed ID2968608
In the present study we have characterized natural suppressor (NS) cells, which nonspecifically suppress mitogen responses and mixed-lymphocyte reaction. The strongest NS activity was found in a fraction of relatively low-density cells (1.063 less than p less than 1.075) obtained by equilibrium density centrifugation. Further purification and characterization of these ... More
Lateral diffusion in nuclear membranes.
AuthorsSchindler M, Holland JF, Hogan M
JournalJ Cell Biol
PubMed ID3988794
Chemical modification of rat liver nuclei with citraconic anhydride selectively removed outer nuclear membrane. This conclusion was based on (a) transmission electron microscopy, (b) lipid analysis, (c) lamin B as an inner membrane-associated marker, and (d) the demonstration of phospholipid lateral mobility on outer membrane-depleted nuclei as a criteria for ... More
Rabies virus entry into endosomes in IMR-32 human neuroblastoma cells.
AuthorsLewis P, Fu Y, Lentz TL
JournalExp Neurol
PubMed ID9743568
Early events in rabies virus entry into cultured IMR-32 human neuroblastoma cells were investigated. After adsorption of rabies virus to the cell surface in the cold and warming to 37 degrees C in the presence of tracers for early endosomes, rabies virus and tracers were localized by immunofluorescence microscopy. After ... More
Lectin binding to rat spermatogenic cells: effects of different fixation methods and proteolytic enzyme treatment.
AuthorsMalmi R, Söderström KO
JournalHistochem J
PubMed ID3061982
The binding of a panel of eight different fluorescein-conjugated lectins to rat spermatogenic cells was investigated. Particular attention was paid to the effects of different fixation methods and proteolytic enzyme digestion on the staining pattern. Concanavalin A (Con A), wheatgerm agglutinin (WGA), succinylated WGA (s-WGA) and agglutinin from gorse (UEA ... More
Characterization of Yeast Yea4p, a uridine diphosphate-N-acetylglucosamine transporter localized in the endoplasmic reticulum and required for chitin synthesis.
AuthorsRoy SK, Chiba Y, Takeuchi M, Jigami Y
JournalJ Biol Chem
PubMed ID10788474
Chitin is an essential cell wall component, synthesis of which is regulated throughout the cell cycle in the yeast Saccharomyces cerevisiae. We cloned an S. cerevisiae gene, YEA4, whose product is homologous to the Kluyveromyces lactis uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter. An epitope-tagged Yea4p localized mainly in the 10,000 x g ... More
Rearrangement of Con A and WGA receptors on murine mastocytoma cell membranes: relationship to cell cycle.
AuthorsLustig S, Pluznik DH
JournalJ Cell Physiol
PubMed ID6403559
Incubation of interphase murine mastocytoma cells with fluorescein-labeled wheat germ agglutinin (FITC-WGA) or with rhodamine-labeled concanavalin A (Rh-Con A) shows binding of the lectins to all the cells in a ring form distribution. Treatment of the cells with trypsin before addition of FITC-WGA, or with colchicine before addition of Rh-Con ... More
Carbohydrate exposure on salmonella and E. coli bacteria after reaction with antibody IgG and secretory IgA (SIgA) assessed with fluorescent lectins.
AuthorsMagnusson KE, Edebo L
JournalImmunol Commun
PubMed ID6430789
The carbohydrate moieties exposed on enterobacteria before and after antibody binding have been tested with fluorescent lectins. Salmonella typhimurium 395 MS (S-type) and its Rd-mutant MR10 were coated with hyperimmune anti-MS and anti-MR 10 IgG, respectively. MR 10 bacteria and Escherichia coli O86 bacteria were coated with human colostral secretory ... More
Effects of calcium on vasopressin-mediated cyclic adenosine monophosphate formation in cultured rat inner medullary collecting tubule cells. Evidence for the role of intracellular calcium.
