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View additional product information for Monomeric Cyanine Nucleic Acid Stains - FAQs (Y3603, P3581, T3605, Y3607, T3602)
3 product FAQs found
For fluorescence microscopy, the working concentration of Monomeric Cyanine Nucleic Acid Stains (Cat. Nos. T3605, P3581, T3602, Y3603, Y3607) is typically 1 - 10 µM. You may need to titrate the dye solution to optimize the working concentration for your sample type and application.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
For flow cytometry, the working concentration of Monomeric Cyanine Nucleic Acid Stains (Cat. Nos. T3605, P3581, T3602, Y3603, Y3607) is typically 25 nM - 1 µM. You may need to titrate the dye solution to optimize the working concentration for your sample type and application.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
See Table 1 in this article (http://www.thermofisher.com/content/dam/LifeTech/migration/en/filelibrary/support/bioprobes/bioprobes-65.par.61751.file.dat/bioprobes-65-cellevent.pdf) where a Jurkat model system was induced with 10 µM camptothecin for time periods of 0 to 4 hours. It is important to note that these results were studied using a single cell type and induction system; results may differ for other experimental systems. Increased membrane permeability in apoptotic cells can be discriminated from dead cells using YO-PRO-1 dye in combination with propidium iodide or the SYTOX dead cell indicators. Other mid-apoptotic events are increased ROS production (detected with CellROX reagents, H2DCFDA), changes in cellular pH (BCECF, SNARF-1) and calcium release (Fluo-4, Fura-2, Indo-1). No single parameter defines apoptosis under any condition, so it is best to employ a multi-parametric approach when studying apoptosis.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.