Cocaine-induced dendritic spine formation in D1 and D2 dopamine receptor-containing medium spiny neurons in nucleus accumbens.
AuthorsLee KW, Kim Y, Kim AM, Helmin K, Nairn AC, Greengard P
JournalProc Natl Acad Sci U S A
PubMed ID16492766
'Psychostimulant-induced alteration of dendritic spines on dopaminoceptive neurons in nucleus accumbens (NAcc) has been hypothesized as an adaptive neuronal response that is linked to long-lasting addictive behaviors. NAcc is largely composed of two distinct subpopulations of medium-sized spiny neurons expressing high levels of either dopamine D1 or D2 receptors. In ... More
The second extracellular loop of CCR5 contains the dominant epitopes for highly potent anti-human immunodeficiency virus monoclonal antibodies.
AuthorsZhang J, Rao E, Dioszegi M, Kondru R, DeRosier A, Chan E, Schwoerer S, Cammack N, Brandt M, Sankuratri S, Ji C
JournalAntimicrob Agents Chemother
PubMed ID17242138
'Six mouse anti-human CCR5 monoclonal antibodies (mAbs) that showed potent antiviral activities were identified from over 26,000 mouse hybridomas. The epitopes for these mAbs were determined by using various CCR5 mutants, including CCR5/CCR2B chimeras. One mAb, ROAb13, was found to bind to a linear epitope in the N terminus of ... More
Comparison of methods for determining zeta-chain associated protein - 70 (ZAP-70) expression in patients with B-cell chronic lymphocytic leukemia (B-CLL).
AuthorsBojarska-Junak A, Giannopoulos K, Kowal M, Dmoszynska A, Rolinski J,
JournalCytometry B Clin Cytom
PubMed ID16906577
BACKGROUND: Zeta-chain associated protein of 70kDa (ZAP-70) is the most promising surrogate marker for the immunoglobulin heavy chain variable region (IgV(H)) mutation status in B-cell chronic lymphocytic leukemia (B-CLL). Crespo et al. proposed the detection of ZAP-70 by flow cytometry. Recently several novel monoclonal antibodies appeared on market. METHODS: We ... More
A screen for endocytic motifs.
AuthorsKozik P, Francis RW, Seaman MN, Robinson MS,
JournalTraffic
PubMed ID20214754
Sorting signals for cargo selection into coated vesicles are usually in the form of short linear motifs. Three motifs for clathrin-mediated endocytosis have been identified: YXXPhi, [D/E]XXXL[L/I] and FXNPXY. To search for new endocytic motifs, we made a library of CD8 chimeras with random sequences in their cytoplasmic tails, and ... More
Hereditary parkinsonism with dementia is caused by mutations in ATP13A2, encoding a lysosomal type 5 P-type ATPase.
AuthorsRamirez A, Heimbach A, Gründemann J, Stiller B, Hampshire D, Cid LP, Goebel I, Mubaidin AF, Wriekat AL, Roeper J, Al-Din A, Hillmer AM, Karsak M, Liss B, Woods CG, Behrens MI, Kubisch C,
JournalNat Genet
PubMed ID16964263
Neurodegenerative disorders such as Parkinson and Alzheimer disease cause motor and cognitive dysfunction and belong to a heterogeneous group of common and disabling disorders. Although the complex molecular pathophysiology of neurodegeneration is largely unknown, major advances have been achieved by elucidating the genetic defects underlying mendelian forms of these diseases. ... More
Bcl-2 on the endoplasmic reticulum regulates Bax activity by binding to BH3-only proteins.
AuthorsThomenius MJ, Wang NS, Reineks EZ, Wang Z, Distelhorst CW
JournalJ Biol Chem
PubMed ID12477729
Bcl-2 family members have been shown to be key mediators of apoptosis as either pro- or anti-apoptotic factors. It is thought that both classes of Bcl-2 family members act at the level of the mitochondria to regulate apoptosis, although the founding anti-apoptotic family member, Bcl-2 is localized to the endoplasmic ... More
Binding and transfer of human immunodeficiency virus by DC-SIGN+ cells in human rectal mucosa.
AuthorsGurney KB, Elliott J, Nassanian H, Song C, Soilleux E, McGowan I, Anton PA, Lee B
JournalJ Virol
PubMed ID15827191
The role of DC-SIGN on human rectal mucosal dendritic cells is unknown. Using highly purified human rectal mucosal DC-SIGN+ cells and an ultrasensitive real-time reverse transcription-PCR assay to quantify virus binding, we found that HLA-DR+/DC-SIGN+ cells can bind and transfer more virus than the HLA-DR+/DC-SIGN- cells. Greater than 90% of ... More
Self-renewing and differentiating properties of cortical neural stem cells are selectively regulated by basic fibroblast growth factor (FGF) signaling via specific FGF receptors.
