Effect of the substitution of muscle actin-specific subdomain 1 and 2 residues in yeast actin on actin function.
AuthorsMcKane M, Wen KK, Meyer A, Rubenstein PA
JournalJ Biol Chem
PubMed ID16882670
'Muscle and yeast actins display distinct behavioral characteristics. To better understand the allosteric interactions that regulate actin function, we created a muscle/yeast hybrid actin containing a muscle-specific outer domain (subdomains 1 and 2) and a yeast inner domain (subdomains 3 and 4). Actin with muscle subdomain 1 and the two ... More
Structure, function, and motility of vacuoles in filamentous fungi
AuthorsCole L, Orlovich DA, Ashford AE
JournalFungal Genet Biol
PubMed ID9742195
'Current information on the structure and function of motile tubular vacuoles in Pisolithus tinctorius and other fungi is reviewed. The use of fluorochromes to label the vacuole lumen is evaluated and observations on the structure and motility of vacuoles in P. tinctorius are differentiated from possible artifacts. The styryl dyes ... More
Interdependent assembly of specific regulatory lipids and membrane fusion proteins into the vertex ring domain of docked vacuoles.
AuthorsFratti RA, Jun Y, Merz AJ, Margolis N, Wickner W
JournalJ Cell Biol
PubMed ID15611334
'Membrane microdomains are assembled by lipid partitioning (e.g., rafts) or by protein-protein interactions (e.g., coated vesicles). During docking, yeast vacuoles assemble "vertex" ring-shaped microdomains around the periphery of their apposed membranes. Vertices are selectively enriched in the Rab GTPase Ypt7p, the homotypic fusion and vacuole protein sorting complex (HOPS)-VpsC Rab ... More
Physiological regulation of yeast cell death in multicellular colonies is triggered by ammonia.
AuthorsVáchová L, Palková Z
JournalJ Cell Biol
PubMed ID15939758
The existence of programmed cell death (PCD) in yeast and its significance to simple unicellular organisms is still questioned. However, such doubts usually do not reflect the fact that microorganisms in nature exist predominantly within structured, multicellular communities capable of differentiation, in which a profit of individual cells is subordinated ... More
Remodeling of organelle-bound actin is required for yeast vacuole fusion.
AuthorsEitzen G, Wang L, Thorngren N, Wickner W
JournalJ Cell Biol
PubMed ID12177043
Actin participates in several intracellular trafficking pathways. We now find that actin, bound to the surface of purified yeast vacuoles in the absence of cytosol or cytoskeleton, regulates the last compartment mixing stage of homotypic vacuole fusion. The Cdc42p GTPase is known to be required for vacuole fusion. We now ... More
Ribosomal Protein uL11 as a Regulator of Metabolic Circuits Related to Aging and Cell Cycle.
Authors
JournalCells
PubMed ID32708309
Proximity proteomics in a marine diatom reveals a putative cell surface-to-chloroplast iron trafficking pathway.
Authors
JournalElife
PubMed ID33591270
A GDSL Esterase/Lipase Catalyzes the Esterification of Lutein in Bread Wheat.
Authors
JournalPlant Cell
PubMed ID33590251
A GDSL Esterase/Lipase Catalyzes the Esterification of Lutein in Bread Wheat.
Authors
JournalPlant Cell
PubMed ID31575724
14-Helical β-Peptides Elicit Toxicity against C. albicans by Forming Pores in the Cell Membrane and Subsequently Disrupting Intracellular Organelles.