AuthorsTeitelbaum I, Berl T
JournalJ Clin Invest
PubMed ID3009546
We explored the effects of alterations in extracellular and intracellular calcium concentration on arginine vasopressin (AVP)-stimulated cAMP formation in cultured rat inner medullary collecting tubule cells. cAMP formation remains constant at extracellular calcium concentrations between 0.5 and 4.0 mM, which did not change intracellular calcium. Maneuvers that alter intracellular calcium ... More
Modulation of an NCAM-related adhesion molecule with long-term synaptic plasticity in Aplysia.
AuthorsMayford M, Barzilai A, Keller F, Schacher S, Kandel ER
JournalScience
PubMed ID1585176
A form of learning in the marine mollusk Aplysia, long-term sensitization of the gill- and siphon-withdrawal reflex, results in the formation of new synaptic connections between the presynaptic siphon sensory neurons and their target cells. These structural changes can be mimicked, when the cells are maintained in culture, by application ... More
Differential binding of lectins to lymphopoietic and myelopoietic cells in murine marrow as revealed by flow cytometry.
AuthorsReimann J, Ehman D, Miller RG
JournalCytometry
PubMed ID6714027
Murine marrow cells were labeled with a panel of fluorescein-conjugated lectins and analyzed by flow cytometry. All lectins tested [concanavalin A (Con A), phytohemagglutinin (PHA), wheat germ agglutinin (WGA), pokeweed mitogen (PWM), soybean agglutinin (SBA), peanut agglutinin (PNA), lentil lectin (LL)], stained a fraction of marrow cells; the fraction was, ... More
Ultrastructural localization of lectin receptors on the surface of the rat retinal pigment epithelium. Decreased sensitivity of the avidin-biotin method due to cell surface charge.
AuthorsPino RM
JournalJ Histochem Cytochem
PubMed ID6747275
Monosaccharides on the apical processes of the retinal pigment epithelium were examined using lectin-affinity cytochemical methods. Lectin receptor sugars were localized with lectin-horseradish peroxidase (HRP) and lectin-ferritin conjugates as well as with biotinylated lectins, avidin, and biotinylated HRP. In contrast, only wheat germ agglutinin (WGA) receptors were identified with biotinylated ... More
Properties of succinylated wheat-germ agglutinin.
AuthorsMonsigny M, Sene C, Obrenovitch A, Roche AC, Delmotte F, Boschetti E
JournalEur J Biochem
PubMed ID467446
The physicochemical and binding properties of succinylated wheat germ agglutinin are described in comparison with these of unmodified wheat germ agglutinin. Succinylated wheat germ agglutinin is an acidic protein with a pI of 4.0 +/- 0.2 while the native lectin is basic, pI of 8.5. The solubility of succinylated wheat ... More
Kinetoplastid flagellates: surface-reactive carbohydrates detected by fluorescein-conjugated lectins.
AuthorsGhosh DK, Ghosh AK, Ghosh KN, De A, Bhattacharya A
JournalJ Parasitol
PubMed ID2299519
Membrane-associated carbohydrate residues of 3 isolates of Leishmania derived from etiological agents of visceral leishmaniasis (VL), postkala-azar dermal leishmaniasis (PKDL), and cutaneous leishmaniasis (CL), as well as 2 other nonpathogenic insect gut kinetoplastid flagellates, Bodo sp. and Herpetomonas sp., were characterized with the aid of 8 fluorescein-conjugated lectins. Four lectins, ... More
Nucleocytoplasmic shuttling: a novel in vivo property of antisense phosphorothioate oligodeoxynucleotides.
Phosphorothioate oligodeoxynucleotides (P=S ODNs) are frequently used as antisense agents to specifically interfere with the expression of cellular target genes. However, the cell biological properties of P=S ODNs are poorly understood. Here we show that P=S ODNs were able to continuously shuttle between the nucleus and the cytoplasm and that ... More
Rapid lateral diffusion of lectin-labelled glycoconjugates in the human colonic adenocarcinoma cell line HT29. Comparison with the synthetic lipid analogue diI-C14.