AuthorsMaric D, Fiorio Pla A, Chang YH, Barker JL
JournalJ Neurosci
PubMed ID17314281
Developmental processes mediating the initiation of lineage commitment from self-renewing neural stem cells (NSCs) remain mostly unclear because of the persisting ambiguity in identifying true NSCs from proliferative lineage-restricted progenitors (LRPs), which are directly or indirectly derived from NSCs. Our multilineage immunohistochemical analyses of early embryonic rat telencephalon at the ... More
Erythropoietin Receptor-Mediated Molecular Crosstalk Promotes T Cell Immunoregulation and Transplant Survival.
AuthorsPurroy C, Fairchild RL, Tanaka T, Baldwin WM, Manrique J, Madsen JC, Colvin RB, Alessandrini A, Blazar BR, Fribourg M, Donadei C, Maggiore U, Heeger PS, Cravedi P
JournalJ Am Soc Nephrol
PubMed ID28302753
'Although spontaneous kidney transplant acceptance/tolerance occurs in mice and occasionally in humans, mechanisms remain unclear. Herein we test the hypothesis that EPO, a hormone predominantly produced by the adult kidney, has immunomodulating properties that are required for spontaneous kidney graft acceptance.' ... More
Arf6, JIP3, and dynein shape and mediate macropinocytosis.
AuthorsWilliamson CD, Donaldson JG
JournalMol Biol Cell
PubMed ID30969891
'Macropinocytosis is an actin-driven form of clathrin-independent endocytosis that generates an enlarged structure, the macropinosome. Although many studies focus on signaling molecules and phosphoinositides involved in initiating macropinocytosis, the commitment to forming a macropinosome and the handling of that membrane have not been studied in detail. Here we show in ... More
Fibered Confocal Fluorescence Microscopy for the Noninvasive Imaging of Langerhans Cells in Macaques.
AuthorsTodorova B, Salabert N, Tricot S, Boisgard R, Rathaux M, Le Grand R, Chapon C
JournalContrast Media Mol Imaging
PubMed ID29097915
'We developed a new approach to visualize skin Langerhans cells by in vivo fluorescence imaging in nonhuman primates.'
Jagged-1 Signaling in the Bone Marrow Microenvironment Promotes Endothelial Progenitor Cell Expansion and Commitment of CD133+ Human Cord Blood Cells for Postnatal Vasculogenesis.
AuthorsIshige-Wada M, Kwon SM, Eguchi M, Hozumi K, Iwaguro H, Matsumoto T, Fukuda N, Mugishima H, Masuda H, Asahara T
JournalPLoS One
PubMed ID27846321
Notch signaling is involved in cell fate decisions during murine vascular development and hematopoiesis in the microenvironment of bone marrow. To investigate the close relationship between hematopoietic stem cells and human endothelial progenitor cells (EPCs) in the bone marrow niche, we examined the effects of Notch signals [Jagged-1 and Delta-like ... More
Expression of Programmed Death-Ligand 1 by Human Colonic CD90
AuthorsBeswick EJ, Grim C, Singh A, Aguirre JE, Tafoya M, Qiu S, Rogler G, McKee R, Samedi V, Ma TY, Reyes VE, Powell DW, Pinchuk IV
JournalFront Immunol
PubMed ID29910803
The role of programmed cell death protein 1 (PD-1) and its ligands in the dysregulation of T helper immune responses observed in the inflammatory bowel disease (IBD) is unclear. Recently, a novel concept emerged that CD90 ... More
Different contributions of nonmuscle myosin IIA and IIB to the organization of stress fiber subtypes in fibroblasts.
AuthorsKuragano M, Uyeda TQP, Kamijo K, Murakami Y, Takahashi M
JournalMol Biol Cell
PubMed ID29467250
We demonstrated that myosin IIA and IIB are essential for the formation of transverse arcs and ventral stress fibers, respectively. Furthermore, we illustrated the roles of both isoforms in lamellar flattening and also raised the possibility that actin filaments in ventral stress fibers are in a stretched conformation. ... More
Epithelial CD47 is critical for mucosal repair in the murine intestine in vivo.
AuthorsReed M, Luissint AC, Azcutia V, Fan S, O'Leary MN, Quiros M, Brazil J, Nusrat A, Parkos CA
JournalNat Commun
PubMed ID31676794
CD47 is a ubiquitously expressed transmembrane glycoprotein that regulates inflammatory responses and tissue repair. Here, we show that normal mice treated with anti-CD47 antibodies, and Cd47-null mice have impaired intestinal mucosal wound healing. Furthermore, intestinal epithelial cell (IEC)-specific loss of CD47 does not induce spontaneous immune-mediated intestinal barrier disruption but ... More