Invitrogen™

Zenon™ Human IgG Labeling Kit

Catalog number: Z25402
Invitrogen™

Zenon™ Human IgG Labeling Kit

Catalog number: Z25402
Take advantage of a fast, versatile, and reliable method for producing antibody (IgG) conjugates for immunocytochemistry (ICC), immunohistochemistry (IHC), flow cytometry, and cell imaging with the Invitrogen Zenon™ Human IgG Labeling Kits. Simplify your laboratory applications and reduce antibody cross-reactivity while also achieving efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions.
 
Catalog Number
Z25402
also known as Z-25402
Unit Size
1 kit
Quantity
50 Reactions
Excitation/Emission
495/519
Label or Dye
Alexa Fluor™ 488
Price (USD)
457.00
Your Price:
Availability
***
Full specifications
Quantity
Kit ContentsContains 1 vial of Zenon™ Alexa Fluor™ 488 Human IgG labeling reagent (250 µL), and 1 vial of Zenon™ blocking reagent (rabbit IgG, 250 µL)
Quantity50 Reactions
Storage RequirementsStore in refrigerator (2–8°C) and protect from light
Excitation/Emission495/519
Labeling Scale<1 to 20 μg
Product LineZenon™
Label or DyeAlexa Fluor™ 488
Label TypeAlexa Fluor Dyes
ColorGreen
Labeling TargetHuman
Unit Size1 kit
Catalog Number
Z25407
also known as Z-25407
Unit Size
1 kit
Quantity
50 Reactions
Excitation/Emission
590/617
Label or Dye
Alexa Fluor™ 594
Price (USD)
449.00
Your Price:
Availability
***
Full specifications
Quantity
Kit ContentsContains 1 vial of Zenon™ Alexa Fluor™ 594 Human IgG labeling reagent (250 µL), and 1 vial of Zenon™ blocking reagent (rabbit IgG, 250 µL)
Quantity50 Reactions
Storage RequirementsStore in refrigerator (2–8°C) and protect from light
Excitation/Emission590/617
Labeling Scale<1 to 20 μg
Product LineZenon™
Label or DyeAlexa Fluor™ 594
Label TypeAlexa Fluor Dyes
ColorRed
Labeling TargetHuman
Unit Size1 kit
Catalog Number
Z25408
also known as Z-25408
Unit Size
1 kit
Quantity
50 Reactions
Excitation/Emission
650/665
Label or Dye
Alexa Fluor™ 647
Price (USD)
453.00
Your Price:
Availability
***
Full specifications
Quantity
Kit ContentsContains 1 vial of Zenon™ Alexa Fluor™ 647 Human IgG labeling reagent (250 µL), and 1 vial of Zenon™ blocking reagent (rabbit IgG, 250 µL)
Quantity50 Reactions
Storage RequirementsStore in refrigerator (2–8°C) and protect from light
Excitation/Emission650/665
Labeling Scale<1 to 20 μg
Product LineZenon™
Label or DyeAlexa Fluor™ 647
Label TypeAlexa Fluor Dyes
ColorFar-Red
Labeling TargetHuman
Unit Size1 kit
Catalog Number
Z25451
also known as Z-25451
Unit Size
1 kit
Quantity
50 Reactions
Excitation/Emission
650/660
Label or Dye
APC (Allophycocyanin)
Price (USD)
403.00
Your Price:
Availability
***
Full specifications
Quantity
Kit ContentsContains 1 vial of Zenon™ APC Human IgG labeling reagent (125 µL), and 1 vial of Zenon™ blocking reagent (rabbit IgG, 125 µL)
Quantity50 Reactions
Storage RequirementsStore in refrigerator (2–8°C) and protect from light
Excitation/Emission650/660
Labeling Scale<1 to 20 μg
Product LineZenon™
Label or DyeAPC (Allophycocyanin)
Label TypePE & APC
ColorRed
Labeling TargetHuman
Unit Size1 kit
Catalog Number
Z25452
also known as Z-25452
Unit Size
1 kit
Quantity
50 Reactions
Excitation/Emission
Label or Dye
Biotin-XX
Price (USD)
437.00
Your Price:
Availability
***
Full specifications
Quantity
Kit ContentsContains 1 vial of Zenon™ Biotin-XX Human IgG labeling reagent (250 µL), and 1 vial of Zenon™ blocking reagent (rabbit IgG, 250 µL)
Quantity50 Reactions
Storage RequirementsStore in refrigerator (2–8°C) and protect from light
Labeling Scale<1 to 20 μg
Product LineZenon™
Label or DyeBiotin-XX
Label TypeBiotin & Other Haptens
ColorColorless
Labeling TargetHuman
Unit Size1 kit
Catalog Number
Z25455
also known as Z-25455
Unit Size
1 kit
Quantity
25 Reactions
Excitation/Emission
496/578
Label or Dye
R-PE (R-Phycoerythrin)
Price (USD)
403.00
Your Price:
Availability
***
Full specifications
Quantity
Kit ContentsContains 1 vial of Zenon™ R-PE Human IgG labeling reagent (125 µL), and 1 vial of Zenon™ blocking reagent (rabbit IgG, 125 µL)
Quantity25 Reactions
Storage RequirementsStore in refrigerator (2–8°C) and protect from light
Excitation/Emission496/578
Labeling Scale<1 to 20 μg
Product LineZenon™
Label or DyeR-PE (R-Phycoerythrin)