AuthorsMagnusson KE, Johansson B, Sundqvist T
JournalEur Biophys J
PubMed ID3484360
The lateral diffusion of lectin-labelled glycoconjugates was studied in the human colon carcinoma cell line HT29 using fluorescence photobleaching techniques. HT29 cells were grown in either Dulbecco's modified Eagle's medium with glucose (25 mM; DMEM-Glu) or with galactose (25 mM; DMEM-Gal). Cell cultivation in the DMEM-Gal medium was assumed to ... More
Analysis of the secretions of the subcommissural organs of several vertebrate species by use of fluorescent lectins.
AuthorsMeiniel R, Meiniel A
JournalCell Tissue Res
PubMed ID3919951
The glycoprotein secretions of the subcommissural organ were analyzed with the use of nine fluorescent lectins, specific to different sugar moieties. After exposure to Concanavalin A a bright fluorescence was observed in the ependymal cells of the subcommissural organs of all vertebrates studied (Lampetra planeri, Ameiurus nebulosus, Bufo bufo, Lacerta ... More
Coupling of biotin-(poly(ethylene glycol))amine to poly(D,L-lactide-co-glycolide) nanoparticles for versatile surface modification.
AuthorsWeiss B, Schneider M, Muys L, Taetz S, Neumann D, Schaefer UF, Lehr CM
JournalBioconjug Chem
PubMed ID17590034
Generally, polymeric nanoparticles (NP) for drug targeting are designed to entrap the drug moiety in the core and to present the targeting moiety on the surface. However, in most cases, common preparation techniques of polymeric NP need to be specifically arranged for each compound to be entrapped or attached. In ... More
Human munc13 is a diacylglycerol receptor that induces apoptosis and may contribute to renal cell injury in hyperglycemia.
AuthorsSong Y, Ailenberg M, Silverman M
JournalMol Biol Cell
PubMed ID10233166
We have previously shown that human munc13 (hmunc13) is up-regulated by hyperglycemia under in vitro conditions in human mesangial cell cultures. The purpose of the present study was to determine the cellular function of hmunc13. To do this, we have investigated the subcellular localization of hmunc13 in a transiently transfected ... More
Fluorescence energy transfer studies on lima bean lectin. Distance between the subunit hydrophobic binding site and the thiol group essential for carbohydrate binding.
AuthorsKella NK, Roberts DD, Shafer JA, Goldstein IJ
JournalJ Biol Chem
PubMed ID6715322
Measurements of the efficiency of singlet-singlet energy transfer were used to determine the distance between the hydrophobic binding site and the thiol group required for carbohydrate-binding activity of lima bean lectin. 1-Anilino-8-naphthalenesulfonate, bound to the hydrophobic binding site by noncovalent interactions, was used as the donor. Two different nonfluorescent probes ... More
The use of fluorochromes in the cytochemical characterization of some phytoflagellates.
AuthorsKlut ME, Bisalputra T, Antia NJ
JournalHistochem J
PubMed ID2453490
Sixteen fluorochromes were tested for the cytochemical characterization of two dinoflagellates (Amphidinuim carterae, Prorocentrum micans) and one chlorophycean flagellate (Dunaliella tertiolecta). Depending on the fluorochrome used, various cellular components (including the plasma membrane, thecal plates, pusule, trichocysts, nucleus, lipid bodies and vacuoles) were revealed. The different colours obtained from single ... More
Two-photon microscopy of vital murine elastic and muscular arteries. Combined structural and functional imaging with subcellular resolution.
AuthorsMegens RT, Reitsma S, Schiffers PH, Hilgers RH, De Mey JG, Slaaf DW, oude Egbrink MG, van Zandvoort MA
JournalJ Vasc Res
PubMed ID17192719
Understanding vascular pathologies requires insight in the structure and function, and, hence, an imaging technique combining subcellular resolution, large penetration depth, and optical sectioning. We evaluated the applicability of two-photon laser-scanning microscopy (TPLSM) in large elastic and small muscular arteries under physiological conditions. Elastic (carotid) and muscular (uterine, mesenteric) arteries ... More