X-Rhod-1, AM, cell permeant - Special Packaging
X-Rhod-1, AM, cell permeant - Special Packaging
Citations & References (40)
Invitrogen™

X-Rhod-1, AM, cell permeant - Special Packaging

Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+. They have uses in many calciumRead more
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Catalog NumberQuantity
X1421010 x 50 μg
Catalog number X14210
Price (MXN)
-
Quantity:
10 x 50 μg
Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+. They have uses in many calcium signaling investigations, including measuring Ca2+ in cells and tissues that have high levels of autofluorescence and also for detecting Ca2+ release generated by photoreceptors and photoactivatable chelators. Cells may be loaded with the AM ester forms of these calcium indicators by adding the dissolved indicator directly to dishes containing cultured cells. The fluorescence signal from these cells is generally measured using fluorescence microscopy.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›

Calcium Indicator (AM Ester) Specifications:

  • Label (Ex/Em of Ca2+–bound form): X-rhod-1 (580/602 nm)
  • Fluorescence intensity increase upon binding Ca2+: >100 fold
  • Kd for Ca2+ in the absence of Mg2+, in buffer: ∼700 nM
  • Exhibit fluorescence increase upon binding Ca2+ with little shift in wavelength

    Using TPEN to Control Heavy Metal Cations

    In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn2+, Zn2+, Pb2+) with substantially higher affinity than Ca2+. Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelator TPEN.

    More Choices for Fluorescent Calcium Indicators

    We offer a large selection of Molecular Probes™ calcium indicators for use in various experimental scenarios, for example dextran versions for reduced leakage and compartmentalization and BAPTA conjugates for detecting high-amplitude calcium transients. For more information, review Fluorescent Ca2+ Indicators Excited with Visible Light—Section 19.3 in the Molecular Probes™ Handbook.

    For UV-excitable Ca2+ indicators, protein-based Ca2+ indicators, conjugates of Ca2+ indicators, and for fluorescence-based indicators of other metal ions (i.e., Mg2+, Zn2+) review Indicators for Ca2+, Mg2+, Zn2+ and Other Metal Ions—Chapter 19 in the Molecular Probes™ Handbook.

    For Research Use Only. Not for human or animal therapeutic or diagnostic use.

    • For Research Use Only. Not for use in diagnostic procedures.
    Specifications
    Detection MethodFluorescence
    Dye TypeFluorescent Dye-Based
    Quantity10 x 50 μg
    Shipping ConditionRoom Temperature
    For Use With (Application)Cell Viability and Proliferation
    For Use With (Equipment)Fluorescence Microscope
    Product TypeDye
    Unit Size10 x 50 µg
    Contents & Storage
    Store in freezer -5°C to -30°C and protect from light.

    Citations & References (40)

    Citations & References
    Abstract
    Mitochondrial modulation of Ca2+ -induced Ca2+ -release in rat sensory neurons.
    Authors:Jackson JG, Thayer SA
    Journal:J Neurophysiol
    PubMed ID:16760347
    'Ca2+ -induced Ca2+ -release (CICR) from ryanodine-sensitive Ca2+ stores provides a mechanism to amplify and propagate a transient increase in intracellular calcium concentration ([Ca2+]i). A subset of rat dorsal root ganglion neurons in culture exhibited regenerative CICR when sensitized by caffeine. [Ca2+]i oscillated in the maintained presence of 5 mM ... More
    Selective, high-resolution fluorescence imaging of mitochondrial Ca2+ concentration.
    Authors:Gerencsér AA AA, Adam-Vizi V
    Journal:Cell Calcium
    PubMed ID:11733937
    'We have developed a digital image processing technique based on highpass filtering of microfluorimetric images for selective transmission of fine image details corresponding to mitochondria. This technique enabled the detection of the mitochondrial calcium signals with high selectivity, simultaneously with the cytosolic calcium signal. The validity of this technique was ... More
    Use of fluorescent Ca2+ dyes with green fluorescent protein and its variants: problems and solutions.
    Authors:Bolsover S, Ibrahim O, O'luanaigh N, Williams H, Cockcroft S
    Journal:Biochem J
    PubMed ID:11368760
    'We have studied the degree to which fluorescent Ca(2+) indicator dyes, and green fluorescent protein and its variants, can be used together. We find that the most commonly used fluorescent protein, enhanced green fluorescent protein (EGFP), seriously contaminates fura 2 signals. We suggest two alternative combinations for which there is ... More
    CXCR4 regulates growth of both primary and metastatic breast cancer.
    Authors:Smith MC, Luker KE, Garbow JR, Prior JL, Jackson E, Piwnica-Worms D, Luker GD
    Journal:Cancer Res
    PubMed ID:15574767
    'The chemokine receptor CXCR4 and its cognate ligand CXCL12 recently have been proposed to regulate the directional trafficking and invasion of breast cancer cells to sites of metastases. However, effects of CXCR4 on the growth of primary breast cancer tumors and established metastases and survival have not been determined. We ... More
    A novel method for assessing effects of hydrostatic fluid pressure on intracellular calcium: a study with bovine articular chondrocytes.
    Authors:Mizuno S
    Journal:Am J Physiol Cell Physiol
    PubMed ID:15643052
    'Chondrocytes in articular cartilage are exposed to hydrostatic pressure and distortional stress during weight bearing and joint loading. Because these stresses occur simultaneously in articular cartilage, the mechanism of mechanosignal transduction due to hydrostatic pressure alone in chondrocytes is not clear. In this study, we attempted to characterize the change ... More
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