Label TypePE & APC
ColorRed-Orange
Labeling TargetAntibodies (IgG)
Unit Size1 kit
Showing 6 of 6
Catalog NumberSpecificationsUnit SizeQuantityExcitation/EmissionLabel or DyePrice (USD)AvailabilityQuantity
Z25402
also known as Z-25402
Full specifications
1 kit50 Reactions495/519Alexa Fluor™ 488
457.00
Your Price:
***
Kit ContentsContains 1 vial of Zenon™ Alexa Fluor™ 488 Human IgG labeling reagent (250 µL), and 1 vial of Zenon™ blocking reagent (rabbit IgG, 250 µL)
Quantity50 Reactions
Storage RequirementsStore in refrigerator (2–8°C) and protect from light
Excitation/Emission495/519
Labeling Scale<1 to 20 μg
Product LineZenon™
Label or DyeAlexa Fluor™ 488
Label TypeAlexa Fluor Dyes
ColorGreen
Labeling TargetHuman
Unit Size1 kit
Z25407
also known as Z-25407
Full specifications
1 kit50 Reactions590/617Alexa Fluor™ 594
449.00
Your Price:
***
Kit ContentsContains 1 vial of Zenon™ Alexa Fluor™ 594 Human IgG labeling reagent (250 µL), and 1 vial of Zenon™ blocking reagent (rabbit IgG, 250 µL)
Quantity50 Reactions
Storage RequirementsStore in refrigerator (2–8°C) and protect from light
Excitation/Emission590/617
Labeling Scale<1 to 20 μg
Product LineZenon™
Label or DyeAlexa Fluor™ 594
Label TypeAlexa Fluor Dyes
ColorRed
Labeling TargetHuman
Unit Size1 kit
Z25408
also known as Z-25408
Full specifications
1 kit50 Reactions650/665Alexa Fluor™ 647
453.00
Your Price:
***
Kit ContentsContains 1 vial of Zenon™ Alexa Fluor™ 647 Human IgG labeling reagent (250 µL), and 1 vial of Zenon™ blocking reagent (rabbit IgG, 250 µL)
Quantity50 Reactions
Storage RequirementsStore in refrigerator (2–8°C) and protect from light
Excitation/Emission650/665
Labeling Scale<1 to 20 μg
Product LineZenon™
Label or DyeAlexa Fluor™ 647
Label TypeAlexa Fluor Dyes
ColorFar-Red
Labeling TargetHuman
Unit Size1 kit
Z25451
also known as Z-25451
Full specifications
1 kit50 Reactions650/660APC (Allophycocyanin)
403.00
Your Price:
***
Kit ContentsContains 1 vial of Zenon™ APC Human IgG labeling reagent (125 µL), and 1 vial of Zenon™ blocking reagent (rabbit IgG, 125 µL)
Quantity50 Reactions
Storage RequirementsStore in refrigerator (2–8°C) and protect from light
Excitation/Emission650/660
Labeling Scale<1 to 20 μg
Product LineZenon™
Label or DyeAPC (Allophycocyanin)
Label TypePE & APC
ColorRed
Labeling TargetHuman
Unit Size1 kit
Z25452
also known as Z-25452
Full specifications
1 kit50 ReactionsBiotin-XX
437.00
Your Price:
***
Kit ContentsContains 1 vial of Zenon™ Biotin-XX Human IgG labeling reagent (250 µL), and 1 vial of Zenon™ blocking reagent (rabbit IgG, 250 µL)
Quantity50 Reactions
Storage RequirementsStore in refrigerator (2–8°C) and protect from light
Labeling Scale<1 to 20 μg
Product LineZenon™
Label or DyeBiotin-XX
Label TypeBiotin & Other Haptens
ColorColorless
Labeling TargetHuman
Unit Size1 kit
Z25455
also known as Z-25455
Full specifications
1 kit25 Reactions496/578R-PE (R-Phycoerythrin)
403.00
Your Price:
***
Kit ContentsContains 1 vial of Zenon™ R-PE Human IgG labeling reagent (125 µL), and 1 vial of Zenon™ blocking reagent (rabbit IgG, 125 µL)
Quantity25 Reactions
Storage RequirementsStore in refrigerator (2–8°C) and protect from light
Excitation/Emission496/578
Labeling Scale<1 to 20 μg
Product LineZenon™
Label or DyeR-PE (R-Phycoerythrin)
Label TypePE & APC
ColorRed-Orange
Labeling TargetAntibodies (IgG)
Unit Size1 kit
Showing 6 of 6
Achieve fast, versatile, and reliable fluorophore-, biotin-, or enzyme-labeled IgG primary antibodies with the Invitrogen Zenon™ Human IgG Labeling Kits. These kits utilize Alexa Fluor fluorophores, biotin, or enzymes such as R-phycoerythrin and allophycocyanin, which are attached to monovalent, affinity purified Fab fragments. The Fab fragments, in turn, are directed against and bind with the Fc portion of IgG primary antibodies. Only a small amount of starting material is required, and the method is optimized for efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions. Because the Zenon labeling method is based on immunoselectivity, it does not require the removal of exogenous proteins (such as serum) or amine-containing buffers from the target antibody, simplifying the process.
. Important features of Zenon labeling technology:

