Please note that at certain times of the year Bis-Tris protein gel boxes may arrive inside insulated coolers containing ambient temperature gel packs.

NuPAGE™ 4-12% Bis-Tris Midi Protein Gels, 20-well

Catalog number:  WG1402BOX

 Related applications: Protein Gel Electrophoresis

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


Invitrogen NuPAGE Bis-Tris Midi Gels are precast polyacrylamide gels designed to give optimal separation of a wide range of proteins under denaturing conditions. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for the preparation of proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. Also, use NuPAGE gels for optimal results during day-to-day use. NuPAGE Bis-Tris gels come in two sizes and multiple well formats.

Features of NuPAGE Bis-Tris gels:
• Faster run times—get separation of your proteins in as little as 35 minutes
• Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature
• Better protein integrity—optimized sample preparation process preserves your proteins
• Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins

Learn more about all of our available NuPAGE Bis-Tris gels >
View migration charts ›

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris midi gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. The gels can be run using NuPAGE MES or NuPAGE MOPS SDS running buffer to obtain different separation ranges.

Choose the right NuPAGE Bis-Tris gel for the size of your samples
The gels come in two sizes: mini (10 cm x 10 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

Fast and convenient to use
NuPAGE Bis-Tris gels can be run in as little as 35 minutes when using the NuPAGE MES running buffer. NuPAGE Bis-Tris gels have a shelf life of at least 12 months at 4–25°C. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. The gels also provide sharper bands, higher resolution, longer shelf-life, and higher accuracy of results.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent with your samples. Use NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and allow maximum band sharpness. We recommend using Novex Sharp Pre-stained Protein Standard; this protein standard contains our widest molecular weight range with 12 protein bands ranging from 3.5–260 kDa. Get fast and sensitive staining of your protein gels with our SimplyBlue SafeStain.

We also provide NuPAGE Tris-acetate gels for separating larger proteins. For classic Laemmli-based Tris-Glycine electrophoresis, we offer Novex Tris-glycine gels.

NuPAGE Bis-Tris Midi gels can be used with the XCell SureLock Midi Cell gel apparatuses or conveniently with the Bio-Rad Criterion Cell using our adapters.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer buffer. Rapid semi-dry transfer can be done using the Pierce Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device. Alternatively, traditional wet transfer can be performed using the Bio-Rad Criterion Cell.

Related links
Overview of 1D Protein Electrophoresis
Comparison of NuPAGE Tris-Bis vs. traditional Tris-glycine gels

For Research Use Only. Not for use in diagnostic procedures.


Gel Percentage: 4-12 %
Wells: 20 wells
Gel Type: Bis-Tris Gel
Separation Range: 15 to 260 kDa (MOPS buffer), 3.5 to 160 kDa (MES buffer)
Gel Size: Midi (8 cm x 13 cm)
Well Format: 1D Well
Thickness: 1.0 mm
Product Line: Novex™, NuPAGE®
Adapters: No Adapters
For Use With (Equipment): XCell4 SureLock™ Midi
Mode of Separation: Molecular Weight
Product Size: 10 gels (1 box)
Quantity: 10 gels
Sample Loading Volume: up to 25 µL
System: NuPAGE® Novex®
Shipping Condition: Room Temperature

Contents & storage

One box contains 10 gels. Store at 4–25°C. Do not freeze. Shelf life is 12 months.


Manuals & protocols

Recommended products