Invitrogen™

Zenon™ pHrodo™ iFL IgG Labeling Reagents

Catalog number: Z25611
Invitrogen™

Zenon™ pHrodo™ iFL IgG Labeling Reagents

Catalog number: Z25611
Achieve rapid, scalable, and efficient labeling of internalized antibody with the Invitrogen™ Zenon™ pHrodo™ iFL IgG Labeling Reagents. These pH-sensitive dye conjugates are non-fluorescent outside the cell but fluoresce brightly red or green when inside acidic environments, including those of phagosomes. As a result, pHrodo iFL IgG Labeling Reagents can offer faster and more accurate results than any other phagocytosis assay.
 
Catalog Number
Z25611
Unit Size
240 µL
Species
Human
Labeling Target
IgG
Label or Dye
pHrodo™ iFL Green
Color
Green
Price (USD)
Price: 1,168.00
Your Price:
Availability
-
Full specifications
Quantity
Quantity240 µL
Product TypeLabeling Reagent
Kit ContentsContains 1 vial of Zenon™ pHrodo™ iFL Green human labeling reagent (250 µL).
SpeciesHuman
Label or DyepHrodo™ iFL Green
Label TypepHrodo™ (pH sensitive)
ColorGreen
Product LineZenon™
Emission530
Excitation505
Labeling TargetIgG
Unit Size240 µL
Catalog Number
Unit Size
240 µL
Species
Mouse
Labeling Target
IgG
Label or Dye
pHrodo™ iFL Green
Color
Green
Price (USD)
Price: 1,162.00
Your Price:
Availability
-
Full specifications
Quantity
Quantity240 µL
Product TypeLabeling Reagent
Kit ContentsContains 1 vial of Zenon™ pHrodo™ iFL Green mouse labeling reagent (250 µL)
SpeciesMouse
Label or DyepHrodo™ iFL Green
Label TypepHrodo™ (pH sensitive)
ColorGreen
Product LineZenon™
Emission530
Excitation505
Labeling TargetIgG
Unit Size240 µL
Catalog Number
Unit Size
240 µL
Species
Mouse
Labeling Target
IgG
Label or Dye
pHrodo™ iFL Red
Color
Red
Price (USD)
Price: 1,162.00
Your Price:
Availability
-
Full specifications
Quantity
Quantity240 µL
Product TypeLabeling Reagent
Kit ContentsContains 1 vial of Zenon™ pHrodo™ iFL Red mouse labeling reagent (250 µL).
SpeciesMouse
Label or DyepHrodo™ iFL Red
Label TypepHrodo™ (pH sensitive)
ColorRed
Product LineZenon™
Emission585
Excitation560
Labeling TargetIgG
Unit Size240 µL
Catalog Number
Unit Size
240 µL
Species
Human
Labeling Target
IgG
Label or Dye
pHrodo™ iFL Red
Color
Red
Price (USD)
Price: 1,168.00
Your Price:
Availability
-
Full specifications
Quantity
Quantity240 µL
Product TypeLabeling Reagent
Kit ContentsContains 1 vial of Zenon™ pHrodo™ iFL Red Human labeling reagent (250 µL).
SpeciesHuman
Label or DyepHrodo™ iFL Red
Label TypepHrodo™ (pH sensitive)
ColorRed
Product LineZenon™
Emission585
Excitation560
Labeling TargetIgG
Unit Size240 µL
Showing 4 of 4
Catalog NumberSpecificationsUnit SizeSpeciesLabeling TargetLabel or DyeColorPrice (USD)AvailabilityQuantity
Z25611Full specifications
240 µLHumanIgG pHrodo™ iFL Green Green
Price: 1,168.00
Your Price:
-
Quantity240 µL
Product TypeLabeling Reagent
Kit ContentsContains 1 vial of Zenon™ pHrodo™ iFL Green human labeling reagent (250 µL).
SpeciesHuman
Label or DyepHrodo™ iFL Green
Label TypepHrodo™ (pH sensitive)
ColorGreen
Product LineZenon™
Emission530
Excitation505
Labeling TargetIgG
Unit Size240 µL
Z25609Full specifications
240 µLMouseIgG pHrodo™ iFL Green Green
Price: 1,162.00
Your Price:
-
Quantity240 µL
Product TypeLabeling Reagent
Kit ContentsContains 1 vial of Zenon™ pHrodo™ iFL Green mouse labeling reagent (250 µL)
SpeciesMouse
Label or DyepHrodo™ iFL Green
Label TypepHrodo™ (pH sensitive)
ColorGreen
Product LineZenon™
Emission530
Excitation505
Labeling TargetIgG
Unit Size240 µL
Z25610Full specifications
240 µLMouseIgG pHrodo™ iFL Red Red
Price: 1,162.00
Your Price:
-
Quantity240 µL
Product TypeLabeling Reagent
Kit ContentsContains 1 vial of Zenon™ pHrodo™ iFL Red mouse labeling reagent (250 µL).
SpeciesMouse
Label or DyepHrodo™ iFL Red
Label TypepHrodo™ (pH sensitive)
ColorRed
Product LineZenon™
Emission585
Excitation560
Labeling TargetIgG
Unit Size240 µL
Z25612Full specifications
240 µLHumanIgG pHrodo™ iFL Red Red
Price: 1,168.00
Your Price:
-
Quantity240 µL
Product TypeLabeling Reagent
Kit ContentsContains 1 vial of Zenon™ pHrodo™ iFL Red Human labeling reagent (250 µL).
SpeciesHuman
Label or DyepHrodo™ iFL Red
Label TypepHrodo™ (pH sensitive)
ColorRed
Product LineZenon™
Emission585
Excitation560
Labeling TargetIgG
Unit Size240 µL
Showing 4 of 4
Zenon™ pHrodo™ iFL Green-labeled or Red-labeled Fab fragments (i.e. labeling reagent) are directed against the Fc portion of an intact IgG primary antibody to form a labeling complex. Formation of the Fab–antibody complex occurs in fewer than 5 minutes. Because the pHrodo™ iFL dyes dramatically increase in fluorescence as the pH of their surroundings becomes more acidic, the antibodies coupled with the Zenon™ pHrodo™ iFL IgG Labeling Reagents provide an excellent measure of endocytic activity based on the acidification of the labeled antibodies as they are ingested by the cell.

The Zenon™ pHrodo™ iFL IgG Labeling Reagents contain sufficient material to label one to four 96-well plates, depending on the Zenon™ labeling reagent concentration used. Only a small amount of starting material is needed, and the method is optimized for efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions.

Other advantages of the pH-sensitive Zenon™ pHrodo™ iFL IgG Labeling Reagents include:

Simplicity — No reagent purification is required
Accuracy — No wash steps or quenchers are needed, minimizing cross-reactivity or background
Speed — Labeled antibodies are typically ready to use in 10 minutes
Specificity — Dyes are designed for the specific detection of phagocytosis and endocytosis


Preserve primary antibody function and affinities
Reactive dye labeling of primary antibodies can have unpredictable and undesirable outcomes. One of these is reduced binding affinity due to label addition in the binding pocket. The Zenon™ antibody labeling approach, which is targeted to the Fc tail, resolves this potential issue.

Try custom antibody conjugates
If the antibody conjugate you need is not available as one of our stocked products, we will prepare a custom antibody conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

Contents & Storage

Store in refrigerator (2°C to 8°C) and protect from light