• Labeled antibodies are typically ready to use in 10 minutes
• Requires only 1–20 µg primary antibody
• Simple—no purification required
• Flexible — choose from different fluorophores, biotin, or enzymes.

• Can be used in a variety of applications including ICC, IHC, flow cytometry, and cell imaging.

Advantages of using Zenon antibody labeling kits:

Cost savings
Zenon antibody labeling kits offer a cost-conscious and reproducible method of tagging as little as 0.4 µg in 2 µL of primary antibody, with minimal waste of expensive or difficult-to-obtain antibodies, or excessive washing steps that pose the risk of product loss.
Sensitivity
Label your primary antibodies without compromising their antigen binding affinity: Zenon dye- and enzyme-labeled Fab fragments, which are targeted to the Fc tail, are affinity purified during their preparation to ensure high affinity and selectivity for the Fc portion of the corresponding primary antibody. The procedure for chemical labeling of the Zenon Fab fragments protects their Fc-binding site, resulting in more active labeling reagents.
Speed
No purification procedure is required prior to using Zenon Fab fragments in your laboratory applications. Formation of the Fab-antibody complex occurs in fewer than five minutes, followed by a five-minute blocking step. During this time, almost all the primary antibody in the mixture is labeled with the labeled Fab fragments.
Simplicity
The Fab-antibody complexes display fluorescence or enzymatic activity that is similar in intensity to that of directly labeled primary antibodies. Varying the extent of the antibody labeling is as simple as changing the amount of added Zenon labeling reagent during the reaction. Once the labeling complexes are formed, they can used immediately, without need for antibody purification.
Reliability
The Zenon Fab-antibody complex is stable and allows subsequent or simultaneous labeling of different target cells and tissues with different complexes. After staining, an aldehyde-based fixing step may be used to prevent the transfer of different labels between different primary antibodies, preserving the initial staining pattern.
Customization
We offer custom antibody conjugation services that are efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.
For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Store in refrigerator (2–8°C) and protect from light

Figures

Frequently asked questions (FAQs)

Citations & References