ZOOM® IPGRunner™ Combo Kit

Catalog number: ZM0002

 Related applications: Protein Gel Electrophoresis | Protein Purification & Isolation

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Description

The ZOOM® IPGRunner™ system is the first system to offer oil-free, 2D electrophoresis in a mini-gel format. First dimension separation isoelectric focusing (IEF) is typically complete in less than three hours. The mini-gel design of the system is easy to handle, eliminates mineral oil overlays, and allows processing of up to 12 samples at once for high-throughput usage.

The ZOOM® IPGRunner™ Combo Kit includes everything needed for first dimension separation:
• ZOOM® IPGRunner™ Mini-Cell
• 10 ZOOM® IPGRunner™ Cassettes
• 12 ZOOM® Strips, pH 3-10NL

For second dimension separation, Novex® ZOOM® gels and the XCell SureLock® Mini-Cell are recommended (both are available separately).

ZOOM® IPGRunner™ Mini-Cell
The ZOOM® IPGRunner™ Mini-Cell consists of a Zoom® IPGRunner Core, a mini-cell chamber, and a lid. The ZOOM® IPGRunner™ Core can be used to perform isoelectric focusing of up to 12 IPG strips (ZOOM® Strips) loaded in two ZOOM® IPGRunner™ Cassettes.

ZOOM® IPGRunner™ Cassette
The ZOOM® IPGRunner™ Cassette is used to perform oil-free rehydration and isoelectric focusing of up to six 7.0 cm IPG strips (ZOOM® Strips) without requiring separate units for rehydration and isoelectric focusing. The ZOOM® IPGRunner™ Cassettes are disposable.

ZOOM® Strips
ZOOM® Strips are 7 cm long and contain a thin layer of polyacrylamide gel with a fixed pH gradient. Each ZOOM® Strip is clearly labeled with a unique identifying number, pH range, and orientation marks (Figure 3). ZOOM® Strips are supplied attached to a tri-fold card (Figure 4) for easy access and removal.

Novex® ZOOM® Gels (available separately)
ZOOM® Gels are pre-cast polyacrylamide gels designed for 2D analysis of proteins following isoelectric focusing with ZOOM® Strips. They are recommended for use with our XCell SureLock® Mini-Cell. ZOOM® Gels contain an IPG well and a molecular weight marker well. The IPG well is designed to accommodate the 7.0 cm ZOOM® Strips. Two types of ZOOM® Gels are available separately:

NuPAGE® Novex® 4–12% Bis-Tris ZOOM® Gel

Novex® 4–20% Tris-Glycine ZOOM® Gel
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): XCell SureLock® Mini, ZOOM® IPGRunner™ Mini
Gel Compatibility: ZOOM® IPG Strips
Gel Size: 7 cm strips
Product Size: 1 kit

Contents & storage

The ZOOM® IPGRunner™ Mini-Cell includes the ZOOM® IPGRunner™ Buffer Core and Lid, Gel Tension Wedge, Buffer Dam, and the Mini- Cell chamber. The ZOOM® IPGRunner™ Combo Kit includes the ZOOM® IPGRunner™ Mini-Cell, electrode wicks, sealing tape, 10 ZOOM® IPGRunner™ Cassettes, and 12 ZOOM® Strips, pH 3-10 NL. The ZOOM® IPGRunner® Retrofit Kit for XCell SureLock™ Mini-Cell includes the ZOOM® IPGRunner™ Buffer Core and Lid. The ZOOM® IPGRunner™ Cassettes include electrode wicks and sealing tape.

Documents

Manuals & protocols

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